Microarray; Fluorescent Dye Selection; Sample Volume Requirements - Thermo Scientific NanoDrop 1000 V3.7 User Manual

Section 6- MicroArray

6. MicroArray

The capability to pre-select viable fluorescent-tagged hybridization
probes for gene expression in micro arrays can eliminate potentially
flawed samples and improve research effectiveness. The NanoDrop
1000 Spectrophotometer measures the absorbance of the fluorescent
dye, allowing detection at dye concentrations as low as 0.2 picomole
per microliter.

Fluorescent Dye Selection

There are currently nine fluorescent dyes that are hard-coded for use
with the MicroArray module (see table below). Users can also enter &
save fluorescent dyes not coded within the NanoDrop 1000
Spectrophotometer software using the 'Dye/Chromophore Editor'
button found in the main menu. Dyes can be selected using the scroll
arrows or by highlighting the Dye 1 or Dye 2 box. The respective
absorbance wavelength, extinction coefficient, and 260nm and 280nm
% corrections will be automatically utilized for measurement and
concentration calculation. The default settings remain Dye 1 set to
Cy3 and Dye 2 set to Cy5. In addition to the fluorescent dyes
available from the drop-down menu, an option entitled 'None' is also
available. Selecting 'None' disables the respective calculations &
numeric displays corresponding to that dye.
Note: Please refer to the dye manufacturer for the appropriate
correction factors for user entered dyes.

Sample Volume Requirements

Field experience has indicated that 1 ul samples are sufficient to
ensure accurate and reproducible results when measuring aqueous
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