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Related Manuals for REPLIGEN KrosFlo KR2i
Summary of Contents for REPLIGEN KrosFlo KR2i
- Page 1 KrosFlo® KR2i RPM™ System User Guide DOC0304 eRev. [Major Version]1.01.0 [Publication Date]8/9/20238/9/2023 repligen.com © 2023 Repligen Corporation. All rights reserved. The trademarks mentioned herein are the property of Repligen Corporation and/or its affiliate(s) or their respective owners.
- Page 2 Product are expressly disclaimed. Customer shall refer to the terms and conditions of sale governing the transaction for any and all warranties for the Product. Repligen Corporation shall not be liable for errors contained herein or for incidental or consequential damages in connection with the furnishing, performance, or use of this material.
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Page 3: Table Of Contents
User Guide KrosFlo® KR2i Real-Time Process Management (RPM™) System Contents 9.1.1 Connecting the FlowVPX Head to the FlowVPX Standard Mount .................. 26 9.1.2 Connecting the FlowVPX Instrument to the Cary 60 Spectrophotometer and Computer ..........29 9.2.1 Loading the Flow Cell ..............................33 9.2.2 Unloading the Flow Cell .............................. - Page 4 KrosFlo® KR2i Real-Time Process Management (RPM™) System List of tables Table 1. Explanation of user attention phrases ............................7 Table 2. Safety precautions for KrosFlo KR2i RPM System ........................8 Table 3. Performance Specifications ................................9 Table 4. Electrical Input Specifications ..............................9 Table 5.
- Page 5 User Guide KrosFlo® KR2i Real-Time Process Management (RPM™) System Figure 22. Rotate Clamp Handle ................................27 Figure 23. Tighten Mounting Post Clamp Handle ............................ 28 Figure 24. Install FlowVPX Head on mounting post ..........................28 Figure 25. Lower FlowVPX Head on mounting post ..........................28 Figure 26.
- Page 6 Both module types are disposable to eliminate the potential for cross-contamination. This user guide provides guidance for general use of the KrosFlo KR2i RPM System. For further optimization or troubleshooting support, please contact our Customer Service team: customerserviceUS@repligen.com...
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Page 7: Table 1. Explanation Of User Attention Phrases
User Guide KrosFlo® KR2i Real-Time Process Management (RPM™) System 2. About this document This manual uses several different phrases. Each phrase should draw the following level of attention: Table 1. Explanation of user attention phrases Phrase Description Note: Points out useful information. IMPORTANT Indicates information necessary for proper instrument operation. -
Page 8: Table 2. Safety Precautions For Krosflo Kr2I Rpm System
User Guide KrosFlo® KR2i Real-Time Process Management (RPM™) System 3. Safety precautions Table 2. Safety precautions for KrosFlo KR2i RPM System Symbol Description High voltages exist and are accessible. Use extreme caution when servicing internal components. DANGER Remove power from the pump before any cleaning operation is started. -
Page 9: Table 3. Performance Specifications
User Guide KrosFlo® KR2i Real-Time Process Management (RPM™) System 4. System Specifications The tables below outline the performance specifications, electrical requirements, physical attributes, environmental considerations, and compliance information of the major system components. Tubing specifications can be found in section 8.2. Table 3. -
Page 10: Table 5. Physical Specifications
User Guide KrosFlo® KR2i Real-Time Process Management (RPM™) System KR2i TFF System Supply voltage limits 90–260 V @ 50–60 Hz (Universal Input) Current, Maximum 2.2 A @ 115 V , or 1.1 A @ 230 V FlowVPX System Supply voltage limits 100–230 VAC @ 50–60 Hz Current, Maximum 0.6 A... -
Page 11: Table 6. Environmental Specifications
