cytiva ÄKTA pilot 600 Operating Instructions Manual
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cytiva ÄKTA pilot 600 Operating Instructions Manual

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ÄKTA pilot 600
Operating Instructions
Original instructions
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Summary of Contents for cytiva ÄKTA pilot 600

  • Page 1 ÄKTA pilot 600 Operating Instructions Original instructions cytiva.com...

  • Page 2: Table Of Contents

    Table of Contents Table of Contents Introduction ......................4 About this manual ............................5 Important user information ........................6 Associated documentation ........................8 Safety instructions ..................... 11 Safety precautions ............................12 Labels ................................. 21 Emergency procedures ..........................22 System description .................... 24 Overview of the ÄKTA pilot 600 system ....................
  • Page 3 Table of Contents Prepare the flow path ..........................122 Prepare the pH monitor (optional) ......................124 Prime the flow path ............................130 Fill the air trap and the filter (optional) ....................134 Connect the columns ........................... 139 Perform a run ....................... 143 Before you start ..............................

  • Page 4: Introduction

    1 Introduction Introduction About this chapter This chapter contains important user information, descriptions of safety notices, intended use of the ÄKTA™ pilot 600 system, and lists of associated documentation. In this chapter Section See page About this manual Important user information Associated documentation ÄKTA pilot 600 Operating Instructions 29261688 AC...

  • Page 5: About This Manual

    1 Introduction 1.1 About this manual About this manual Purpose of this manual The Operating Instruction manual provides information needed to install, operate and maintain the product in a safe way. Scope of this manual The Operating Instructions cover the ÄKTA pilot 600 instrument, optional modules and accessories.

  • Page 6: Important User Information

    1 Introduction 1.2 Important user information Important user information Read this before operating the product All users must read the entire Operating Instructions before installing, oper- ating or maintaining the product. Always keep the Operating Instructions at hand when operating the product. Do not install, operate, or perform maintenance on the product in any other way than described in the user documentation.
  • Page 7 1 Introduction 1.2 Important user information Definitions This user documentation contains safety notices (WARNING, CAUTION, and NOTICE) concerning the safe use of the product. See definitions below. WARNING WARNING indicates a hazardous situation which, if not avoided, could result in death or serious injury. It is important not to proceed until all stated conditions are met and clearly understood.

  • Page 8: Associated Documentation

    Introduction This section describes the user documentation that is delivered with the product, and how to find related literature that can be downloaded or ordered from Cytiva. User documentation for ÄKTA pilot The user documentation listed in the table below. Translations of the Operating Instructions are provided on the User Documentation CD, together with the User Manual, Product Documentation, and Unpacking Instructions.
  • Page 9 Conformity other regions. Spare Part List List of spare parts available from Cytiva. UNICORN documentation The UNICORN documentation is listed in the following table. The documents are avail- able in the UNICORN Online Help and Documentation section of the online help...
  • Page 10 User documentation and other literature related to ÄKTA pilot 600 system may be downloaded from the web. Follow the steps below to access the documentation. Step Action Go to cytiva.com/aktapilot. Navigate to RELATED DOCUMENTS. Select the type of document and download the chosen literature. ÄKTA pilot 600 Operating Instructions 29261688 AC...

  • Page 11: Safety Instructions

    2 Safety instructions Safety instructions About this chapter This chapter describes safety precautions, labels and symbols that are attached to the equipment. In addition, the chapter describes emergency and recovery procedures. Important WARNING Before installing, operating or maintaining the product, all users must read and understand the entire contents of this chapter to become aware of the hazards involved.

