Using The System; How To Clean Optical Surfaces Of Fibrette Optical Component - REPLIGEN CTech SoloVPE PLUS User Manual

CTech™ SoloVPE® PLUS System

7. Using the System

This section provides detailed step-by-step instructions for the most commonly used features and functions of the SoloVPE PLUS
System. The procedures include guidance on the proper use of the hardware and the software, as well as expectations and
limitations of the system.
For more instruction on proper care, refer to CTech SoloVPE and SoloVPE PLUS Best Practices DOC0153 and VPT Support: System
Service Options DOC0333 provided with the system.
7.1 Fibrette® Optical Components
The variable pathlength capability relies on the use of a light-transmitting component that moves in the sample solution, thus
adjusting the pathlength. To reduce the risk of carryover and the time between measurements, the SoloVPE PLUS System has been
designed to use disposable fiber optics called Fibrette Optical Components.

7.1.1 How to Clean Optical Surfaces of Fibrette Optical Component

*Warning: Repligen strongly recommends that Fibrette Optical Components only be used once to avoid the risk of measurement
errors associated with improper cleaning and storage, damage, or carryover. This procedure is provided strictly for support of
noncritical, academic, and non-GxP implementations for which measurement accuracy tolerances have been deemed less
critical.
1. After each use of a Fibrette Optical Component, store them in distilled water in a neoprene tube or a soft container to keep
them wet.
•
Fibrette Optical Component must soak for a minimum of 30 minutes, but no longer than 24 hours.
•
Soaking longer than 24 hours can damage the polyamide coating.
•
Do not soak too many Fibrette Optical Components in a container; otherwise, they will clump together.
2. At the end of the day, properly dispose of the water and fill the tube with cleaning reagent (e.g., IPA, methanol, or ethanol)
and let it soak for 2 to 5 minutes.
3. Pour out the solution and lay Fibrette Optical Component out on a paper towel to dry.
4. Wipe entire length of Fibrette Optical Component with a lint-free wipe. Then spin both ends on a folded lint-free wipe.
5. Place the Fibrette Optical Component back in the clean tube for future use.
7.2 Proper Cover Use
When taking measurements, the Tower Cover must always be in the closed position to avoid issues with ambient light. While the
instrument is largely immune to the effects of room light, the presence of the Tower Cover improves stability, while at the same
time protects users from the motion of the SoloVPE PLUS hardware as it changes pathlengths. When the cover is raised, the Fibrette
Optical Component can be loaded and unloaded into the Fibrette Coupler.
DOC0362 eRev. 1.0 [Publication Date]
User Guide
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