Algae Growth In Hplc Systems; How To Prevent And-Or Reduce The Algae Problem - Agilent Technologies 1260 Infinity VL User Manual

4
Using the Pump

Algae Growth in HPLC Systems

Algae Growth in HPLC Systems

The presence of algae in HPLC systems can cause a variety of problems that
may be incorrectly diagnosed as instrument or application problems. Algae
grow in aqueous media, preferably in a pH range of 4-8. Their growth is
accelerated by buffers, for example phosphate or acetate. Since algae grow
through photosynthesis, light will also stimulate their growth. Even in distilled
water small-sized algae grow after some time.
Instrumental Problems Associated With Algae
Algae deposit and grow everywhere within the HPLC system causing:
• Blocked solvent filters or deposits on inlet or outlet valves resulting in
• Small pore high pressure solvent filters, usually placed before the injector
• PTFE frits blockage leading to increased system pressure.
• Column filters to plug giving high system pressure.
• Flow cell windows of detectors to become dirty resulting in higher noise

How to Prevent and-or Reduce the Algae Problem

• Always use freshly prepared solvents, especially use demineralized water
• Never leave mobile phase in the instrument for several days without flow.
• Always discard old mobile phase.
• Use the amber solvent bottle (Solvent bottle, amber (p/n 9301-1450))
• If possible add a few mg/l sodium azide or a few percent organic solvent to
50
unstable flow, composition or gradient problems or a complete failure of
the pump.
to plug resulting in high system pressure.
levels (since the detector is the last module in the flow path, this problem is
less common).
which was filtered through about 0.2 µm filters.
supplied with the instrument for your aqueous mobile phase.
the aqueous mobile phase.
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