cytiva AKTA flux s Operating Instructions Manual
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cytiva AKTA flux s Operating Instructions Manual

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Operating Instructions
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ÄKTA flux
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  • Page 1 ÄKTA flux s Operating Instructions Original instructions ÄKTA flux cytiva.com...

  • Page 2: Table Of Contents

    Table of Contents Table of Contents Introduction ......................5 Important user information ........................6 About this manual ............................7 Associated documentation ........................8 Additional user documentation - additional/specific documentation ........10 Abbreviations ..............................11 Safety instructions ..................... 13 Safety precautions ............................14 Labels .................................
  • Page 3 Table of Contents 6.1.2 Install hollow fiber cartridges ........................102 Install Kvick Lab packet and holder ......................106 Testing filters ..............................108 Calibration ................................ 109 6.4.1 Calibration of the feed pump ........................110 6.4.2 Calibration of the transfer pump ......................115 6.4.3 Calibration of the permeate pump ......................
  • Page 4 Table of Contents 11.6.7 Other regulations and standards ......................203 11.7 Ordering information ........................... 204 11.8 Health and Safety Declaration Form ...................... 205 Index ........................... 207 ÄKTA flux s Operating Instructions 29046893 AG...

  • Page 5: Introduction

    1 Introduction Introduction About this chapter This chapter contains important user information, descriptions of safety notices, regu- latory information, and intended use of ÄKTA™ flux s. In this chapter Section See page Important user information About this manual Associated documentation Additional user documentation - additional/specific docu- mentation Abbreviations...

  • Page 6: Important User Information

    1 Introduction 1.1 Important user information Important user information Read this before operating the product All users must read the entire Operating Instructions before installing, oper- ating or maintaining the product. Always keep the Operating Instructions at hand when operating the product. Do not operate the product in any other way than described in the user documenta- tion.

  • Page 7: About This Manual

    1 Introduction 1.2 About this manual About this manual Purpose of this manual The Operating Instructions provide you with the information needed to install, operate, and maintain the product in a safe way. Scope of this manual This Operating Instruction manual is valid for ÄKTA flux s. ÄKTA flux s is also referred to as the system, or the product in this document.

  • Page 8: Associated Documentation

    Introduction This section describes the user documentation that is delivered with the product, and how to find related literature that can be downloaded or ordered from Cytiva. User documentation for ÄKTA flux s The user documentation is listed in the table below.
  • Page 9 1 Introduction 1.3 Associated documentation Documentation Main contents ÄKTA flux s Instrument Display help Brief descriptions of the screens and functions in the Instrument Display software. to access the Instrument Display help. ÄKTA flux s Workstation software Help Overview and detailed descriptions of menus and functions in the Workstation software.

  • Page 10: Additional User Documentation - Additional/Specific Documentation

    List of spare parts available from Cytiva. Functional Specification Software function description. Component documentation Documentation for components produced both by Cytiva and by a third-party are, if existent, also included in the Product Documentation. ÄKTA flux s Operating Instructions 29046893 AG...

  • Page 11: Abbreviations

    1 Introduction 1.5 Abbreviations Abbreviations Introduction This section explains abbreviations that appear in the user documentation for ÄKTA™ flux s ÄKTA flux s. Abbreviations Abbreviation Definition (English) Translation (local language) aseptic connection device aseptic connection device controlled environment module controlled environment module controlled variable controlled variable The output from the PID controller, or...
  • Page 12 1 Introduction 1.5 Abbreviations Abbreviation Definition (English) Translation (local language) setpoint setpoint The target value that an automatic The target value that an automatic control system, for example PID control system, for example PID controller, will attempt to reach by controller, will attempt to reach by adjusting the process control inputs.

  • Page 13: Safety Instructions

    2 Safety instructions Safety instructions About this chapter This chapter describes safety precautions, labels and symbols that are attached to the equipment. In addition, the chapter describes emergency and recovery procedures, and provides recycling information. Important WARNING Before installing, operating or maintaining the product, all users must read and understand the entire contents of this chapter to become aware of the hazards involved.

  • Page 14: Safety Precautions

    2 Safety instructions 2.1 Safety precautions Safety precautions Introduction ÄKTA flux s is powered by mains voltage and handles materials that might be hazardous. Before installing, operating or maintaining the system, you must be aware of the hazards described in this manual. The safety precautions in this section are grouped into the following categories: •...

  • Page 15: General Precautions

    2 Safety instructions 2.1 Safety precautions General precautions WARNING Operation and user maintenance of ÄKTA flux s must be performed by properly trained personnel only. WARNING Do not operate the product in any other way than described in the user documentation. WARNING Risk assessment.
  • Page 16 2 Safety instructions 2.1 Safety precautions WARNING When using hazardous chemical and biological agents, take all suitable protective measures, such as wearing protective glasses and gloves resistant to the substances used. Follow local and/or national regulations for safe operation and maintenance of ÄKTA flux s.
  • Page 17 2 Safety instructions 2.1 Safety precautions WARNING Some of the chemicals used with the columns may be flammable under certain conditions. Make sure to use chemicals only under conditions where they are not flammable. Refer to local and/or national classifications of flammable liquids. Installing and moving the system CAUTION Before moving ÄKTA flux s the following must be done:...
  • Page 18 Make sure your fingers not are pinched under ÄKTA flux s when the instrument is placed onto a bench. CAUTION Use only tubing supplied by Cytiva. CAUTION Avoid that the filter falls when installing filters. • Handle filter with care.

  • Page 19: Power Supply

    WARNING ÄKTA flux s must always be connected to a grounded power outlet. WARNING Only use grounded power cords delivered or approved by Cytiva. WARNING Always disconnect power to ÄKTA flux s before replacing fuses. ÄKTA flux s Operating Instructions 29046893 AG...
  • Page 20 2 Safety instructions 2.1 Safety precautions Operation WARNING Do not use the product if it is not working properly, or if it has suffered any damage, for example: • damage to the power cord or its plug • damage caused by dropping the equipment •...

  • Page 21: Maintenance

    Make sure the control valve is released when there is no active filtration. Maintenance WARNING All repairs should be done by service personnel authorized by Cytiva. Do not open any covers or replace parts unless specifically stated in this operating instruction. ÄKTA flux s Operating Instructions 29046893 AG...
  • Page 22 WARNING Use only approved parts. Only spare parts and accessories that are approved or supplied by Cytiva may be used for maintaining or servicing the product. WARNING When using hazardous chemicals, run System CIP and Membrane CIP to flush the entire system, including filters, with distilled water until a pH of approximately pH 7.0 is reached, before...
  • Page 23 • Maintenance and pump tubing replacement of the ÄKTA flux s must be scheduled on regular basis with a Cytiva representative and performed by properly trained personnel only. • Always use appropriate Personal Protective Equipment (PPE) when operating and interacting with ÄKTA flux s.

  • Page 24: Labels

    2 Safety instructions 2.2 Labels Labels Introduction This section describes the system label and other safety or regulatory labels that are attached to the product. System label The system label is located on the back of the equipment. The system label identifies the equipment and shows electrical data, regulatory compliance, and warning symbols.
  • Page 25 2 Safety instructions 2.2 Labels Safety label The warning label on the pumps indicates that the fingers must be kept away from the moving rotor. ÄKTA flux s Operating Instructions 29046893 AG...

  • Page 26: Emergency Procedures

    2 Safety instructions 2.3 Emergency procedures Emergency procedures Introduction This section describes how to do an emergency shutdown of ÄKTA flux s, and the result in the event of power failure. The section also describes how to restart the system after emergency shut down or power failure.
  • Page 27 2 Safety instructions 2.3 Emergency procedures Step Action If the power has been lost: switch on power to ÄKTA flux s by turning on the power switch to the I position. Result: The system computer starts automatically. Restart the process. ÄKTA flux s Operating Instructions 29046893 AG...

  • Page 28: System Description

    3 System description System description About this chapter This chapter provides an overview of the technical properties of ÄKTA flux s. In this chapter Section See page Illustrations of ÄKTA flux s System control Standard equipment Optional equipment Filter devices Flowchart ÄKTA flux s Operating Instructions 29046893 AG...

