Entering A Gradient Step - BIO RAD PTC-200 Operation Manual

DNA Engine Operations Manual
Enter the hold time for the step (30 seconds is used in the example below):
Note: If a hold time of zero (0) is entered, the DNA Engine cycler will hold the block
atthe target temperature indefinitely.
Press «Proceed». The hold time will move to the second line of the screen, and a con-
firmation menu will be displayed on the last line:
Select one of the displayed choices, then press «Proceed»:
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•
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4 Tip: Avoid programming many short holds of only a few seconds each. This can overheat
the block, causing the "HS Overheating" or "PS Overheating" error messages to be
displayed and triggering automatic Alpha unit shutdowns if the block exceeds its maximum
allowable temperature.

Entering a Gradient Step

When you reach the step at which a gradient is desired, select Gradient from the
Enter Menu and then press the «Proceed» key. For the purpose of this example the
step in which the gradient will be entered shall be Step 2:
6-8 Tech Support: 1-800-4BIORAD • 1-800-424-6723 • www.bio-rad.com
Enter: CUSTO M 1
1= 92.0
Time:
Enter: CUSTO M 1
1= 92.0 f o r 0 : 3 0
OK?: _YES No Option
Yes accepts the step and displays the Enter Menu again. Use the Enter
Menu to enter the next step in the program.
No allows reentry of the target temperature and hold time for the step.
Option displays the Options Menu (see "Modifying a Program Step with
the Options," p. 6-15).
Enter: CUSTO M 1
Step 2= Temp Goto
_GRADIENT
Ramp
30
End