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18. Maintenance and Warranty
Cleaning
The primary maintenance requirement of the NanoDrop 1000
Spectrophotometer is keeping the measurement pedestal surfaces
clean. Upon completion of each sample measurement, wipe the
sample from the upper and lower pedestals to prevent sample
carryover and avoid residue buildup. It is also recommended that
both measurement pedestals be cleaned with de-ionized water upon
completion of a testing series.
Apply 5 ul of dH
1.
Lower the upper pedestal arm to form a liquid column; let it sit
2.
for approximately 2-3 minutes.
Wipe away the water from both the upper and lower pedestal
3.
with a clean lab wipe.
Note: Typically dH
dried on the optical pedestals. There are a few cases (i.e. dried
proteins) that may require a more rigorous cleaning protocol. For
these cases, we recommend that 0.5M HCl be substituted for the 5 ul
of dH
0. Please follow an HCl application with 5 ul of dH20 to ensure
2
any residual HCl is removed. Note: Do not use a squirt bottle to apply
de-ionized water or HCl.
Calibration
Pathlength (Accuracy) Calibration Check)
It is good practice to verify the calibration every six months using CF-
1 calibration fluid.. This will verify the pathlength accuracy of the
instrument. The calibration check procedure is found within the
Utilities and Diagnostics module.
Wavelength
Each time the software is started, the wavelength is auto-calibrated
based on known peaks in the xenon lamp spectra. No calibration is
required by the user.
Parts That Require Replacement
In general, the xenon flash lamp is the only part that will need to be
replaced. The lamp has a lifespan of at least 30,000 measurements.
When the flash lamp fails, the light output will become very erratic or
0 solution onto each bottom pedestal.
2
0 is sufficient for removal of samples that have
2
18-1
Section 18- Maintenance and Warranty
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