Making Pierce 660 Nm Protein Measurements - Thermo Scientific NanoDrop 1000 V3.7 User Manual

Section 13- Protein Pierce 660 nm
View Standard Curve (F8): selecting this button allows the user to
view the standard curve at any time.
Sample Type: choices are Reference, Standards 1-7, and Sample.
The software will guide you to measure your reference, then at least
one standard before allowing measurement of samples. Reference
samples refer to the dye reagent without any protein added (ie the "0"
sample).
Replicate #: counter for tracking replicate number during reference
and standard measurement.
Reset all Standards (F11): clears all replicates of all standards.
Reset 1 Standard (F12): clears all replicates of the selected
standard.
Absorbance at 660 nm: the protein-reagent complex's absorbance
at 660 nm for the 1mm pathlength.
Cursor λ and Absorbance: this feature allows the user to adjust the
cursor wavelength and view the corresponding absorbance. The
cursor wavelength can be set by the user. Note: The user-selected
wavelength and absorbance are not utilized in any calculations.
ug/ml: concentration of the sample (unknown).

Making Pierce 660 nm Protein Measurements

A standard curve is required by the software every time the Pirece
Protein 660 nm assay is run. Although curves can be saved and
reloaded in the NanoDrop 1000 Spectrophotometer software, it is
recommended that the user follow manufacturers' guidelines
regarding the use of saved curves when running this assay.
Additionally, a standard curve 'set-up' may be reloaded. This feature
will recall the respective standard series used in a previously saved
standard curve. Both single and multi-point standard curve
generation is incorporated into the software.
A standard curve can be developed using a reference (reagent only –
no protein) and a single replicate of one standard. The multi-point
standard curve generator allows a maximum of five replicates for up
to seven different standards. There is no set order in which standards
must be run.
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