  • Page 12: Safety Precautions

    Do not operate the product in any other way than described in the ÄKTA pilot 600 user documentation. WARNING Only properly trained personnel may operate and maintain the product. WARNING Accessories. Use only accessories supplied or recommended by Cytiva. ÄKTA pilot 600 Operating Instructions 29261688 AC...
  • Page 13 2 Safety instructions 2.1 Safety precautions WARNING Do not use the product if it is not working properly, or if it has suffered any damage, for example: • damage to the power cord or its plug • damage caused by dropping the equipment •...
  • Page 14 2 Safety instructions 2.1 Safety precautions Flammable liquids and explosive environment WARNING Flammable liquids. This product is not approved for handling flammable liquids. WARNING Explosive environment. The product is not approved for work in a potentially explosive atmosphere. The product does not fulfill the requirements of the ATEX Directive.
  • Page 15 If the product is installed on a trolley, keep the trolley wheels locked at all times except when moving the product. Power supply WARNING Power cord. Only use power cords with approved plugs delivered or approved by Cytiva. ÄKTA pilot 600 Operating Instructions 29261688 AC...
  • Page 16 2 Safety instructions 2.1 Safety precautions WARNING Protective ground. The product must always be connected to a grounded power outlet. WARNING For continued protection from fire hazard, replace only with same type and rating of fuse. Operation WARNING Before use, check that the column is not damaged or otherwise defective.
  • Page 17 2 Safety instructions 2.1 Safety precautions WARNING Overpressure. Never block the outlet tubing with, for instance, stop plugs, since this will create overpressure and might result in injury. CAUTION The flow path may remain pressurized while a run is paused. Do not loosen any connections.
  • Page 18 WARNING Use only approved parts. Only spare parts and accessories that are approved or supplied by Cytiva may be used for maintaining or servicing the product. WARNING For continued protection against injury risks due to fluid jets, burst tubes or potentially explosive atmosphere, the user must test the tubing system for leakage at maximum operating pressure.
  • Page 19 2 Safety instructions 2.1 Safety precautions WARNING Decontaminate before maintenance. To avoid exposing personnel to potentially hazardous substances, clean and sanitize the ÄKTA pilot 600 system before maintenance or service. WARNING Corrosive substance. NaOH is corrosive and therefore dangerous to health. When using hazardous chemicals, avoid spillage and wear protective glasses and other suitable Personal Protective Equipment (PPE).
  • Page 20 2 Safety instructions 2.1 Safety precautions CAUTION Always use appropriate personal protective equipment when decommissioning the equipment. ÄKTA pilot 600 Operating Instructions 29261688 AC...

  • Page 21: Labels

    2 Safety instructions 2.2 Labels Labels Introduction This section describes the system label and other safety or regulatory labels that are attached to the product. System label The system label is located on the back of the equipment. The system label identifies the equipment and shows electrical data, regulatory compliance, and warning symbols.

  • Page 22: Emergency Procedures

    2 Safety instructions 2.3 Emergency procedures Emergency procedures Introduction This section describes how to shut down the ÄKTA pilot 600 system in an emergency situation, and the procedure for restarting the instrument. The section also describes the result in the event of power failure. Precautions WARNING Access to power plug.
  • Page 23 2 Safety instructions 2.3 Emergency procedures Restart after emergency shutdown or power loss When power is restored after a power failure or emergency shutdown, perform the actions below as required: • Restart the instrument if necessary. • Restart the computer and UNICORN software. •...

  • Page 24: System Description

    3 System description System description About this chapter This chapter gives an overview of the ÄKTA pilot 600 instrument and accessories, and the UNICORN system control software. In this chapter Section See page Overview of the ÄKTA pilot 600 system Description of the ÄKTA pilot 600 instrument Tubing and connectors Available modules for ÄKTA pilot 600...

  • Page 25: Overview Of The Äkta Pilot 600 System

    Note: Adding optional modules to the ÄKTA pilot 600R system will invalidate the documentation provided with the system. Contact Cytiva if you want to modify an ÄKTA pilot 600R system. A number of accessories are available, for example an extension stand for columns. For more information, see Section 3.6 Accessories, on page...

  • Page 26: Description Of The Äkta Pilot 600 Instrument

    3 System description 3.2 Description of the ÄKTA pilot 600 instrument Description of the ÄKTA pilot 600 instrument Introduction The ÄKTA pilot 600 instrument uses a modular design, with all the liquid handling modules placed on the front of the instrument. All parts of the flow path are sanitizable. This section describes the main parts of the instrument.
  • Page 27 3 System description 3.2 Description of the ÄKTA pilot 600 instrument ÄKTA pilot 600 instrument front panel The following illustration shows an example of the ÄKTA pilot 600 instrument, with all types of modules installed. The location of modules can vary, depending on customer requirements.
  • Page 28 3 System description 3.2 Description of the ÄKTA pilot 600 instrument Part Description Label Optional Outlet Valve Outlet valve 7-9 7 8 9 Cond Pre- Pre-column Conductivity monitor column Column Valve Column valve, including pre- and post-column pres- sure sensor Bottles for pump rinsing solution Inlet Valve Inlet valve A1-A3...
  • Page 29 3 System description 3.2 Description of the ÄKTA pilot 600 instrument Description of the ON/OFF button POWER ON POWER OFF • To switch on the instrument, press the ON/OFF button. The button lights up to indi- cate the POWER ON state. •...
  • Page 30 3 System description 3.2 Description of the ÄKTA pilot 600 instrument Part Function UniNet-9 connectors, for connecting additional modules to the instru- ment. Termination plugs (jumpers) must be plugged into UniNet-9 connectors that are not used. Spare termination plugs can be stored in the holder on the back of the instrument: Illustration of the control panel The instrument control panel shows the current status of the instrument and allows...
  • Page 31 3 System description 3.2 Description of the ÄKTA pilot 600 instrument Status indications on the control panel The following table describes the different states that can be shown. The state is also shown in UNICORN. Display State Description The instrument is turned off. The Power/Communication Power on Power is on, but the instrument...
  • Page 32 3 System description 3.2 Description of the ÄKTA pilot 600 instrument Display State Description Hold A run has been put on hold. Both the Power/Communica- Pause A run has been paused by the tion indicator and Pause user. button show a steady light. CAUTION The flow path may remain pressurized while a run is...