  • Page 29: Illustrations Of Äkta Flux S

    3 System description 3.1 Illustrations of ÄKTA flux s Illustrations of ÄKTA flux s Front view with all options Part Function Transfer pump (optional) Tank holder with tank balance and motor for mixer Tank ÄKTA flux s Operating Instructions 29046893 AG...
  • Page 30 3 System description 3.1 Illustrations of ÄKTA flux s Part Function Air filter Operator touchscreen Upper drain valve Retentate pressure sensor Pr Filter holder for Kvick Lab™ packet shown (optional) Retentate pressure control valve Feed pressure sensor Pf and temperature sensor Lower drain valve Power switch Feed pump...
  • Page 31 3 System description 3.1 Illustrations of ÄKTA flux s Rear view with all options Part Function Permeate pressure control valve Permeate pressure sensor Pp (optional) Permeate pump (optional) USB-connector protection Ventilation holes for cabinet cooling fan Air inlet Circuit breakers Power cord connection and fuse drawer ÄKTA flux s Operating Instructions 29046893 AG...

  • Page 32: System Control

    3 System description 3.2 System control System control Introduction ÄKTA flux s is controlled by a process and control software from a built-in computer. The computer starts automatically when the system power is turned on. Operator touchscreen The user interacts with the system from the touchscreen. The touchscreen shows: •...
  • Page 33 3 System description 3.2 System control Part Description Function Settings button Submenus: USB Device, Alarms, Configure, Calibration and System Information. Information panel Displays active automated functions and the settings for these. Start and Stop Data This function is active when a USB device is Logging connected.
  • Page 34 3 System description 3.2 System control Main screen with no optional pumps installed If the optional pumps not are installed, they are gray on the main screen. The Lock button on the tank level control is dimmed and not possible to use. ÄKTA flux s Operating Instructions 29046893 AG...
  • Page 35 3 System description 3.2 System control Control panel Part Description Function Transfer Adjusts the transfer pump (optional) and displays transfer pump rotation speed or flow rate. GE Title or job number 3/25/2014 Level Adjusts level control and displays amount of fluid in the tank.
  • Page 36 3 System description 3.2 System control Buttons The control panel includes the following command buttons: Button Description A Start button with surrounding blue circle indicates that the parameter Stop Start is not active. A Stop button with a surrounding green circle indicates that the param- Start Stop Start...
  • Page 37 3 System description 3.2 System control Process flowchart panel Part Function Transfer pump (optional) Mixer and tank Feed pump Feed pressure sensor (Pf) Temperature sensor Retentate pressure sensor (Pr) Filter Permeate pump (optional) Permeate pressure sensor (Pp) (optional) ÄKTA flux s Operating Instructions 29046893 AG...
  • Page 38 3 System description 3.2 System control Indicators The process flowchart panel includes the following indicators: Indicator Description A blue circle around the motor symbol indicates that the pump is not running. A green circle around the motor symbol indicates that the pump is running.
  • Page 39 3 System description 3.2 System control Parameter panel Part Symbol Function Displays feed pressure Displays retentate pressure Displays permeate pressure ΔP Displays Δpressure ΔP = Pf - Pr Displays transmembrane pressure TMP = [(Pf + Pr)/2] - Pp Displays system temperature Calculated permeate flow Stopping the process Button...
  • Page 40 3 System description 3.2 System control Button Function The pumps can be stopped at any time by tapping the Stop Pumps button on the main screen. Stop All running pumps on the system will be stopped. A Start red circle will be shown on the Start button. Use the Stop Pumps button if the pumps needs to Start be stopped quickly during an unexpected occur-...

  • Page 41: Standard Equipment

    3 System description 3.3 Standard equipment Standard equipment Introduction This section provides an overview of the standard components of ÄKTA flux s. In this section Section See page 3.3.1 Feed pump, pressure sensors and pressure control valve 3.3.2 Tank 3.3.3 Communication connection ÄKTA flux s Operating Instructions 29046893 AG...

  • Page 42: Feed Pump, Pressure Sensors And Pressure Control Valve

    3 System description 3.3 Standard equipment 3.3.1 Feed pump, pressure sensors and pressure control valve 3.3.1 Feed pump, pressure sensors and pressure control valve The feed pump A peristaltic feed pump used in the feed line. The optional pumps used in the transfer and permeate lines are of the same type.
  • Page 43 3 System description 3.3 Standard equipment 3.3.1 Feed pump, pressure sensors and pressure control valve Pressure sensors The feed pressure sensor, Pf, and the retentate pressure sensor, Pr, are both used in the recirculation loop, see Section 4.1 Recirculation loop, on page 55.
  • Page 44 3 System description 3.3 Standard equipment 3.3.1 Feed pump, pressure sensors and pressure control valve ÄKTA flux s Operating Instructions 29046893 AG...

  • Page 45: Tank

    3 System description 3.3 Standard equipment 3.3.2 Tank 3.3.2 Tank Illustration Part Description Air filter Check valve Tank lid Level indication Magnetic stir bar Retentate inlet Feed outlet Transfer inlet Description ÄKTA flux s has a 0.5 liter tank. The tank has a feed outlet, transfer and retentate inlets. It is equipped with a check valve and an air filter.

  • Page 46: Communication Connection

    3 System description 3.3 Standard equipment 3.3.3 Communication connection 3.3.3 Communication connection USB connection The USB flash drive is used to save process data and produce a system health report. A protective cap is used to cover the USB flash drive. ÄKTA flux s Operating Instructions 29046893 AG...

  • Page 47: Optional Equipment

    3 System description 3.4 Optional equipment Optional equipment Introduction Optional pumps can be installed to transfer liquid to the tank and to control the permeate flow. A permeate pressure sensor can also be installed in the permeate line. A holder for Kvick Lab filter is an optional item. Transfer pump An optional transfer pump is available to set up the transfer line.

  • Page 48: Filter Devices

    3 System description 3.5 Filter devices Filter devices Introduction This section contains a description and an overview of the filter types that can be installed with ÄKTA flux s: The following filter types can be used: • Hollow fiber cartridges •...

  • Page 49: Hollow Fiber Cartridges

    The permeate penetrates the lumen's wall in the fibers and flows outside the fibers and is collected through one of the side connectors. Available HF cartridges ÄKTA flux s can be used with the Cytiva Hollow Fiber Cartridges MidGee™, Hollow fiber Start AXM, and Start AXH cross flow cartridges. More information •...

  • Page 50: Kvick Lab Packet And Holder

    Kvick Lab packet and holder can be used with ÄKTA flux s. It is possible to attach up to three Kvick Lab packets to the holder. Inlet and outlet filter tubing in correct dimen- sions and lengths can be ordered separately from Cytiva. Illustration...
  • Page 51 • Refer to Quick Reference Guide (11000387), for more information regarding Kvick Lab packet and holder. • Contact Cytiva for more information about other filter solutions that can be used with ÄKTA flux s filtration systems. ÄKTA flux s Operating Instructions 29046893 AG...

  • Page 52: Flowchart

    3 System description 3.6 Flowchart Flowchart Introduction This section contains a flowchart for ÄKTA flux s. The process components and pres- sure sensors are listed. Flowchart illustration Black: standard components; Red: optional components Process components The following table lists the process components that are shown in the flowchart. Function Feed pump Lower drain valve...
  • Page 53 3 System description 3.6 Flowchart Function Upper drain valve Retentate inlet Tank balance Air filter Tank Mixer Feed outlet from tank Tank transfer inlet Transfer pump (optional) External tank connection Filter feed inlet Filter retentate outlet Filter permeate outlet Permeate pressure sensor Pp (optional) Permeate pump (optional) Permeate outlet ÄKTA flux s Operating Instructions 29046893 AG...

  • Page 54: Flow Lines

    4 Flow lines Flow lines About this chapter This chapter provides an overview of the flow lines in ÄKTA flux s. In this chapter Section See page Recirculation loop Permeate line Transfer line ÄKTA flux s Operating Instructions 29046893 AG...

