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• RNA: 40 ng-cm/ul
For the NanoDrop 1000 Spectrophotometer, path lengths of 1.0 mm
and 0.2 mm are used compared to a standard spectrophotometer
using a 10.0 mm path. Thus, the NanoDrop 1000 Spectrophotometer
is capable of measuring samples that are 50 times more concentrated
than can be measured in a standard spectrophotometer.
Note: Absorbance data shown in archive files are represented as
displayed on the software screen. For Nucleic Acid, Protein A280 and
Proteins and Labels modules, data are normalized to a 1.0 cm (10.0
mm) path. For MicroArray, UV-Vis, Protein BCA, Protein Bradford,
Protein Lowry and Cell Culture modules the data are normalized to a
0.1 cm (1.0 mm) path. For high absorbance UV-Vis samples, data
are normalized to a 0.1mm path.
Solvent Compatibility
The NanoDrop 1000 Spectrophotometer is compatible with most
solvents typically used in life science laboratories. These include:
methanol, ethanol, n-propanol, isopropanol, butanol, acetone, ether,
chloroform, carbon tetrachloride, DMSO, DMF, Acetonitrile, THF,
toluene, hexane, benzene, sodium hydroxide, sodium hypochlorite
(bleach), dilute HCl, dilute HNO3, dilute acetic acid.
All forms of Hydrofluoric Acid (HF) are incompatible, as the fluoride
ion will dissolve the quartz fiber optic cable.
Decontamination of Measurement & Optical Surfaces
If decontamination is necessary, a sanitizing solution, such as a 0.5%
solution of sodium hypochlorite (1:10 dilution of common commercial
bleach solutions – freshly prepared), can be used to ensure that no
biologically active material is present on the measurement pedestals.
The metal fiber optic fittings are made from 303 stainless steel and
are resistant to most common laboratory solvents (see "Solvent
Compatibility" appendix).Note: Do not use a squirt bottle to apply the
diluted bleach.
Setting Up a Dymo 400 Label Writer Printer
To set DYMO default label to print with the NanoDrop 1000
Spectrophotometer:
1. Open Control Panel, then Printers and Faxes
2. Right click on Dymo printer then select Properties
3. Under General tab, choose 'Printing Preferences' then
'Advanced'. Then set the following:
•
Paper Size: 30256
19-3
Section 19- Appendices
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