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2.6
2.6
Measurement Function
Measurement
2.6.1 Measurement
2.6.1 Measurement
Mode
Mode
2.6.2 Quantitative
2.6.2 Quantitative
Calculation
Calculation
Function
With the UV
With the UV Solutions program, the following measureme
Solutions program, the following measurement nt
modes are available.
modes are available.
Quantitative
Quantitative calculation
• •
Wavelength
Wavelength scan
• •
Time scan
Time
scan
• •
The spectrophotometer has a function to prepare a calibration
The spectrophotometer has a function to
curve and calculate the
curve and calculate the concentration.
(1)
(1)
Preparation of of Calibration
Preparation
(a) Up
(a)
Up to to 20
curve is prepared by connecting the
curve is prepared by connecting the obtained values
with polygonal lines, or a regression line is prepared
with polygonal lines, or a
using
using least-squares
measure each standard up to 20 times and prepare a
measure each standard up to 20 times and prepare a
calibration curve based on the obtained average
calibration curve based on the obtained average
values.
values.
(b) A calibratio
(b)
A calibration curve
particular standard.
particular standard.
(c) It is
(c)
It is possible
possible to displ
screen and read out a value of each point using the
screen and read out a value of each point using the
cursor.
cursor.
(d) A calibr
(d)
A calibration cur
(e) A calibratio
(e)
A calibration curve
value.
value.
(f) (f)
The result
The
result of me
displayed on
displayed
to carry out measuremen
to carry out
calibration curve.
calibration curve.
(g) According
(g)
According to the
automatically, judgment can be
automatically, judgmen
matching between standard values and a calibration
matching between standard values and a
curve.
curve.
(h) It is possi
(h)
It is possible to p
two/three-wavelength
two/three-wavel
calculation
scan
concentration.
Calibration Curve
Curve
20 standards
standards can
can be
be measured.
regression line is prepared
least-squares calculation.
calculation. It i
n curve can be
can be redrawn
to display a
ay a calibration
calibration curve o
ation curve can
ve can be outp
be output to
n curve can be
can be drawn by
of measurement
asurement and
on the
the same
same screen.
screen. It is
measurement while observing the
t while observing the
to the coefficient
coefficient of de
t can be made on the degree of
ble to prepare a
repare a calibration
calibration curve by
ength calculation.
calculation.
2 - 9
2 - 9
prepare a calibration
measured. A A calibration
calibration
obtained values
It is s also
also possible
possible to to
redrawn by remeasu
by remeasuring a
ring a
curve on the
n the
ut to the prin
the printer.
ter.
drawn by entering
entering its factor
its factor
and a ca
a calibration
libration curve
curve are
It is therefore
therefore possible
possible
of determination
termination calculated
calculated
made on the degree of
calibration
curve by using
using
2.6.1
2.6.1
are
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