  • Page 55: Recirculation Loop

    4 Flow lines 4.1 Recirculation loop Recirculation loop Introduction The recirculation loop consists of the feed line and the retentate line. Illustration Part Function Feed outlet from tank Feed pump Lower drain valve Feed pressure sensor Pf and temperature sensor Feed inlet to filter Retentate outlet from filter Retentate pressure sensor Pr...
  • Page 56 4 Flow lines 4.1 Recirculation loop Part Function Retentate inlet to tank Flow path The feed line transfers liquid from the tank to the filter via the feed pump. The retentate with particles or molecules too large to pass through the filter pores is returned to the tank via the retentate line.
  • Page 57 4 Flow lines 4.1 Recirculation loop Transmembrane pressure Formula: TMP = [(Pf+ Pr)/2 - Pp] where under ultrafiltration Pp is 0 bar Pf, Pr, and Δp are dependent upon filter and feed flow Pf can be adjusted via controlling the feed flow or the back pressure in the recirculation line via the retentate pressure control valve placed after the filter.

  • Page 58: Permeate Line

    4 Flow lines 4.2 Permeate line Permeate line Introduction The permeate line carries the permeate that exits the filter through the permeate outlet. Permeate flow control Liquid containing particles or molecules small enough to pass through the filter pores is collected as permeate. Permeate exits the filter through the permeate outlet on the filter.
  • Page 59 4 Flow lines 4.2 Permeate line Optional permeate equipment The permeate line can be equipped with a permeate pump and a pressure sensor. The permeate pump is used to control the permeate flow rate and the permeate pressure sensor, Pp, measures permeate pressure. Part Function Permeate pressure sensor inlet...

  • Page 60: Transfer Line

    4 Flow lines 4.3 Transfer line Transfer line Introduction The transfer line requires the optional transfer pump. Illustration Part Function Transfer pump Tubing outlet from transfer pump Transfer line Transfer inlet to tank Description The transfer line transfers liquid from an external tank to the ÄKTA flux s tank via the transfer pump.

  • Page 61: Installation

    5 Installation Installation About this chapter This chapter provides required information to enable users and service personnel to unpack, install, move and transport ÄKTA flux s. In this chapter Section See page Site requirements Unpack the ÄKTA flux s Transport Set up the ÄKTA flux s Installation test Precautions...
  • Page 62 5 Installation CAUTION Do not transport, handle and store ÄKTA flux s in any other way than described in this manual. ÄKTA flux s Operating Instructions 29046893 AG...

  • Page 63: Site Requirements

    5 Installation 5.1 Site requirements Site requirements Summary of requirements The following table gives a summary of power supply and environmental requirements. Parameter Requirement Supply voltage 100-120/220-240 V AC, ± 10% Phases Single phase (with 3P ground pole) Frequency 50 to 60 Hz Maximum power Nominally 400 W Grounding...

  • Page 64: Unpack The Äkta Flux S

    • that all equipment is enclosed in the crate according to the packing list. • the equipment for any apparent damage and document carefully if found. If any equipment is missing or damaged are found, contact Cytiva immediately. Safety instructions The following precautions must be considered when unpacking the product.
  • Page 65 5 Installation 5.2 Unpack the ÄKTA flux s CAUTION Two persons are required to lift ÄKTA flux s. Lift only in the bottom part of the system. CAUTION Make sure that the system is placed on a stable, level bench with adequate space for ventilation.

  • Page 66: Transport

    5 Installation 5.3 Transport Transport Introduction This section outlines important information that must be considered when trans- porting ÄKTA flux s. Moving ÄKTA flux s CAUTION Before moving ÄKTA flux s the following must be done: 1. Empty ÄKTA flux s. 2.

  • Page 67: Set Up The Äkta Flux S

    5 Installation 5.4 Set up the ÄKTA flux s Set up the ÄKTA flux s Introduction This section describes the steps that need to be taken to set up ÄKTA flux s before use. In this section Section See page 5.4.1 Power supply and USB connection 5.4.2...

  • Page 68: Power Supply And Usb Connection

    5 Installation 5.4 Set up the ÄKTA flux s 5.4.1 Power supply and USB connection 5.4.1 Power supply and USB connection Power supply and fuses WARNING The supply voltage must correspond to the markings on the system. The illustration below shows the location of the power supply and fuses on the ÄKTA flux s.
  • Page 69 5 Installation 5.4 Set up the ÄKTA flux s 5.4.1 Power supply and USB connection Part Function Automatic circuit breakers Connect power supply WARNING ÄKTA flux s must always be connected to a grounded power outlet. WARNING Do not block access to the power switch and power cord. The power switch must always be easy to access.
  • Page 70 5 Installation 5.4 Set up the ÄKTA flux s 5.4.1 Power supply and USB connection USB connection CAUTION Make sure that the USB port is protected from moisture and liquid by always having a protective cap on. The protective cap for the USB flash drive is placed on the right side of ÄKTA flux s seen from the front.

  • Page 71: Install And Remove The Tank

    5 Installation 5.4 Set up the ÄKTA flux s 5.4.2 Install and remove the tank 5.4.2 Install and remove the tank Precautions WARNING Use fume mask during tank opening when processing hazardous liquids, to avoid possible exposure to aerosols. CAUTION Handle tank with care.

  • Page 72: Tubing

    Always use appropriate Personal Protective Equipment (PPE) when operating and inter- acting with ÄKTA flux s. CAUTION Use only tubing supplied by Cytiva. Luer connections All tubing is connected with Luer connections. Female Luer Male Luer ÄKTA flux s Operating Instructions 29046893 AG...
  • Page 73 5 Installation 5.4 Set up the ÄKTA flux s 5.4.3 Tubing Pump tubing tags All pump tubing is tagged. The illustration below shows an example of a feed pump tubing tag. The characters on the tag are explained in the table. Location Description Dimension...
  • Page 74 5 Installation 5.4 Set up the ÄKTA flux s 5.4.3 Tubing Flow line tubing All tubing for the flow lines is tagged. The illustration below shows an example of a tubing tag. The characters on the tag are explained in the table. Loca Description tion...
  • Page 75 5 Installation 5.4 Set up the ÄKTA flux s 5.4.3 Tubing Tubing connections The illustration shows the tubing used in ÄKTA flux s. Flow line tubing The tubing listed below is delivered with ÄKTA flux s. Tubing Tubing size (ID=Inner diameter, OD=Outer diameter) Tubing from tank 3.2 mm ID ×...
  • Page 76 5 Installation 5.4 Set up the ÄKTA flux s 5.4.3 Tubing Pump tubing The pump tubing listed below can be used with ÄKTA flux s. Tubing size Feed pump Transfer Permeate pump pump 1.6 mm ID × 4.8 mm 3.2 mm ID × 6.3 mm 1.6 mm ID ×...
  • Page 77 5 Installation 5.4 Set up the ÄKTA flux s 5.4.3 Tubing Tubing for cleaning-in-place (CIP) Tubing adapted for CIP is delivered with the instrument as standard. Part Description Tubing size MM11 Tubing to permeate pressure 1.6 mm ID × 4.8 mm sensor MM12 Tubing to retentate pressure...
  • Page 78 5 Installation 5.4 Set up the ÄKTA flux s 5.4.3 Tubing Upper drain valve Use the exploded view as a help to put together the upper drain valve. ÄKTA flux s Operating Instructions 29046893 AG...
  • Page 79 5 Installation 5.4 Set up the ÄKTA flux s 5.4.3 Tubing Lower drain valve Use the exploded view as a help to put together the lower drain valve. ÄKTA flux s Operating Instructions 29046893 AG...
  • Page 80 5 Installation 5.4 Set up the ÄKTA flux s 5.4.3 Tubing Permeate line tubing The P1L tubing is used on the permeate side after the filter, when the optional permeate pressure pump is not installed. ÄKTA flux s Operating Instructions 29046893 AG...
  • Page 81 5 Installation 5.4 Set up the ÄKTA flux s 5.4.3 Tubing Transfer pump tubing The T1L tubing is used when the optional transfer pump is installed. It is connected to the tank and to the pump tubing in the transfer pump. ÄKTA flux s Operating Instructions 29046893 AG...
  • Page 82 5 Installation 5.4 Set up the ÄKTA flux s 5.4.3 Tubing Filtration tubing All filtration tubing is tagged and delivered with the instrument. The illustration below shows an example of the filter tubing tags. The characters in the tag are explained in the table.