User Guide KrosFlo® KR2i Real-Time Process Management (RPM™) System Weight 1.6 kg Housing Materials Powder-coated Aluminum, Urethane Table 6. Environmental Specifications Specification Value KR2i Main Pump Enclosure Rating IP33 Operating Temperature 0° to 40°C (32° to 104°F) Storage Temperature –25° to 65°C (–13° to 149°F) Humidity (non-condensing) 10% to 90% Altitude... -
Page 12: Table 8. Computer Specifications: Included Tablet
User Guide KrosFlo® KR2i Real-Time Process Management (RPM™) System EN61010-1 (EU Low Voltage Directive) EN61326 (EU EMC Directive) RoHS Directive 2011/65/EU FlowVPX System (including Agilent Cary 60) UL 61010-1, CAN/CSA C22.2 No. 61010-1 EN61010-1 (EU Low Voltage Directive) EN61326 (EU EMC Directive) RoHS Directive 2011/65/EU KONDUiT Conductivity, Temperature, and UV Monitor... -
Page 13: Table 10. System Parts List
User Guide KrosFlo® KR2i Real-Time Process Management (RPM™) System 5. System Configuration and Major Components 5.1 System Parts List Table 10. System Parts List Part Description Quantity KR2i TFF System KrosFlo® Research 2i Pump Drive w/ Integrated Pressure Monitor Microsoft® Surface Pro® with Installed KrosFlo RPM™ Software Suite Auxiliary Component Octopus Cable Pressure Transducer Cable Power Supply Cable... -
Page 14: Table 11. Materials Of Construction
User Guide KrosFlo® KR2i Real-Time Process Management (RPM™) System 6. Materials of Construction Table 11. Materials of Construction Part Number Material KR2i TFF System Process Contact Surfaces Tubing / Reservoir Closures C-Flex® / Pharmapure® Reservoirs Polypropylene Disposable Pressure Transducers Polysulfone Plastic Fittings Polypropylene / Polysulfone FlowVPX Flow Cell Process Contact Surfaces... -
Page 15: Figure 1. Manual Mode Setup Configuration
User Guide KrosFlo® KR2i Real-Time Process Management (RPM™) System 7. Setup and Operation 7.1 Basic Setup Note: See Sections 7.2 through 7.7 for Sample Applications. 1. Mount the TFF system on a flat, horizontal surface with no more than two Pump Heads attached (see section 8.3 for Pump Head Setup details). -
Page 16: Figure 2. Concentration Mode (C. Mode) Setup Configuration
User Guide KrosFlo® KR2i Real-Time Process Management (RPM™) System 1. Any combination of Auxiliary Components is possible in Manual Mode—the only required component is the TFF system pump itself. Diagram above is a full setup with all Auxiliary Components. 2. Auxiliary Scales Connect up to two scales to the Auxiliary Component Cable’s “Feed Scale”... -
Page 17: Figure 3. C/D And C/D/C Mode Setup Configuration
User Guide KrosFlo® KR2i Real-Time Process Management (RPM™) System Figure 3. C/D and C/D/C Mode setup configuration Diafiltration Buffer Reservoir Module Permeate Reservoir Feed Reservoir Diafiltration Pump Feed VPT Device KR2i Permeate Pump Permeate (Auxiliary Pump 1) Scale (FlowVPX) Pump (Auxiliary Pump 2) Scale 1. -
Page 18: Figure 5. Cfc Mode Setup Configuration
User Guide KrosFlo® KR2i Real-Time Process Management (RPM™) System Auxiliary Pump 1 will function as Diafiltration Pump 1, and the Auxiliary Pump 2 will function as Diafiltration Pump 2. Auxiliary Scales Connect first scale to the Auxiliary Component Cable’s “Feed Scale” port and second scale to the Auxiliary Component Cable’s “Permeate Scale”... -
Page 19: Figure 6. Cf/D/C Mode Setup Configuration
User Guide KrosFlo® KR2i Real-Time Process Management (RPM™) System 7.7 CF/D/C Mode Setup Figure 6. CF/D/C Mode setup configuration Module Permeate Reservoir Feed Reservoir Extra Feed Reservoir VPT Device (FlowVPX) Constant KR2i Pump Permeate Feed Scale Scale Diafiltration Pump Diafiltration Buffer (Auxiliary Pump 2) Reservoir 7.8 System Setup Using KrosFlo RPM Software Pump Control Window... - Page 20 User Guide KrosFlo® KR2i Real-Time Process Management (RPM™) System 1. To enable an alarm, highlight the alarm value and click Enter. 2. Use the directional keys to set the desired alarm value, then click Enter to save the setting. 3. To disable an alarm, change the value to 0 and TFF system will read the alarm as “OFF”. 7.10 Tubing Calibration Note: Condition tubing by running at ½...
