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BIO RAD PTC-200 Manuals
Manuals and User Guides for BIO RAD PTC-200. We have
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BIO RAD PTC-200 manual available for free PDF download: Operation Manual
BIO RAD PTC-200 Operation Manual (113 pages)
DNA Engine Thermal Cycler
Brand:
BIO RAD
| Category:
Laboratory Equipment
| Size: 0 MB
Table of Contents
Table of Contents
4
Explanation of Symbols
5
Safety Warnings
5
Safe Use Guidelines, Electromagnetic Interference, and FCC Warning
7
Documentation Conventions
7
The DNA Engine Peltier Thermal Cycler
8
Introduction
8
2 Layout and Specifications
12
Control Panel
13
Front View
13
Back View
14
Bottom View
14
Alpha Units
15
Single-Block Models
15
Dual-Block Models
15
Slide Block
15
Moto Alpha Unit
15
Specifications
16
Gradient Specifications
17
3 Installation
18
Environmental Requirements
19
Packing Checklist
19
Setting up the DNA Engine Cycler
19
Air Supply Requirements
20
Ensuring an Adequate Air Supply
20
Ensuring that Air Is Cool Enough
21
Power Supply Requirements
20
Requirements for Robotics Installations
21
4 Operation
22
Turning the DNA Engine Cycler on
23
Understanding the Main Menu
23
Operation
24
Using the Data Ports
24
Using the Control Panel
24
Operation Keys
24
Status Indicator Lights
24
Operating Alpha Units
25
Installing an Alpha Unit
25
Removing an Alpha Unit
26
Opening an Alpha Unit
26
Closing an Alpha Unit
26
Selecting the Correct Sample Vessel
27
0.5 Ml Tubes
27
Microplates
27
Thin-Walled Vs. Thick-Walled Tubes
27
Sealing Sample Vessels
28
Sealing with Oil or Wax
28
Sealing with the Hot Bonnet Lid
28
Adjusting the Hot Bonnet Lid's Pressure
29
Loading Sample Vessels into the Block
30
Using Oil to Thermally Couple Sample Vessels to the Block
31
Appendix 4-A: Tube, Microplate, and Sealing System Selection Chart
32
Appendix 4-B: Safety Warning Regarding Use of
33
5 Running Protocols
36
Running a Protocol
37
Choosing a Stored Protocol to Run
37
Running Protocols
38
Choosing a Block to Run the Protocol on
38
Setting up the Temperature Control Method
38
Setting up a Block-Control Protocol
38
Setting up a Calculated-Control Protocol
39
Reading the Runtime Screen
40
Switching between the Runtime Screen and the Main Menu
41
Reading the Protocol Completion Screen
41
Printing a Log for a Running Program
42
Manually Stepping through a Protocol
42
Pausing a Running Protocol
42
Stopping a Running Protocol
43
Resuming a Protocol after a Power Outage
43
Using the Instant Incubation Feature
44
6 Creating Programs
46
The Elements of a Program
47
Creating Programs
48
Designing a New Program
48
Translating a Protocol into a Program
48
Using the Goto Step to Write Short Programs
48
Choosing a Temperature Control Method
49
Calculated Control
49
Block Control
49
Modifying Block-Control Programs for Calculated Control
50
Modifying a Program Designed for a Different Machine
50
Entering a New Program
50
Initiating the Program
50
Naming the Program
51
Choosing a Temperature Control Method
51
Entering the Program's Steps
51
Entering a Temperature Step
52
Entering a Gradient Step
53
Editing a Gradient Step
55
Reviewing a Gradient Program
55
Using the Gradient Calculator
56
Entering a Ramp Step
57
Entering a Goto Step
58
Entering the End Step
59
Modifying a Program Step with the Options
60
Entering an Increment Option
60
Entering an Extend Option
61
Revising During Programming
63
To Change the Last Value Entered or Menu Option Chosen
63
To Change All the Values in the Step Being Entered
63
To Change Values for Earlier Steps in the Program
63
Deleting a Program
64
Keeping a Permanent Record of Programs
64
7 Editing Programs
66
Editing a Stored Program
67
Initiating Editing
67
Editing the Program
68
Inserting a New Step
69
Deleting a Step
69
Adding an Option
69
Saving an Edited Program
69
Cancelling Editing Changes
70
8 Using the Utilities
72
Locating a Stored Program
73
File Utilities
73
Using the Utilities
74
Creating a Folder
74
Assigning a Password to a Folder
74
Deleting a Folder
75
Copying a Program
75
Renaming a Program
76
Moving a Program
76
Deleting a Program
76
List Utilities
76
Viewing a Program in the LCD Window
77
Printing a Program
77
Setup Utilities
78
Choosing a Remote Port
78
Choosing a Printer Port
79
Choosing a Minimum Block Temperature for the Hot Bonnet Lid
79
Choosing a Temperature Control Method for the Hot Bonnet Lid
79
Determining the Software Version Number
80
Networking
82
10 Maintenance
88
Cleaning the DNA Engine Cycler
89
Cleaning the Chassis and Block
89
Cleaning the Air Vents
89
Cleaning Radioactive or Biohazardous Materials out of the Block
90
Changing the Fuses
90
Troubleshooting
92
Appendix A: Warranties
101
Appendix A: Warranties
101
Appendix B: Factory-Installed Protocols
101
Index
103
Declaration of Conformity
111
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