Table of Contents
Advertisement
Solvent Information
Observe the following recommendations on the use of solvents.
• Follow recommendations for avoiding the growth of algae, see
• Small particles can permanently block capillaries and valves. Therefore,
• Avoid or minimize the use of solvents that may corrode parts in the flow
Solvent compatibility for stainless steel in standard LC systems
Stainless steel is inert against many common solvents. It is stable in the
presence of acids and bases in the pH range specified for standard HPLC (pH 1
– 12.5 ). It can be corroded by acids below pH 2.3 . In general following
solvents may cause corrosion and should be avoided with stainless steel:
• Solutions of alkali halides, their respective acids (for example, lithium
• High concentrations of inorganic acids like nitric acid, sulfuric acid and
• Halogenated solvents or mixtures which form radicals and/or acids, for
• Chromatographic grade ethers, which can contain peroxides (for example,
1260 Infinity QuatPump VL User Manual
Growth in HPLC Systems"
always filter solvents through 0.4 µm filters.
path. Consider specifications for the pH range given for different materials
like flow cells, valve materials etc. and recommendations in subsequent
sections.
iodide, potassium chloride, and so on) and aequous solutions of halogenes
organic solvents especially at higher temperatures (replace, if your
chromatography method allows, by phosphoric acid or phosphate buffer
which are less corrosive against stainless steel).
example:
→ 2 COCl
2 CHCl
+ O
3
2
This reaction, in which stainless steel probably acts as a catalyst, occurs
quickly with dried chloroform if the drying process removes the stabilizing
alcohol.
THF, dioxane, di-isopropylether) such ethers should be filtered through dry
aluminium oxide which adsorbs the peroxides.
on page 50
+ 2 HCl
2
Using the Pump
Solvent Information
"Algae
4
51
Advertisement
Chapters
Table of Contents
