Ensuring That Air Is Cool Enough; Requirements For Robotics Installations - BIO RAD PTC-200 Operation Manual

DNA Engine Operations Manual

Ensuring That Air Is Cool Enough

• Do not position two or more DNA Engine cyclers (or other thermal cyclers) so that
the hot exhaust air of one blows directly into the air intake vents of another.
• Make sure the DNA Engine cycler receives air that is 31°C or cooler by measuring
the temperature of air entering the machine through its air intake vents.
Place the DNA Engine cycler where you plan to use it, and turn it on. Try to reproduce
what will be typical operating conditions for the machine in that location, particularly
any heat-producing factors (e.g., nearby equipment running, window blinds open,
lights on). Run a typical protocol (e.g., 2-Step) for 30 minutes to warm up the DNA
Engine cycler, then measure the air temperature at the back air intake vents. If more
than one machine is involved, measure the air temperature for each.
If the air intake temperature of any machine is warmer than 31°C, use table 3-1 to
troubleshoot the problem. Some experimentation may be required to determine the
best solution when more than one cause is involved. After taking steps to solve the
problem, verify that the temperature of the air entering the air intake vents has been
lowered, using the procedure outlined above.

Requirements for Robotics Installations

Robotics installations require special attention to airflow and air temperature. Typically in
these installations, DNA Engine cyclers and other thermal cyclers are crowded into a
small area, along with other heat-generating equipment. Overheating can quickly occur
when many of these machines are operating at once, unless preventive measures are
taken.
Follow the procedures described above to ensure adequate airflow and an air intake
temperature of 31°C or cooler. Air intake temperature must be verified by measurement.
Do not use oil to thermally couple sample vessels to the blocks of machines in a robotics
installation. Oil makes plates difficult to remove.
Table 3-1 Troubleshooting Air Supply Problems
Cause
Air circulation is poor.
Ambient air temperature is
high.
Machine is in warm part of
room.
Machines are crowded.
3-4 Tech Support: 1-800-4BIORAD • 1-800-424-6723 • www.bio-rad.com
Possible Remedies
Provide more space around machine or adjacent room ventilation.
Adjust air conditioning to lower ambient air temperature.
Move machine away from, or protect machine from, such heat
sources as radiators, heaters, other equipment, or bright sunlight.
Arrange machines so that warm exhaust air does not enter intake
vents.