  • Page 83: Install The Transfer Pump

    5 Installation 5.4 Set up the ÄKTA flux s 5.4.4 Install the transfer pump 5.4.4 Install the transfer pump Introduction This section describes how to install the optional transfer pump. Precautions WARNING Disconnect power to the product before installation of the optional pump.
  • Page 84 5 Installation 5.4 Set up the ÄKTA flux s 5.4.4 Install the transfer pump Install the transfer pump Follow the instructions below to install the transfer pump. Step Action Disconnect the power. Unscrew the screws holding the casing for the transfer pump connections. Remove the casing.
  • Page 85 5 Installation 5.4 Set up the ÄKTA flux s 5.4.4 Install the transfer pump Step Action Connect the communication cable to the corresponding connection on the transfer pump. Connect the power cable to the corresponding connection on the transfer pump. ÄKTA flux s Operating Instructions 29046893 AG...
  • Page 86 5 Installation 5.4 Set up the ÄKTA flux s 5.4.4 Install the transfer pump Step Action Attach the pump to ÄKTA flux s and make sure that the fittings for the screws on the chassis match the ones on the transfer pump. Fasten the screws.

  • Page 87: Install The Permeate Pump

    5 Installation 5.4 Set up the ÄKTA flux s 5.4.5 Install the permeate pump 5.4.5 Install the permeate pump Introduction This section describes how to install the optional permeate pump. Precautions WARNING Disconnect power to the product before installation of the optional pump.
  • Page 88 5 Installation 5.4 Set up the ÄKTA flux s 5.4.5 Install the permeate pump Step Action Unscrew the screws holding the casing for the permeate pump connections. Pull out the power and communication cables. ÄKTA flux s Operating Instructions 29046893 AG...
  • Page 89 5 Installation 5.4 Set up the ÄKTA flux s 5.4.5 Install the permeate pump Step Action Connect the communication cable to the corresponding connection on the permeate pump. Connect the power cable to the corresponding connection on the permeate pump. ÄKTA flux s Operating Instructions 29046893 AG...
  • Page 90 5 Installation 5.4 Set up the ÄKTA flux s 5.4.5 Install the permeate pump Step Action Attach the pump to ÄKTA flux s and make sure that the fittings for the screws on the chassis match the ones on the permeate pump. Fasten the screws.

  • Page 91: Install The Permeate Pressure Sensor

    5 Installation 5.4 Set up the ÄKTA flux s 5.4.6 Install the permeate pressure sensor 5.4.6 Install the permeate pressure sensor Introduction This section describes how to install the optional permeate pressure sensor. Precautions WARNING Disconnect power to ÄKTA flux s before installation of the permeate pressure sensor.
  • Page 92 5 Installation 5.4 Set up the ÄKTA flux s 5.4.6 Install the permeate pressure sensor Step Action Connect the power and communication cable to the connection on the pres- sure sensor. Place the pressure sensor over the fittings for the screws on the chassis so that they match.
  • Page 93 5 Installation 5.4 Set up the ÄKTA flux s 5.4.6 Install the permeate pressure sensor Step Action Fasten the screws. Calibrate the pressure sensor using the supplied factory settings. ÄKTA flux s Operating Instructions 29046893 AG...

  • Page 94: Pump Tubing

    5 Installation 5.4 Set up the ÄKTA flux s 5.4.7 Pump tubing 5.4.7 Pump tubing Install the pump tubing WARNING Disconnect power to the product before installing the pump tubing. Follow the steps below to install the pump tubing. Step Action Open the pump head by rotating the lever to the left.
  • Page 95 5 Installation 5.4 Set up the ÄKTA flux s 5.4.7 Pump tubing Step Action Route the pump tubing through the pump. Center the tubing between the retainers. NOTICE Make sure the tubing is straight where it enters and exits the pump. Twisting can cause damage to the tubing. Lightly pull on the tubing downwards to pre-stretch.
  • Page 96 5 Installation 5.4 Set up the ÄKTA flux s 5.4.7 Pump tubing Step Action Holding the tubing, rotate the lever to the right to close the pump head. Rotate the lever completely to close the pump head. NOTICE Make sure the tubing is fitted between the retainers and check that the tubing is not twisted.

  • Page 97: Installation Test

    5 Installation 5.5 Installation test Installation test Performance The following performance tests must be done after installation. • Feed pump motor control, see Section 6.4.5 Motor control, on page 126. • Tank level calibration, refer to Calibrate the tank level, on page 127 •...

  • Page 98: Run Preparations

    6 Run preparations Run preparations About this chapter This chapter provides the information required to prepare ÄKTA flux s for operation. Before ÄKTA flux s is taken into operation, make sure that all procedures in the following chapter and section have been performed: •...

  • Page 99: Filter Installation

    6 Run preparations 6.1 Filter installation Filter installation Precautions WARNING Make sure that the filter is installed according to instructions for use of the filter. WARNING Before connecting the filter to ÄKTA flux s, read the instructions for use of the filter. To avoid exposing the filter to excessive pressure, make sure that the pressure limit is set to the specified maximum pressure of the filter.

  • Page 100: Filter Holders And Tubing

    6 Run preparations 6.1 Filter installation 6.1.1 Filter holders and tubing 6.1.1 Filter holders and tubing Filter holders on ÄKTA flux s The image below shows where to attach different filter types on ÄKTA flux s. Part Function Holder for HF cartridges or Kvick Start cassette Holder for Kvick Lab Packet (optional) ÄKTA flux s Operating Instructions 29046893 AG...
  • Page 101 6 Run preparations 6.1 Filter installation 6.1.1 Filter holders and tubing Using the P1L tubing If the permeate control valve is used on the permeate side, the P1L tubing is connected to the permeate filter outlet. The descriptions on the following pages refer to installations with the optional permeate pressure sensor and the permeate pump installed.

  • Page 102: Install Hollow Fiber Cartridges

    6 Run preparations 6.1 Filter installation 6.1.2 Install hollow fiber cartridges 6.1.2 Install hollow fiber cartridges HF cartridges tubing The filter tubing listed below can be used with HF (hollow fiber) cartridges and ÄKTA flux s. Part Tubing MidGee straight Length Length MM32...
  • Page 103 6 Run preparations 6.1 Filter installation 6.1.2 Install hollow fiber cartridges HF hoop tubing The filter tubing listed below can be used with HF hoop cartridges and ÄKTA flux s. Part Tubing MidGee hoop Length Length MM32 MF32 From filter to feed 230 mm 230 mm pressure sensor...
  • Page 104 6 Run preparations 6.1 Filter installation 6.1.2 Install hollow fiber cartridges HF cartridges draining Hollow fiber cartridges are provided with two connections to the permeate compart- ment, one in each end. When mounted vertically, which is the case for ÄKTA flux s, the bottom connection serves as drain for the permeate.
  • Page 105 6 Run preparations 6.1 Filter installation 6.1.2 Install hollow fiber cartridges Step Action Connect the filter permeate outlet to the tubing going into the permeate control valve or the permeate pressure sensor inlet on the system. Attach a stop plug to the lower permeate port. Tip: Keep connected tubing short to minimize holdup volume.

  • Page 106: Install Kvick Lab Packet And Holder

    6 Run preparations 6.2 Install Kvick Lab packet and holder Install Kvick Lab packet and holder Kvick Lab packet holder tubing The filter tubing listed below can be used with Kvick Lab packet and holder and ÄKTA flux s. Part Description Length MM32...
  • Page 107 6 Run preparations 6.2 Install Kvick Lab packet and holder Filter installation CAUTION The Kvick Lab packet weighs 2.3 kg. To prevent injury, be sure to handle the holder with a secure grip. Follow the instructions below to install a Kvick Lab packet holder with installed filters onto ÄKTA flux s.

  • Page 108: Testing Filters

    It is recommended to perform a filter integrity test on each filter before usage. Perform tests according to the filter manufacturer’s instructions. This is usually some form of air diffusion test using a water wetted filter. Refer to Cytiva integrity test guides 18117269 or 18117173, or your filter manufacturer's instructions.

