REPLIGEN CTech FlowVPE Maintenance Manual

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CTech™ FlowVPE® System
CTech™ FlowVPE 10 mm GxP Flow Cell Loading Procedure

Introduction

The purpose of this document is to become familiar and comfortable with handling the various components of the
GxP Flow Cell as well as correctly following the loading and unloading procedures. The following instruction details
these procedures, as well as the setup required for the FlowVPE hardware prior to the loading procedure.
These instructions must be followed very closely, as they ensure proper loading and operation of the
FlowVPE System. Failure to do so may result in damage to the Flow hardware.
The User is the Critical Link Between the Software and Hardware:
The user must tell the software when the Flow Cell is mounted and demounted. The
software is set up to prompt accordingly, but the user must be aware of the current state
of the hardware for safe and effective use of the FlowVPE System.

How to Load the GxP Flow Cell and Fibrette

1. Click on Load Controls in the FlowVPE sidebar. The Flow Head moves to the loading position and opens the Load
Controls window.
1 | analytics-applications@repligen.com | +1 908-707-1009 | ctech.repligen.com
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WARNING
Figure 1. Load Controls button.
Maintenance Guide
DOC0093 Rev. 02 11/12/2021
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Summary of Contents for REPLIGEN CTech FlowVPE

  • Page 1 How to Load the GxP Flow Cell and Fibrette 1. Click on Load Controls in the FlowVPE sidebar. The Flow Head moves to the loading position and opens the Load Controls window. Figure 1. Load Controls button. 1 | analytics-applications@repligen.com | +1 908-707-1009 | ctech.repligen.com...
  • Page 2 Flow Cell are engaged with the Detector Module. For assistance in mounting the Flow Cell properly, refer to General Troubleshooting in the FlowVPE User Manual DOC0096. 2 | analytics-applications@repligen.com | +1 908-707-1009 | ctech.repligen.com...
  • Page 3 Detector Module. The Fibrette will slide into the Fibrette Clamp. Figure 5. Flow Cell mounting to Flow Head. 6. Secure the Flow Cell into the Detector Module using the four captive fasteners. Figure 6. Securing Flow Cell to Detector Module. 3 | analytics-applications@repligen.com | +1 908-707-1009 | ctech.repligen.com...
  • Page 4 10. Swing the Clamp Lock Arm all the way to the right and seat the thumb nut into the mating counter bore. Figure 8. Locking Fibrette Clamp position. 11. Tighten the thumb nut into the mating counter bore until it makes a hard stop. 4 | analytics-applications@repligen.com | +1 908-707-1009 | ctech.repligen.com...
  • Page 5 13. Insert the tan portion of the Delivery Fiber into the SC-ST adapter so the small spline on the tan Delivery Fiber connecter lines up with the channel in the adapter. Figure 10. Delivery Fiber connected to Flow Fibrette. 5 | analytics-applications@repligen.com | +1 908-707-1009 | ctech.repligen.com...
  • Page 6 14. Close the cover door. When the Flow Cell and Flow Fibrette have been mounted onto the Flow Head properly, the FlowVPE instrument will appear as shown in Figure 11. Continue the steps to finalize the setup process. Figure 11. FlowVPE instrument with mounted Flow Cell. 6 | analytics-applications@repligen.com | +1 908-707-1009 | ctech.repligen.com...
  • Page 7 16. The Load Status in the center of the Load/Unload Controls box will turn blue and will show the Load Status as LOADED to confirm that the Flow Cell is loaded. Figure 13. LOADED Status in Cell Load/Unload Controls window. Congratulations, you have now successfully loaded the Flow Cell! 7 | analytics-applications@repligen.com | +1 908-707-1009 | ctech.repligen.com...
  • Page 8 9. In the FlowVPE software, click on Unload in the Load/Unload Controls window. 10. Follow prompts in the software to fully unload the Flow Cell. 11. The Load Status in the Load/Unload Controls window should now read UNLOADED. 8 | analytics-applications@repligen.com | +1 908-707-1009 | ctech.repligen.com...

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