Mixing Parameters; Flow Rate Ratio; Total Volume - cytiva NanoAssemblr Ignite Operating Instructions Manual

5.1.2

Mixing parameters

Introduction
Mixing parameters can influence characteristics such as particle size and polydisper-
sity index (PDI). Particle size and PDI are also influenced by the chemistry, including the
choice of carrier, choice of solvents and buffers, and concentrations of reagents.
Mixing parameters include the Flow rate ratio, the Total volume and the Total flow
rate . NxGen Dilution and NxGen 500D formulations also require a Dilution ratio.
The total flow rate and the flow rate ratio are the most influential mixing parameters to
tune for achieving optimal particles for a given application. For more information on
parameter combinations, including detailed application notes, papers, webinars, and
other resources, visit cytiva.com.
Tip:

Flow rate ratio

The flow rate ratio is the ratio between the volumes of fluids from the C and R syringes.
For example, in a nanoprecipitation reaction, the flow rate ratio specifies the ratio
between the polar aqueous phase and the organic phase. Increasing the proportion of
the aqueous phase increases the magnitude of the polarity change experienced by the
molecules. In many formulations, an increase in the aqueous phase leads to smaller
particles.
The table below gives the range of values that can be input for the parameter Flow
rate ratio.
Instrument
Ignite
Ignite+

Total volume

The value of the parameter Total volume gives the amount of liquid that is formulated
before dilution during the procedure. The table below gives the range of possible
values.
Instrument
Ignite
Ignite+
NanoAssemblr Ignite and Ignite+ Operating Instructions NIN1134 AA
Adjust the total flow rate and the flow rate ratio and then evaluate the
formulation composition to determine the optimal parameter combination.
Flow rate ratio
1:1 to 10:1
Total volume
1 mL to 20 mL
1 mL to 60 mL
5 Preparation
5.1 Parameters
5.1.2 Mixing parameters
59