  • Page 109: Calibration

    6 Run preparations 6.4 Calibration Calibration Introduction This section describes the procedures used to perform calibration of the pumps and other parts in ÄKTA flux s. When to use these procedures It is recommended to perform calibrations before each run. Allowed user performed calibrations: •...

  • Page 110: Calibration Of The Feed Pump

    6 Run preparations 6.4 Calibration 6.4.1 Calibration of the feed pump 6.4.1 Calibration of the feed pump Follow the instructions below to calibrate the system flow for the feed pump. Step Action Block the retentate line to the tank by using the pinch clamp of the R2L tubing (1), and block the transfer line to the tank by adding a stopper (2) in the tank to transfer port.
  • Page 111 6 Run preparations 6.4 Calibration 6.4.1 Calibration of the feed pump Step Action Adjust the back pressure, if desired, by moving the retentate pressure control valve. Note the Pf feed pressure sensor value on the indicator panel. Remove the tubing from beaker A and put it in beaker B. Collect water between time point 0 and time point T, where T is at least one minute.
  • Page 112 6 Run preparations 6.4 Calibration 6.4.1 Calibration of the feed pump Step Action Perform the corresponding procedure (step 5 to 8) at a high rpm value for the feed pump. Tap Settings on the main screen. Tap Calibration on the screen that appears. Tap Feed pump on the Calibration Settings screen.
  • Page 113 6 Run preparations 6.4 Calibration 6.4.1 Calibration of the feed pump Step Action Enter the high rpm value of the feed pump and the corresponding calculated flow value in the gray field indicated with . Enter the saved Pf feed pressure sensor value measured in the gray field marked with Pressure.
  • Page 114 6 Run preparations 6.4 Calibration 6.4.1 Calibration of the feed pump Step Action Tap Enter. Result: The software calculates the correlation between used rpm and achieved flow and the feed pump is calibrated. To see the flow in mL/min on the main screen after calibration, set the flow rate for the feed pump to mL/min, see Set units, on page 154.

  • Page 115: Calibration Of The Transfer Pump

    6 Run preparations 6.4 Calibration 6.4.2 Calibration of the transfer pump 6.4.2 Calibration of the transfer pump Normally the transfer pump is calibrated at a back pressure of 0 Bar. If the transfer pump is to be calibrated at another back pressure, an external pressure sensor must be used together with a pinch to apply the back pressure.
  • Page 116 6 Run preparations 6.4 Calibration 6.4.2 Calibration of the transfer pump Step Action Move the tubing connected to the transfer pump outlet, from beaker A to beaker B (empty beaker), and start to collect flow at the transfer pump outlet. Collect fluid between time point 0 and time point T.
  • Page 117 6 Run preparations 6.4 Calibration 6.4.2 Calibration of the transfer pump Step Action Perform the corresponding procedure (step 2 to 5) at a high RPM value of the transfer pump. Tap Settings on the main screen. Tap Calibration on the screen that appears. Tap Transfer pump on the Calibration Settings screen.
  • Page 118 6 Run preparations 6.4 Calibration 6.4.2 Calibration of the transfer pump Step Action Enter the high RPM value of the transfer pump and the corresponding calcu- lated flow value in the gray field indicated with The transfer pump is calibrated at 0 bar back pressure. Tap Enter.
  • Page 119 6 Run preparations 6.4 Calibration 6.4.2 Calibration of the transfer pump Step Action To see the flow in mL/min on the main screen after calibration, set the flow rate for the transfer pump to mL/min, see Set units, on page 154.

  • Page 120: Calibration Of The Permeate Pump

    6 Run preparations 6.4 Calibration 6.4.3 Calibration of the permeate pump 6.4.3 Calibration of the permeate pump Follow the instructions below to calibrate the system flow for the permeate pump. The permeate pump is optional. Step Action Connect tubing to the permeate pressure sensor inlet (1) and the permeate pump outlet (2).
  • Page 121 6 Run preparations 6.4 Calibration 6.4.3 Calibration of the permeate pump Step Action Move the tubing connected to the permeate pump outlet, from beaker A to beaker B (empty beaker), and start to collect flow at the permeate pump outlet in beaker B. ÄKTA flux s Operating Instructions 29046893 AG...
  • Page 122 6 Run preparations 6.4 Calibration 6.4.3 Calibration of the permeate pump Step Action Note the Pp permeate pressure sensor value shown on the indicator panel. Collect fluid between time point 0 and time point T. Measure the mass of the fluid in beaker B with an external balance and calculate the flow rate (weight/ time).
  • Page 123 6 Run preparations 6.4 Calibration 6.4.3 Calibration of the permeate pump Step Action Enter the low RPM value of the permeate pump and the corresponding calculated flow value in the gray field indicated with . Assuming that the density is 1, the measured value in g/min will correspond to mL/min. Enter the high RPM value of the permeate pump and the corresponding calculated flow value in the gray field indicated with .
  • Page 124 6 Run preparations 6.4 Calibration 6.4.3 Calibration of the permeate pump Step Action Enter the saved Pp permeate pressure sensor value in the gray field marked with Pressure. Tap Enter. Result: The software calculates the correlation between used RPM and achieved flow and the permeate pump is calibrated.

  • Page 125: Calibration Of The Pressure Sensors

    The permeate pressure factory calibration settings for the installed unit are applied to system. Calibrate pressure sensors Contact Cytiva for a full-range calibration for the following sensors: • Feed pressure sensor Pf • Permeate pressure sensor Pp • Retentate pressure sensor Pr...

  • Page 126: Motor Control

    6 Run preparations 6.4 Calibration 6.4.5 Motor control 6.4.5 Motor control A motor control calibration is performed in order to recognize which motors that are connected to the system and shall always be performed when the optional transfer or permeate pump is installed. Follow the instructions below to perform the motor control.

  • Page 127: Tank Level

    6 Run preparations 6.4 Calibration 6.4.6 Tank level 6.4.6 Tank level Tank balance It is recommended to calibrate the tank balance prior to each run to obtain a reliable output. The tank balance must also be calibrated every time the system has been moved, or if there has been any interference with the balance.
  • Page 128 6 Run preparations 6.4 Calibration 6.4.6 Tank level Step Action Tap the gray field indicated with in Step #1: panel. Tap Enter in Step #1: panel for zero point calibration. Fill in the tank with a known mass of water measured from a measuring jar. in Step #2: panel.

  • Page 129: Operation

    7 Operation Operation About this chapter This chapter provides the information required to operate ÄKTA flux s in a safe way. In this chapter Section See page Start the system Perform a run Procedures after the run Precautions WARNING Before performing any of the procedures described in this chapter, you must read and understand all contents of the corresponding sections in Chapter 2 Safety instructions, on page...

  • Page 130: Start The System

    • Maintenance and pump tubing replacement of the ÄKTA flux s must be scheduled on regular basis with a Cytiva representative and performed by properly trained personnel only. • Always use appropriate Personal Protective Equipment (PPE) when operating and interacting with ÄKTA flux s.
  • Page 131 7 Operation 7.1 Start the system Final checks before start NOTICE Make sure that the actions listed below are completed before ÄKTA flux s is started. • Check that there is process liquid in the tank. • Check that all inlets and outlets to the system are connected, closed or in appro- priate state depending on the designed process.
  • Page 132 7 Operation 7.1 Start the system System start up Follow the steps below to start the system. Step Action Switch on the power by turning on the power switch to the I position (1). A green lamp (2) lights up. The system starts automatically and the main screen appears (3).

  • Page 133: Perform A Run

    7 Operation 7.2 Perform a run Perform a run Precautions WARNING Never block the outlet tubing and/or the check valves outlet with, for example, stop plugs, since this will create overpressure or hard- ware failure and can result in injury. CAUTION Risk of pinching by moving parts inside the tank or inside the pumps.
  • Page 134 7 Operation 7.2 Perform a run NOTICE Remove any dent in the tubing before the start of the filtering process. In this section Section See page 7.2.1 Basic filtration 7.2.2 Automated features 7.2.3 Data logging 7.2.4 Shutdown ÄKTA flux s ÄKTA flux s Operating Instructions 29046893 AG...

