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TangenX SC Series
User Manuals: REPLIGEN TangenX SC Series TFF Device
Manuals and User Guides for REPLIGEN TangenX SC Series TFF Device. We have
1
REPLIGEN TangenX SC Series TFF Device manual available for free PDF download: User Manual
REPLIGEN TangenX SC Series User Manual (31 pages)
TFF Device
Brand:
REPLIGEN
| Category:
Laboratory Equipment
| Size: 4 MB
Table of Contents
Table of Contents
3
1 About this Document
5
2 Introduction
6
Additional References
6
3 Device Components
8
Figure 1. Tangenx SC TFF Device (Side View and Top View)
8
Recommended Device Storage Conditions
9
4 Pump Requirements
9
5 Preparing to Use the Tangenx SC TFF Device
9
Required Tools and Components
9
Unpackaging the Device
10
6 System Set-Up
10
Installing the Tangenx SC TFF Device
10
Figure 2. How to Pick up the Device
10
Using Aseptiquik Genderless Connectors
11
TFF System Flow Path Parts
11
Figure 3. Using Aseptiquik Genderless Connectors
11
Figure 4. System Configuration Without Tangenx SC TFF Device
12
Connecting the Tangenx SC TFF Device
13
Figure 5. Connecting Tangenx SC TFF Device to Tube Sets
13
Estimate Hold-Up Volume
14
Figure 6. System Configuration with All Pinch Clamps Closed
14
Device Flush
15
Flush the Tangenx SC TFF Device Retentate Flow Path
15
Figure 7. System Configuration Showing Flow Path for Hold-Up Volume
15
Figure 8. System Configuration for Retentate Port WFI Flush
16
Flush the Tangenx SC TFF Device Permeate Flow Path
17
Figure 9. System Configuration for Permeate Port WFI Flush
17
Air Integrity Test
18
7 Process Execution
18
Buffer Equilibration
18
Equilibrate the Tangenx SC TFF Device Retentate Flow Path
18
Equilibrate the Tangenx SC TFF Device Permeate Flow Path
19
Figure 10. System Configuration for Retentate Outlet Port Buffer Equilibration
19
Product Introduction
20
Figure 11. System Configuration for Filtration Device Permeate Outlet Port Buffer Equilibration
20
Figure 12. System Configuration for Product Introduction
21
Optimization
22
Optimization through Flux Excursion
22
Figure 13. System Configuration for Recirculation Mode
22
Figure 14. System Configuration for Optimization Studies
23
Process Optimization Data Plotting and Analysis
24
Concentration
24
Determination of Concentration Factor (CF)
24
Figure 15. TMP Vs LMH at 3 Different Crossflow Rates
24
Executing Concentration Step
25
Figure 16. System Configuration for Product Concentration
25
Diafiltration
26
Prepare for Diafiltration
26
Execute Diafiltration
26
Figure 17. Relationship between Remaining Contaminant and Diavolumes
26
Stop the Diafiltration Process
27
Figure 18. System Configuration for Diafiltration
27
Product Recovery
28
Figure 19. System Configuration for Stopping Diafiltration
28
Figure 20. System Configuration for Product Recovery, Step 2
29
Inactivation and Disposal
30
Figure 21. System Configuration for Product Recovery, Step 3
30
8 Index
31
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