Running Protocols; Choosing A Block To Run The Protocol On; Setting Up The Temperature Control Method; Setting Up A Block-Control Protocol - BIO RAD PTC-200 Operation Manual

In either instance, after you press «Proceed», a screen similar to the following
example will be displayed:
The top line of the screen will identify the selected protocol (2-STEP in the example).
The other lines on the screen will request information needed to set up the temperature
control method (explained below).

Choosing a Block to Run the Protocol On

If a Dual Alpha unit is installed (or if networked Alpha units are available; see chapter
9), one of the blocks must be designated to run the protocol. The first available block
is automatically designated when a protocol is chosen. Press «Block» to choose a dif-
ferent block. The selected block's letter is identified in the upper right-hand corner of
the screen:
The status indicator light for the selected block will flash green.

Setting Up the Temperature Control Method

When the protocol is selected (and the correct block is designated for a Dual Alpha unit),
one or more screens will be displayed. These screens will ask for information needed to
set up the block's temperature control method. The DNA Engine cycler can control the
block's temperature in two different ways: block control or calculated control. Chapter
6 explains in detail how these methods work and their implications for protocol design.
The following describes how to respond to the screens that are displayed for each con-
trol method. When these screens have been dealt with, the protocol will begin to run.

Setting Up a Block-Control Protocol

A single screen asking about use of the heated lid will be displayed:
Select Yes or No, then press «Proceed». The protocol will begin running.
Tech Support: 1-800-4BIORAD • 1-800-424-6723 • www.bio-rad.com
Run: 2-STEP
Vessel Type:
_TUBES Plate
Run: 2-STEP o n A
Vessel Type:
_TUBES Plate
Run: ICEBUCKET
Use heated lid?
Yes _NO

Running Protocols

5-3