  • Page 135: Basic Filtration

    7 Operation 7.2 Perform a run 7.2.1 Basic filtration 7.2.1 Basic filtration Follow the steps below to perform a basic filtration. Step Action Fill the tank. Tap Mixer (1) on the control panel. Use the displayed keypad to set the desired mixer speed (rpm) and tap OK. For an instruction how to use set-points, see Adjust set-points, on page 161.

  • Page 136: Automated Features

    7 Operation 7.2 Perform a run 7.2.2 Automated features 7.2.2 Automated features Constant retentate volume The transfer pump and the level function can be used to keep a constant retentate volume (CRV) in the tank. To do this, follow the steps below. Step Action Tap Lock button (1) on Level on the control panel.
  • Page 137 7 Operation 7.2 Perform a run 7.2.2 Automated features Step Action Result: Level will control start and stop the transfer pump to keep the level of the tank constant. To stop the function, tap Lock button and use the displayed keypad to tap Deactivate.
  • Page 138 7 Operation 7.2 Perform a run 7.2.2 Automated features ΔP auto The delta pressure, measured by the feed and retentate pressure sensors, can be used to control the feed flow rate. To do this, follow the steps below. Step Action Perform a basic filtration, refer to step 1 to 5 in Section 7.2.1 Basic filtration, on page...
  • Page 139 7 Operation 7.2 Perform a run 7.2.2 Automated features Constant permeate flow The following steps require that the optional permeate pump is installed. Make sure that the feed pump is running at stable conditions before starting the permeate pump. To do this, follow the steps below. Step Action Tap Permeate (1) on the control panel.

  • Page 140: Data Logging

    7 Operation 7.2 Perform a run 7.2.3 Data logging 7.2.3 Data logging USB flash drive Data in a run can be captured on a USB flash drive. The file format is CSV (Comma Sepa- ® ® rated Value). The format CSV can be read by Microsoft Excel , for example.
  • Page 141 7 Operation 7.2 Perform a run 7.2.3 Data logging Step Action Tap Eject USB. Note: The data can be lost if this step is ignored. Manually disconnect the USB flash drive from ÄKTA flux s. Save the result file To save the result file, follow the steps below. Step Action Insert the USB flash drive in the computer.

  • Page 142: Shutdown Äkta Flux S

    7 Operation 7.2 Perform a run 7.2.4 Shutdown ÄKTA flux s 7.2.4 Shutdown ÄKTA flux s To shut down the system, follow the steps below. Step Action Tap Shutdown on the main screen. Tap Yes to power off the instrument. Note: If a restart of the system is required, tap Restart and do not proceed to step Turn off the instrument by pushing the power switch to the 0 position when...

  • Page 143: Procedures After The Run

    7 Operation 7.3 Procedures after the run Procedures after the run Introduction The procedures after the run must be performed in the following order: 1. Empty the system. 2. Clean the system and filters after every run. 3. Prepare ÄKTA flux s for storage. Precautions CAUTION Let the ÄKTA flux s cool down after use as the components and...
  • Page 144 7 Operation 7.3 Procedures after the run Prepare for storage If needed, prepare the system for storage as described in Section 9.3 Storage, on page 174. ÄKTA flux s Operating Instructions 29046893 AG...

  • Page 145: Settings

    8 Settings Settings About this chapter This chapter describes the submenus in Settings. It also includes a description on how to change the settings of the parameters. In this chapter Section See page USB Device Management screen and software update Configure Alarms screen Configure System screen Calibration Settings screen...

  • Page 146: Usb Device Management Screen And Software Update

    8 Settings 8.1 USB Device Management screen and software update USB Device Management screen and software update USB storage device panel The USB Storage Device panel shows if a USB flash drive is connected to the system, information about the type of USB flash drive and the storage capacity are displayed on the USB Storage Device panel.
  • Page 147 8 Settings 8.1 USB Device Management screen and software update Step Action Result: A text file is created onto the USB flash drive. Tap Eject USB. Remove the USB flash drive from the USB port. Software update The steps below explain how to upgrade software or install software. Step Action Format a USB flash drive in FAT32.
  • Page 148 8 Settings 8.1 USB Device Management screen and software update Step Action Wait for software to update and for the application to restart. Navigate to Settings →USB Device →USB Device Management. Select Eject USB. To get the new default values for upgradation, reset the system with the Restore default (optional step).

  • Page 149: Configure Alarms Screen

    8 Settings 8.2 Configure Alarms screen Configure Alarms screen Precautions WARNING All alarm signals must be set within the limits specified in the system documentation. Pressure and temperature control must be activated while the system is in use to prevent the tubing system to leak or break.
  • Page 150 8 Settings 8.2 Configure Alarms screen See the table below for an overview of the possible alarm or warning settings of each parameter. Parameter Alarm High Alarm Low Warning Warning High Feed pres- sure Retentate pressure Permeate pressure Tempera- ture Permeate Flow Rate Permeate...
  • Page 151 8 Settings 8.2 Configure Alarms screen Part Function A blank indicator means that no warning or alarm is set for the parameter. A green indicator shows that at least one high or low warning or alarm has been set for the parameter. A red indicator shows that an alarm or a warning is triggered.
  • Page 152 8 Settings 8.2 Configure Alarms screen Set system alarm parameters The example below shows how the Alarm High is set for feed pressure. Step Action Tap Settings on the main screen. Tap Alarms to open Configure Alarms screen. Tap Feed Pressure on the right hand of the screen. Tap the Alarm High button and use the - or + to set the preferred values for Alarm High, or use the displayed keypad to set points.
  • Page 153 8 Settings 8.2 Configure Alarms screen Step Action The Feed Pressure indicator turns green showing that an alarm high is trig- gered. Note: To deactivate an alarm or warning tap the activated alarm and check that the indicator turns white. ÄKTA flux s Operating Instructions 29046893 AG...

  • Page 154: Configure System Screen

    8 Settings 8.3 Configure System screen Configure System screen System parameters User configuration of system parameter units is set from the Configure System screen. The following units can be configured: Parameter Units Pressure bar, psi Temperature Celsius, Fahrenheit Feed Pump RPM, mL/min, Shear Rate Permeate Flux g/min, LMH...
  • Page 155 8 Settings 8.3 Configure System screen Step Action Result: The indicator on the selected button turns green. If Shear Rate is chosen as the unit for Feed Pump, you also have to set the values for Flow Rate (mL/min), and the corresponding Shear Rate (values provided by the hollow fiber manufacturer).
  • Page 156 8 Settings 8.3 Configure System screen Step Action Style Nominal Shear Rate Shear Rate Shear Rate Shear Rate Lumen ID of 2000 of 4000 of 8000 of 16000 (mm) Flow Flow Flow Flow (mL/min) (mL/min) (mL/min) (mL/min) 12.5 0.75 H22LA 0.75 H42LA AXH or...

  • Page 157: Calibration Settings Screen

    8 Settings 8.4 Calibration Settings screen Calibration Settings screen All the pumps, the pressure sensors and the tank level can be calibrated from the Cali- bration Settings screen. For information on how to perform calibration in ÄKTA flux s, Section 6.4 Calibration, on page 109 The illustration below is just an example of a calibration screen.

  • Page 158: System Information Screen

    8 Settings 8.5 System Information screen System Information screen System information The System Information panels provide information on the different parts of the system. See the screen and table below for descriptions. Part Function Description Instrument panel Provides information about installed software and the serial number of the system.
  • Page 159 8 Settings 8.5 System Information screen Part Function Description Service button Password protected sub- menu used by service personnel. System Up Time and Shows the system up Current System Time time and the current panel system time. Set System Time Sub-menu to set the button system date and time,...
  • Page 160 8 Settings 8.5 System Information screen Step Action Tap the Date fields date, month and year to set the date, and the Time (24 hr) fields hour and minutes to set the time. Use the keypad that appears to enter new values. Digits can be deleted using the arrow sign button in the upper right corner of the keypad.

  • Page 161: Set-Points

    8 Settings 8.6 Set-points Set-points Introduction This section describes how to enter set-points for Transfer, Level, Mixer, Feed, Pf auto, ΔP auto, and Permeate. Adjust set-points Follow the steps below to adjust the set-points. Step Action On the main screen, tap the parameter you want to set, for instance Transfer.
  • Page 162 8 Settings 8.6 Set-points Step Action Alternatively, tap the gray field and set the preferred values by using the displayed keypad. Digits can be deleted using the arrow sign button in the upper right corner of the keypad. Tap OK to save the settings. Tap Cancel to close the keypad without saving.
  • Page 163 8 Settings 8.6 Set-points Step Action Enter the time interval and tap OK. A run report will be of recorded at the selected log interval. ÄKTA flux s Operating Instructions 29046893 AG...

