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• UV/Vis
The default settings for the two cursors used to monitor specific
wavelengths are 300 nm for λ1 and 700 nm for λ2. The user may
elect to have the HiAbs on (automatic utilization of the 0.2 mm
path). An additional option is to elect to normalize the data and
spectra using the absorbance value of the wavelength between
400 nm and 700 with the lowest andabsorbance value.
• Microarray
The default setting is ssDNA-33 for the nucleic acid. The default
settings remain Dye 1 set to Cy3, Dye 2 set to Cy5, with
absorbance normalized to the absorbance value at 750nm.
Other options include RNA-40, ssDNA-33 and Other. There are
several hard-coded dye choices including common Alexa fluor
dyes. See the explanation of the Dye/Chromophore Editor later in
this section for information about adding custom dyes and
chromophores.
• A280
There are six sample type options available for purified protein
analysis and concentration measurement. The default setting is
Other protein (E1%). See Section 8 for additional information about
each sample type option. Note: Software versions 3.5.1 and higher
include the option to select whether or not to have the data and
spectrum normalized to the absorbance value at 340 nm.
• Proteins and Labels
There are six sample type options available for purified protein
analysis and concentration measurement. The default setting is
Other protein (E1%).
The user may also elect whether or not to use a bichromatic
normalization of the 280 nm absorbance value to the absorbance
value at 340 nm.
The default dye setting remains Dye 1 set to Cy3 with absorbance
normalized at 750nm.
Utilities and Diagnostics
This module is used to both confirm that the instrument is performing
within the pathlength calibration specifications and help troubleshoot
operational problems with the instrument. Note: The calibration check
utility is available in software versions 3.5.1 and higher.
Section 3-General Operation
3-7
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