Thermo Scientific NanoDrop 1000 V3.7 User Manual page 55

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Section 11-Protein Lowry
Step 1: Measure the
'Reference' (Lowry
reagent – a 'zero'
Standard)
Note: The software
will guide the user
with instructions in the
large text box on the
right side of the
screen.
Step 2: Measure
Standards
Up to 5 replicates of
each of up to 7
standards can be
measured.
The software will not
allow measurement of
samples until a
minimum of 1
standard and
references – or 2
standards – are
measured.
Polynomial curve
fitting requires more
standard points.
11-4

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