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Section 7- UV/Vis
pathlength. The wavelengths can be set by dragging the cursor,
using the up/down arrows or typing in the desired wavelength.
Baseline: the absorbance of the user selectable-baseline (horizontal)
cursor. The user may drag this cursor to a new vertical position to
create a new baseline. The absorbance value of the baseline is
subtracted from the absorbance of the spectrum.
Max Absorbance: used to rescale the upper limit of the vertical axis.
Hi Abs: samples with high absorbance (up to 75 A equivalent @ 10
mm path) can be measured directly (applicable for instruments with
serial numbers >500 only or that have been field-retrofitted). This
capability is selected by choosing the 'Hi Abs' button on the header
bar. When this is selected, the absorbance is measured using the
short path (0.2mm) and plotted as a red line normalized to a 0.1mm
path for easier visual comparison. Sample ID label will be stored with
sample data in the file folder "C:\NanoDrop Data\User name\ HiAbs".
This data cannot be imported into the Data Viewer but can be opened
with Excel type spreadsheets. This feature may be selected as the
default option using the User Preferences module.
Normalize: This is a user-selectable feature in this module. If
selected, the software will automatically normalize the spectrum
based on the lowest absorbance value in the range 400-750 nm. This
feature may be selected as the default option using the User
Preferences module.
7-2
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