Calculation Of Sample Concentration - Hitachi 902 Service Manual

Calculation of Sample Concentration

Given below is an example where the measured absorbance of a sample is 0.1637.
Cx = {K • (Ax - B) + C
Cx : Concentration of sample
K : Calibration factor
Ax : Absorbance of sample
C : Concentration of standard solution 1
1
B : Absorbance of standard solution 1 (S1ABS)
IF : Instrument constant A (assumed to be 1.0)
A
IF : Instrument constant B (assumed to be 0.0)
B
Cx = [ {202 • (0.1637 - 0.0447) + 0.0 }
In the above formula, the result of calculation is multiplied by the exponential part (10
was ignored in the calculation of K value and the product is rounded off to match scaling. Thus,
the final Cx value is 2.4.
• 1F + IF
}
1 A B
1.0 + 0 ] × 10
•
= 2.39 → 2.4
-1
9 - 10
-1
), which