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Page 21: Table 12. Acr2-H3I-01N Typical Flow, Pressure, And Vacuum Data - 3 Roller Pumps
User Guide KrosFlo® KR2i Real-Time Process Management (RPM™) System 8. Pump Head Setup and Operation 8.1 Introduction The Easy-Load and High-Performance Pump Heads are designed to be used with the TFF systems as a simple peristaltic pump system. The Pump Heads accept different tubing sizes for a wide range of flow rates, and the unique designs and automatic tubing retention allow for quick tubing changes. -
Page 22: Figure 7. Attaching Mounting Plate To Drive
User Guide KrosFlo® KR2i Real-Time Process Management (RPM™) System Figure 7. Attaching mounting plate to drive (4) Screws Bayonet Tabs Mounting Plate 2. Orient the pump head with its back facing the drive and insert the tang on the pump head shaft into the shaft’s slot on the drive. -
Page 23: Figure 10. Bayonet Feature Locked In Horizontal Pump Orientation
User Guide KrosFlo® KR2i Real-Time Process Management (RPM™) System Figure 10. Bayonet feature locked in horizontal pump orientation 3. Remove the pump head from the drive by holding the bayonet lock lever forward while rotating the pump head as far as possible in the counterclockwise direction, then pull the pump head away from the drive to detach it. -
Page 24: Figure 12. Pump Head In Fully Open Position
User Guide KrosFlo® KR2i Real-Time Process Management (RPM™) System Figure 12. Pump head in fully open position Actuator Lever in Far Occlusion Bed Left Position Roller 2. Before unloading tubing from the pump head, first turn off the drive. Open the pump head by moving the actuator lever counterclockwise (left), as described above. -
Page 25: Figure 14. Preparation To Mount A Second Pump Head
User Guide KrosFlo® KR2i Real-Time Process Management (RPM™) System Figure 14. Preparation to mount a second pump head Bayonet Tabs on Pump Front Cosmetic Cover Notch (here and on opposite edge) Figure 15. Engaging bayonet of second pump head to bayonet tabs on first pump head Figure 16. -
Page 26: Connecting The Flowvpx Head To The Flowvpx Standard Mount
User Guide KrosFlo® KR2i Real-Time Process Management (RPM™) System 9. FlowVPX Instrument Setup Figure 17. CTech FlowVPX System: Body 9.1 Installation and Basic Setup 9.1.1 Connecting the FlowVPX Head to the FlowVPX Standard Mount 1. Turn the FlowVPX Head on its face (logo side down) so that the two mounting holes are facing up (see Figure 18). Figure 18. -
Page 27: Figure 19. Standard Mount Clamp On Flowvpx Head
User Guide KrosFlo® KR2i Real-Time Process Management (RPM™) System Figure 19. Standard Mount Clamp on FlowVPX Head 3. Insert the mount clamp screws. Tighten with the provided 5 mm ball end driver (Figure 20). Figure 20. 5 mm Ball End Driver 4. -
Page 28: Figure 23. Tighten Mounting Post Clamp Handle
User Guide KrosFlo® KR2i Real-Time Process Management (RPM™) System 6. Ensure the mounting post on the Standard Mount is fully vertical. Tighten the Mounting Post Clamp Handle, if not already tightened (Figure 23). Figure 23. Tighten Mounting Post Clamp Handle 7. -
Page 29: Connecting The Flowvpx Instrument To The Cary 60 Spectrophotometer And Computer
User Guide KrosFlo® KR2i Real-Time Process Management (RPM™) System Note: The clamp handles can change orientation by pulling out and rotating. This allows them to be repositioned without obstruction. Figure 26. Tighten Standard Mount Clamp Handle (Left) and Repositioning the Clamp Handle (Right) Note: The clamp handles can change orientation by pulling out and rotating. -
Page 30: Figure 29. Detector Cable In Sample Compartment
User Guide KrosFlo® KR2i Real-Time Process Management (RPM™) System Figure 28. Detector Cable in sample compartment 3. Connect the Cary 60 power cable (supplied with the Cary 60) to the back of the Cary 60. Then connect the plug to an approved outlet (see Figure 30). -
Page 31: Figure 32. Flowvpx I/O Cable Connections
User Guide KrosFlo® KR2i Real-Time Process Management (RPM™) System 6. Connect the FlowVPX Power and I/O Splitter Cable (EC0208) to the top of the FlowVPX Head. The FlowVPX Power Extender Cable (EC0205) may be installed between EC0205 and EC0208 as needed. Figure 31. -
Page 32: Table 14. Acr2-H4I-01N Typical Flow, Pressure, And Vacuum Data - 3 Roller Pumps
User Guide KrosFlo® KR2i Real-Time Process Management (RPM™) System Figure 33. I/O External Cable (EC0214) to FlowVPX Head Table 14. ACR2-H4I-01N Typical Flow, Pressure, and Vacuum Data – 3 roller pumps Pin No. Wire Color Function Pin No. Wire Color Function White NC*/+24 VDC... -
Page 33: Loading The Flow Cell
User Guide KrosFlo® KR2i Real-Time Process Management (RPM™) System Figure 35. FlowVPX USB cable (EC0207) to computer 11. Plug the FlowVPX country-specific power cable into an approved outlet. 9.2 Loading and Unloading the Flow Cell 9.2.1 Loading the Flow Cell 1. - Page 34 User Guide KrosFlo® KR2i Real-Time Process Management (RPM™) System 4. Click “Connect” to establish communication between KrosFlo RPM and the VPT Device. 5. After clicking “Connect,” ensure that the serial number from the VPT device matches the serial number found by KrosFlo RPM Software (VPT Device Serial Number).
