Thermo Scientific NanoDrop 1000 V3.7 User Manual page 4

Spectrum Overlay Control ............................................................. 8-4
9. Proteins & Labels ........................................................................ 9-1
Fluorescent Dye Selection ............................................................ 9-1
Sample Volume Requirements...................................................... 9-1
Pedestal Reconditioning................................................................ 9-2
Measurement Concentration Range ............................................. 9-2
Unique Screen Features ............................................................... 9-2
Baseline Type................................................................................ 9-4
10. Protein BCA ............................................................................... 10-1
Sample Volume Requirements.................................................... 10-1
Pedestal Reconditioning.............................................................. 10-1
Measurement Concentration Range ........................................... 10-1
BCA Kits, Protocols, and Sample Preparation ............................ 10-2
Unique Screen Features ............................................................. 10-2
Making BCA Measurements........................................................ 10-3
Standard Curve Features ............................................................ 10-5
Delete Standard Points ............................................................... 10-5
Exiting the BCA Module .............................................................. 10-6
11. Protein Lowry............................................................................. 11-1
Sample Volume Requirements.................................................... 11-1
Pedestal Reconditioning.............................................................. 11-1
Measurement Concentration Range ........................................... 11-1
Modified Lowry Kits, Protocols, and Sample Preparation ........... 11-2
Unique Screen Features ............................................................. 11-2
Making Lowry Measurements ..................................................... 11-3
Standard Curve Features ............................................................ 11-5
Delete Standard Points ............................................................... 11-5
Exiting the Lowry Module ............................................................ 11-6
12. Protein Bradford ........................................................................ 12-1
Sample Volume Requirement ..................................................... 12-1
Pedestal Reconditioning.............................................................. 12-1
Measurement Concentration Range ........................................... 12-2
Bradford Kits, Protocols, and Sample Preparation...................... 12-2
Unique Screen Features ............................................................. 12-3
Making Bradford Protein Measurements..................................... 12-3
Standard Curve Features ............................................................ 12-5
Delete Standard Points ............................................................... 12-6
Exiting the Bradford Module ........................................................ 12-7
13. Protein Pierce 660 nm............................................................... 13-1
Unique Screen Features ............................................................. 13-1
Making Pierce 660 nm Protein Measurements ........................... 13-2
Standard Curve Features ............................................................ 13-4
Delete Standard Points ............................................................... 13-5
14. Cell Cultures .............................................................................. 14-1
Sample Size Requirements......................................................... 14-2
Cell Suspension Concentrations ................................................. 14-2
Sample Homogeneity .................................................................. 14-2
Decontamination of Measurement Pedestals ............................. 14-2
15. Archived Data and Data Viewer ............................................... 15-1
Archive File Creation ................................................................... 15-1
Data Storage Hierarchy............................................................... 15-2
Data Viewer ................................................................................. 15-2
Archive File Converter................................................................. 15-9
Opening Archived Data with Spreadsheet Programs................ 15-10
16. Calibration Check ...................................................................... 16-1
Procedure .................................................................................... 16-1
17. Troubleshooting ........................................................................ 17-1