REPLIGEN CTech SoloVPE PLUS User Manual page 40

CTech™ SoloVPE® PLUS System User Guide
Glossary
There are many terms, definitions, and naming conventions associated with your SoloVPE PLUS System that are helpful to know and
understand. This section of the manual provides an overview of these concepts and serves as a quick reference.
This glossary contains content developed by Repligen subject-matter experts or retrieved (whole or in part) from the American
Society for Quality glossary, the International Accreditation Forum website, and Spectroscopy magazine's "The Molecular
Spectroscopy Terminology Guide" online.
Averaging Time: The Cary Spectrophotometer setting that configures how long the system will collect data at each wavelength. Able
to be configured in seconds, this number corresponds to many pulses of the Xenon flash lamp. One pulse corresponds to 1/80th of a
second or 0.0125 seconds; therefore, each second is 80 pulses of the lamp.
Beer-Lambert Law: The law of physics that describes the proportional relationship between absorbance, pathlength, and
concentration.
Delivery Fiber: The fiber-optic cable that carries light from the Cary spectrophotometer to the variable pathlength device.
Detector: A device used to detect light being transmitted through the sample.
Detector Cable: This component sends absorbance information from the VPT instrument to the Cary 60 to be analyzed and
displayed for the user.
Detector Window: The transparent, protective cover of the VPT instrument's Detector.
Extinction Coefficient (EC): An intrinsic property of a substance, described by a numerical value that quantifies the propensity of a
substance to absorb electromagnetic radiation at a specific wavelength. It is one of the terms used in Beer-Lambert law (ԑ).
Fibrette Optical Component: The component of the SoloVPE PLUS System that delivers the light from the Delivery Fiber to the
sample. It allows the measurement pathlength to be changed based upon its vertical motion within the sample.
Fixed Zero: The position the system drives down to prepare the SoloVPE PLUS and Fibrette Optical Component for data acquisition.
When in this position, the Fibrette tip is in contact with the bottom of the sample vessel. All pathlength parameters and motion are
referenced from this position, which corresponds to a pathlength of zero millimeters. Also referred to as Zero Position and Zero
Pathlength.
Home Position: This is the fully raised position of the SoloVPE PLUS System. This allows maximum access to the sample vessel,
sample vessel holder, and the Fibrette Coupler. It is the position the SoloVPE PLUS needs to be in for loading and unloading of
Fibrette Optical Component and Fibrette Coupler.
Insert Holder: The component of the SoloVPE PLUS System that holds the Quick Set Fibrette Coupler Insert, connecting it to the
Translation Stage.
Pathlength: The distance the measured light travels through the sample when making absorbance spectroscopy measurements
based upon the Beer-Lambert law. In the variable pathlength system, this distance, generally expressed in millimeters, is defined by
the physical gap between the bottom of the sample vessel containing the solution and the tip of the Fibrette Optical Component
that is submerged in the sample.
Quick Set Fibrette Coupler: The current Fibrette Coupler design. Comprising the stainless-steel Insert and the black-plastic Insert
Holder, it allows consistent positioning of the Fibrette Optical Component.
Quick Set Fibrette Coupler Insert: The stainless steel Fibrette holder. A replaceable, disposable part to be used with the Quick Set
Fibrette Coupler.
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DOC0362 eRev. 1.0 [Publication Date]