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Page 35: Unloading The Flow Cell
User Guide KrosFlo® KR2i Real-Time Process Management (RPM™) System 7. Click “Start” when the “Loading Flow Cell” pop-up window appears. 8. Follow the onscreen instructions and animations to load a flow cell. 9. Upon completing the loading of the flow cell, the pop-up window will automatically close and return to the previous VPT Device menu. - Page 36 Device menu. The serial number and flow cell name will be removed automatically. 9.3 Software/Firmware Operation VPT Devices operate through TFF System firmware and software; please contact Repligen Customer Service to ensure latest firmware and software combination has been loaded on TFF System and PC prior to operation.
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Page 37: Installation
User Guide KrosFlo® KR2i Real-Time Process Management (RPM™) System 10. Auxiliary Component Setup and Operation 10.1 Auxiliary Scales The TFF systems interface with digital Ultra Precision Surface Acoustic Wave (SAW) auxiliary scales. These SAW scales are dynamic instruments suitable for various TFF processes. For detailed information on the SAW scales, refer to the manufacturer's manual provided with the scale. -
Page 38: Auxiliary Pump Settings
User Guide KrosFlo® KR2i Real-Time Process Management (RPM™) System 5. Remote Control On (“I” icon) Note: The controls below are only accessible when the auxiliary pumps are in Internal control mode. When in External control mode, the auxiliary pumps are controlled by input from the TFF system. Table 15. -
Page 39: System Configuration
User Guide KrosFlo® KR2i Real-Time Process Management (RPM™) System b. Set-point: What pressure set-point the Valve will attempt to reach and maintain Tubing Size: Used to determine pinch distance d. Control: Set whether Valve is controlling Feed, Permeate, Retentate, or TMP pressure Start Position: The initial Start position before adjusting pinch distance: •... -
Page 40: Maintenance
User Guide KrosFlo® KR2i Real-Time Process Management (RPM™) System Figure 39. Front of KONDUiT 3. Assemble TFF flowpath and place Cond/Temp and/or UV in-line sensors at the correct position in the flowpath; see sensor descriptions below for details. Conductivity Sensor The sensors can be placed in either the permeate line or the recirculation line when used for the Diafiltration end point control. - Page 41 Although there are other methods for driving the separation process, Repligen’s hollow fiber TFF modules are designed for pressure-driven applications.
- Page 42 User Guide KrosFlo® KR2i Real-Time Process Management (RPM™) System washed through the process volume—the TFF system will know that the process volume has been washed the correct number of times. DOC0304 eRev. [Major Version]1.01.0 8/9/2023...
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Page 43: Table 16. Troubleshooting
Sensor not full of proper liquid (fixed pathlength sensors for process. UV sensor should be zeroed with the auto modes) same buffer sample is in. For further Technical Assistance, please contact Repligen at analytics-support@repligen.com or (908) 707-1009. DOC0304 eRev. [Major... -
Page 44: Table 17. Replacement And Auxiliary Parts Information
User Guide KrosFlo® KR2i Real-Time Process Management (RPM™) System 13. Replacement and Auxiliary Parts More information regarding the KrosFlo KR2i RPM System can be found by visiting https://www.repligen.com/products/analytics/rpm-system Table 17. Replacement and Auxiliary Parts Information Description Part number Auxiliary Pumps... - Page 45 User Guide KrosFlo® KR2i Real-Time Process Management (RPM™) System Stir Plates Digital Magnetic Stirrer, 1000 RPM, 120/100 V (FS-500 Recommended Use) ACFS-SP500-120 Digital Magnetic Stirrer, 1000 RPM, 230/100 V (FS-500 Recommended Use) ACFS-SP500-230 Fittings Kit MicroKros/MidiKros Fittings Kit ACPX-CD MidiKros TC Fittings Kit ACPX-T-01N MiniKros Sampler Fittings Kit ACPX-S-01N...
- Page 46 User Guide KrosFlo® KR2i Real-Time Process Management (RPM™) System PharmaPure Tubing Pack, 25', 0.19" ID, 0.375"OD, #15, 3/16" Hose Barb ACTU-P15-25N PharmaPure Tubing Pack, 25', 0.12" ID, 0.25"OD, #16, 1/8" Hose Barb ACTU-P16-25N PharmaPure Tubing Pack, 25', 0.25" ID, 0.375"OD, #17, 1/4" Hose Barb ACTU-P17-25N PharmaPure Tubing Pack, 25', 0.31"...
- Page 47 Repligen Corporation 685 Route 202/206 Bridgewater, NJ 08807 analytics-support@repligen.com (908) 707-1009 DOC0304 eRev. [Major Version]1.01.0 [Publication repligen.com © 2023 Repligen Corporation. All rights reserved. The trademarks mentioned herein are the property of Repligen Corporation and/or its affiliate(s) or their respective owners.
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