  • Page 164: Maintenance

    9 Maintenance Maintenance About this chapter This chapter provides required information to enable users and service personnel to maintain ÄKTA flux s. In this chapter Section See page User maintenance schedule Sanitization and cleaning Storage Repair and calibration Replace mains fuse Precautions WARNING Before performing any of the procedures described in this chapter,...
  • Page 165 WARNING Before maintenance/service is performed, the system owner must first clean the system and complete a Health & Safety Declaration Form. Contact Cytiva for further information. ÄKTA flux s Operating Instructions 29046893 AG...

  • Page 166: User Maintenance Schedule

    WARNING Use only approved parts. Only spare parts and accessories that are approved or supplied by Cytiva may be used for maintaining or servicing the product. For each run This section covers maintenance actions required for each run or weekly (depending on which happens first).
  • Page 167 Complete system A preventive maintenance test procedure on all systems, sensors, pumps and valves should be performed annually by trained and certified personnel. Contact your local Cytiva representative. Replace all gaskets, O-rings, and valve or pres- sure sensor diaphragms. Pump of peri- Replace all wear and tear parts.

  • Page 168: Sanitization And Cleaning

    9 Maintenance 9.2 Sanitization and cleaning Sanitization and cleaning Sanitization Sanitization is the use of chemical agents to reduce microbiological contamination to an acceptable level. A sanitization procedure is used when there is a need to reduce microbiological levels, for example between product batches, or when there has been unwanted microbiolog- ical contamination.
  • Page 169 9 Maintenance 9.2 Sanitization and cleaning CIP tubing Replace the filter with the CIP tubing during cleaning. ÄKTA flux s is cleaned by a CIP procedure with water and NaOH. Follow the steps below to clean ÄKTA flux s. The steps are described in more detail on the following pages.
  • Page 170 9 Maintenance 9.2 Sanitization and cleaning Rinse under pressure with 0.5 M NaOH. Recirculate with 0.5 M NaOH for two hours. Neutralize with water of desired quality. Note: For improved cleaning effect, the 0.5 M NaOH solution may be heated to 50°C prior to pouring it to the tank.
  • Page 171 9 Maintenance 9.2 Sanitization and cleaning C – Rinse with water under pressure To perform a rinse with water under pressure, follow the steps below. Step Action Fill the tank with water. If the optional pumps are installed, disconnect the transfer pump tubing from the transfer pump and the permeate pump tubing from the permeate pump.
  • Page 172 9 Maintenance 9.2 Sanitization and cleaning Step Action Fill the tank with 0.5 M NaOH. Drain the system. F – Rinse with 0.5 M NaOH under pressure To perform a rinse with 0.5 M NaOH under presssure, follow the steps below Step Action Fill the tank with 0.5 M NaOH.
  • Page 173 9 Maintenance 9.2 Sanitization and cleaning H – Neutralize with water To neutralize the system with water, follow the steps below. Step Action Fill the tank with water of desired quality. If the optional pumps are installed, disconnect the transfer pump tubing from the transfer pump and the permeate pump tubing from the permeate pump.

  • Page 174: Storage

    9 Maintenance 9.3 Storage Storage Introduction This section describes the procedures for storage of ÄKTA flux s. Precautions NOTICE Fit protective caps on all electrical and optical connectors when not in use. NOTICE When the product is filled with a storage solution, the temperature must be high enough to prevent freezing, and low enough to prevent evaporation.
  • Page 175 9 Maintenance 9.3 Storage Step Action Release the pump tubing from the pump head by moving the top lever from right to left. Long term storage To prevent microbial growth, the storage solution must be replaced regularly if ÄKTA flux s is stored for long periods of time. The procedure described below is applicable for storage duration longer than one month.

  • Page 176: Repair And Calibration

    WARNING Do not attempt to perform any actions not described in this docu- ment. Always contact your Cytiva representative for advice if such a need should arise. Filter integrity test This method can be used to check that the filter is not damaged and that it has been mounted correctly.

  • Page 177: Replace Mains Fuse

    Replace mains fuse Introduction A blown fuse might indicate the existence of another problem in the instrument. If a replacement fuse blows, do not replace it. Contact your Cytiva representative. Precautions WARNING Always disconnect power to ÄKTA flux s before replacing fuses.
  • Page 178 9 Maintenance 9.5 Replace mains fuse Step Action Grip the fuse drawer and loosen it. Fold the drawer lid to locate the mains fuses. Remove the old fuse with help of a small screwdriver that is inserted in the hole under the fuse drawer. ÄKTA flux s Operating Instructions 29046893 AG...
  • Page 179 9 Maintenance 9.5 Replace mains fuse Step Action Insert the new fuses. For specification of the fuses, see Electric power, on page 184. Push the fuse drawer in its holder. ÄKTA flux s Operating Instructions 29046893 AG...

  • Page 180: Troubleshooting

    This chapter provides information for users and service personnel to identify and correct problems that might occur when operating ÄKTA flux s. If the suggested actions in this guide do not solve the problem or if the problem is not covered by this guide then contact Cytiva for advice. Precautions WARNING...
  • Page 181 Pumps Pump not working • The Stop button on main screen has been pressed. • If problem persists, contact Cytiva. Little or no flow • Check that connected inlet is actually used. • Check if liquid is supplied to the system. Check inlet containers.

  • Page 182: Reference Information

    11 Reference information 11 Reference information About this chapter This chapter provides reference information that may become useful when installing, operating, maintaining and troubleshooting ÄKTA flux s. In this chapter Section See page 11.1 Specifications 11.2 Software license 11.3 Wetted materials 11.4 Chemical resistance 11.5...

  • Page 183: Specifications

    11 Reference information 11.1 Specifications 11.1 Specifications Dimensions All dimensions are presented in cm. Weight and volume Data Value Weight 28 kg Tank volume 0.5 L System sound levels Properties Value 70 dBA Typical value under normal running 65 dBA conditions ÄKTA flux s Operating Instructions 29046893 AG...
  • Page 184 11 Reference information 11.1 Specifications Electric power Property Value Supply voltage 100-120/220-240 V AC ± 10%, 50 to 60 Hz Phases Single Max power 300 VA Ingress protection IP 21 Fuses 2x T 3.15AL 250 V Over voltage category Polution degree Operating limits Parts and properties Value...
  • Page 185 11 Reference information 11.1 Specifications Parts and properties Value Max 50°C Fluid line (tubing and wetted Components and -0.4 bar g to 4.0 bar components excl. tank) piping Tank -0.1 bar to 0.05 bar Pump tubing PFD is limited to 2.5 bar. System capacity Data Pump tubing PFB...
  • Page 186 11 Reference information 11.1 Specifications MidGee MidGee Kvick Start Kvick Lab straight hoop cassette Packet MF41 MF42 MM11 MM12 MM22 MM32 MM42 MM41 Other tubing and accessories Part MM51 Tube Set 1/16 inch, 600 mm MF51 Tube Set 1/16 inch, 600 mm FF51 Tube Set 1/16 inch, 600 mm Custom male to female luer connection blue ×...

  • Page 187: Software License

    11 Reference information 11.2 Software license 11.2 Software license Software components ÄKTA flux s contains, among other things, the following software components. Embedded Linux Embedded Linux is released under GNU GPL licenses, see below. Source code can be obtained from http://www.angstrom-distribution.org. QT Libraries QT Libraries are released under GNU LGPL v2.1, see below.

  • Page 188: Wetted Materials

    11 Reference information 11.3 Wetted materials 11.3 Wetted materials Materials used The materials used in the manufacturing of ÄKTA flux s have been chosen for their biological and chemical compatibility with the solvents used during operation. List of wetted materials The table below lists the materials that come into contact with process fluids in ÄKTA flux s.
  • Page 189 11 Reference information 11.3 Wetted materials Component Materials Male Luer to 1/4-28 bottom sealing thread Female Luer to 1/4-24 bottom seal Puradisc 25 syringe filter, 0.2 μm PTFE PP (housing) Whatman filter membrane PTFE Pressure sensor Front housing Disposable gasket Silicone Female Luer to 1/4-24 bottom seal Tubing...

