Setting Up A Calculated-Control Protocol - BIO RAD PTC-200 Operation Manual

DNA Engine Operations Manual

Setting Up a Calculated-Control Protocol

Three screens will be presented:
• A screen asking for sample vessel information:
Select from the options shown (may include tubes, plates, and slides), then press
«Proceed». Choose Plate for polycarbonate microplates and Tube for polypropylene
tubes or polypropylene microplates. For blocks that hold only tubes, choose Thin or
Thick, depending on the type of tube loaded.
Note: See "Using Calculated Control" in the Slide Chambers Alpha Unit Operations
Manual for information on specifying slide format.
• A screen asking for the sample reaction volume (last line in example below):
Use the keypad to enter a sample volume in microliters, then press «Proceed». If
sample vessels are sealed with oil or wax, include the volume of the oil or wax in the
total sample volume entered.
Note: Specify 10 µl for any volume less than 10 µl.
Note: Verify that your vessel sealing system is adequately tight before performing 5 µl
reactions.
• A screen asking about use of the heated lid. Select Yes or No, then press
«Proceed». The protocol will begin running.
5-4 Tech Support: 1-800-4BIORAD • 1-800-424-6723 • www.bio-rad.com
Run: 2-STEP
Vessel Type:
_TUBES Plate
Run: 2-STEP
Vessel Ty p e : TUBES
Volume (m l ) : 10