cytiva NanoAssemblr Ignite Operating Instructions Manual page 65

Start waste example calculations
The following examples explain how to calculate the value of the Start waste param-
eter using the Ignite start waste table.
Example 1: Two same sized syringes with dilution
For a formulation with two 3 mL syringes and a 5 mL dilution syringe, the Start waste
value is calculated as follows:
Two same size syringes, 3 mL:
Dilution syringe, 5 mL:
Recommended start waste:
Syringe size (mL)
Example 2: Two different sized syringes without dilution
For a formulation with a 3 mL and a 5 mL syringe, the Start waste value is calculated as
follows:
Smaller syringe, 3 mL:
Larger syringe, 5 mL:
Recommended start waste:
Syringe size
NanoAssemblr Ignite and Ignite+ Operating Instructions NIN1134 AA
Values used to calculate Start waste for same sized
Same size syringes
1
3
5
10
20
30
Values used to calculate Start waste for different sized
(mL)
Smaller syringe
1 0.10
0.15
3
5 0.20
10 0.30
20
30
0.50
+ 0.35
= 0.85
syringes (mL)
0.3
0.50
0.70
1.10
-
-
0.15
+ 0.50
= 0.65
syringes (mL)
Larger syringe
N/A
0.35
0.50
0.80
-
-
5.1.3 Waste parameters
Dilution syringe
0.20
0.25
0.35
0.50
0.65
0.65
Dilution syringe
0.20
0.25
0.35
0.50
- 0.65
- 0.65
5 Preparation
5.1 Parameters
65