  • Page 190: Chemical Resistance

    11 Reference information 11.4 Chemical resistance 11.4 Chemical resistance Introduction The tables below list allowed exposure concentrations and times for various chemicals that can be used for ÄKTA flux s. WARNING Flammable liquids. ÄKTA flux s is not approved to handle flam- mable liquids.
  • Page 191 11 Reference information 11.4 Chemical resistance Allowed chemicals for wetted surfaces Chemical Concentration Max time / cycle Max acc. Usage expos. Acetic acid 3000 h Citric acid pH 2 to 2.5 1 h at temp ≤ 60°C 1000 h Ethanol 12 months Unlimited Storage...
  • Page 192 11 Reference information 11.4 Chemical resistance Allowed chemicals for outer surfaces Chemical Concentration Acetic acid Ethanol Guanidine hydrochloride Hydrogen peroxide solution Minncare cold sterilant solution (fogging) 4,5% per-acetic acid and 22% hydrogen peroxide Minncare cold sterilant solution (Wiping) 3% Minncare solution Phosphoric acid 2-propanol Sodium chloride...

  • Page 193: Recycling Information

    11 Reference information 11.5 Recycling information 11.5 Recycling information Introduction This section describes the procedures for disposal and recycling of the ÄKTA flux s. CAUTION Always use appropriate personal protective equipment when decommissioning the equipment. Decontamination The product must be decontaminated before decommissioning. All local regulations must be followed with regard to scrapping of the equipment.

  • Page 194: Regulatory Information

    11 Reference information 11.6 Regulatory information 11.6 Regulatory information Introduction This section lists the directives and standards that are fulfilled by ÄKTA flux s. In this section Section See page 11.6.1 Contact information 11.6.2 European Union and European Economic Area 11.6.3 Eurasian Economic Union Евразийский...

  • Page 195: Contact Information

    Manufacturing information The table below summarizes the required manufacturing information. Requirement Information Name and address of manufacturer Cytiva Sweden AB Björkgatan 30 SE 751 84 Uppsala Sweden Telephone number of manufacturer + 46 771 400 600 ÄKTA flux s Operating Instructions 29046893 AG...

  • Page 196: European Union And European Economic Area

    11 Reference information 11.6 Regulatory information 11.6.2 European Union and European Economic Area 11.6.2 European Union and European Economic Area Conformity with EU Directives See the EU Declaration of Conformity for the directives and regulations that apply for the CE marking. If not included with the product, a copy of the EU Declaration of Conformity is available on request.

  • Page 197: Eurasian Economic Union

    Customs Union and (or) the Eurasian Economic Union. Requirement Information Name, address and telephone See Manufacturing information number of manufacturer Importer and/or company for Cytiva RUS LLC obtaining information about 109004, Moscow importer internal city area Tagansky municipal district Stanislavsky str., 21, building 3, premises I,...
  • Page 198 получения информации об 109004, город Москва импортере вн.тер.г. муниципальный округ Таганский улица Станиславского, дом 21, строение 3, помещение I, комната 57 Российская Федерация Телефон: +7 499 609 15 50 Адрес электронной почты: rucis@cytiva.com ÄKTA flux s Operating Instructions 29046893 AG...

  • Page 199: Regulations For North America

    Note: The user is cautioned that any changes or modifications not expressly approved by Cytiva could void the user’s authority to operate the equip- ment. This equipment has been tested and found to comply with the limits for a Class A digital device, pursuant to part 15 of the FCC Rules.

  • Page 200: Regulatory Statements

    11 Reference information 11.6 Regulatory information 11.6.5 Regulatory statements 11.6.5 Regulatory statements NOTICE This equipment is not intended for use in residential environments and may not provide adequate protection to radio reception in such environments. South Korea Regulatory information to comply with the Korean technical regulations. NOTICE Class A equipment (equipment for business use).

  • Page 201: Declaration Of Hazardous Substances (Dohs)

    11 Reference information 11.6 Regulatory information 11.6.6 Declaration of Hazardous Substances (DoHS) 11.6.6 Declaration of Hazardous Substances (DoHS) This section describes the information that applies to the product in China. 根据 SJ/T11364-2014《电子电气产品有害物质限制使用标识要求》特提供如下 有关污染控制方面的信息。 The following product pollution control information is provided according to SJ/ T11364-2014 Marking for Restriction of Hazardous Substances caused by electrical and electronic products.
  • Page 202 11 Reference information 11.6 Regulatory information 11.6.6 Declaration of Hazardous Substances (DoHS) 有害物质的名称及含量 Name and Concentration of Hazardous Substances 产品中有害物质的名称及含量 Table of Hazardous Substances’ Name and Concentration 部件名称 有害物质 Compo- Hazardous substance nent name 铅 汞 镉 六价铬 多溴联苯 多溴二苯醚 (Pb) (Hg) (Cd)

  • Page 203: Other Regulations And Standards

    11 Reference information 11.6 Regulatory information 11.6.7 Other regulations and standards 11.6.7 Other regulations and standards Introduction This section describes the standards that apply to the ÄKTA flux s system. Regulatory compliance of connected equipment Any equipment connected to ÄKTA flux s must meet the safety requirements of EN/IEC 61010-1, or relevant harmonized standards.

  • Page 204: Ordering Information

    11 Reference information 11.7 Ordering information 11.7 Ordering information Contact details For ordering information, visit cytiva.com/aktaflux. ÄKTA flux s spare parts and accessories For correct up to date information on spare parts and accessories, visit cytiva.com/ aktaflux. ÄKTA flux s Operating Instructions 29046893 AG...

  • Page 205: Health And Safety Declaration Form

    Service Ticket #: To make the mutual protection and safety of Cytiva service personnel and our customers, all equipment and work areas must be clean and free of any hazardous contaminants before a Service Engineer starts a repair. To avoid delays in the servicing of your equipment, complete this checklist and present it to the Service Engineer upon arrival.
  • Page 206 To make sure the mutual protection and safety of Cytiva personnel, our customers, transportation personnel and our environment, all equipment must be clean and free of any hazardous contaminants before shipping to Cytiva. To avoid delays in the processing of your equipment, complete this checklist and include it with your return.

  • Page 207: Index

    Index Index Constant retentate volume, 60, Abbreviations, 11 Cross flow filtration, 6 Accessories, 204 CRV, 136 Air filter, 30, 45, 53 CSV, 140, 146 Air inlet to cabinet, 31 Alarms, 32, 150, 152 indicators, 150 DeltaP, 35, 39, 56 set alarm parameters, 152 Diafiltration, 50 Drain valve, 30, 53, 55 Basic filtration, 135...
  • Page 208 Index Flow path, 56 Motor control , 126 Flow range, 185 calibrate, 126 feed line, 185 permeate line, 185 transfer line, 185 Notes and tips, 6, 7 Flowchart, 52 Fuse, 181 Fuses, 31, 69, 177 replace, 177 On site service, 205 Operation, 6 prerequisites, 6 Operator touchscreen, 32...
  • Page 209 Index retentate, 30, 52 requirements, 174 Pressure sensors, 91 short term, 174 setup, 91 System alarm, 32 Product return or servicing, 206 System label, 24 Pump tubing, 73 System time, 159 Pumps, 40, 42, 47, 76, 84, 181, 184 set, 159 feed pump, 42, 184 System warning, 32 not working, 181...
  • Page 210 Index Typographical conventions, 7 Unpacking, 64 USB, 31, 146 protection, 31 Storage device panel, 146 System health report, 146 USB flash drive, 46, 70, 140 Warnings, 32 Wetted materials, 188 ÄKTA flux s Operating Instructions 29046893 AG...
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  • Page 212 © 2020 - 2021 Cytiva All goods and services are sold subject to the terms and conditions of sale of the supplying company operating within the Cytiva business. A copy of those terms and conditions is available on request. Contact your local Cytiva representative for the most current information.

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