  • Page 33: Tubing And Connectors

    4.8 mm (3/16 in) NOTICE Only use tubing intended for use with ÄKTA pilot 600 and provided by Cytiva. Using other types of tubing might cause leakage or damage to the instrument. SNAP connectors SNAP connectors are used to form a tight connection to the ports of the flow path components.
  • Page 34 3 System description 3.3 Tubing and connectors Part Function Tubing Connector Sleeve Nipple Tubing cutter A tubing cutter is included with the instrument. The illustration below shows the tubing cutter in use. ÄKTA pilot 600 Operating Instructions 29261688 AC...
  • Page 35 3 System description 3.3 Tubing and connectors Tube bender The illustration below shows the tube bender that is included with the instrument. ÄKTA pilot 600 Operating Instructions 29261688 AC...

  • Page 36: Available Modules For Äkta Pilot 600

    3 System description 3.4 Available modules for ÄKTA pilot 600 Available modules for ÄKTA pilot 600 Introduction The ÄKTA pilot 600 Standard instrument is always delivered with the standard modules installed. The Standard instrument may be expanded by adding optional modules to the flow path.
  • Page 37 3 System description 3.4 Available modules for ÄKTA pilot 600 Module Description Optional Mixer Mixes the buffers delivered from the system pumps, in a 5 mL mixer chamber, to a homogeneous buffer composition. Mixer valve A valve that directs the flow through the mixer, or bypasses the mixer, for example during sample appli- cation.
  • Page 38 3 System description 3.4 Available modules for ÄKTA pilot 600 Module Description Optional UV monitor The UV monitor is used to measure the UV/Vis absorb- ance at up to three wavelengths in the range 190-700 pH monitor The pH monitor is used to continuously measure the pH of solutions used in the run.
  • Page 39 3 System description 3.4 Available modules for ÄKTA pilot 600 Part Description UniNet-9 cable: connect to a free UniNet-9 connector on the rear panel of the instrument Port for SNAP connector Port for SNAP connector The external air sensors are numbered 1 to 4 from the factory. A label showing the number is attached to the cable.

  • Page 40: In-Line Filters

    Two alternative solutions are available for in-line filters for ÄKTA pilot 600 system: • Filter cassettes mounted in a stainless steel housing • Disposable ULTA™ Capsule CG filters from Cytiva, mounted in a filter holder Both the stainless steel housing and the filter holder are optional accessories.
  • Page 41 (optional) The ÄKTA pilot 600 ULTA CG filter mounting kit is designed for ULTA Capsule CG filters with 2" length. ULTA filters can be ordered separately from Cytiva. ULTA filters operate at a maximum pressure of 5 bar g. WARNING...

  • Page 42: Accessories

    Accessories Introduction Basic accessories are delivered with the ÄKTA pilot 600 instrument. Optional accesso- ries are available from Cytiva. This section describes the available accessories. Accessories included with the ÄKTA pilot 600 instrument The following accessories are delivered with the instrument: •...
  • Page 43 3 System description 3.6 Accessories Extension box for optional modules Optional modules can be installed in an Extension box that can be placed on the bench beside the instrument, or attached to the Extension stand. The following illustration shows a module installed in an Extension box. Part Description Retaining screw for the module...
  • Page 44 3 System description 3.6 Accessories Extension stand for columns and accessories The Extension stand can be used to mount columns, the Extension box, and the I/O- box. The stand includes holders to organize the tubing. The following illustration shows the Extension stand with a column attached. ÄKTA pilot 600 Operating Instructions 29261688 AC...
  • Page 45 3 System description 3.6 Accessories I/O-box The external I/O-box can be used to connect other equipment in order to measure parameters such as refractive index, light scattering and fluorescence. The I/O-box can communicate with external equipment by digital or analog signals. The I/O-box can be attached to the Extension stand or placed on the bench, using the supplied feet.

  • Page 46: Description Of The Flow Path

    The following illustration shows a schematic diagram of the flow path. Optional components are marked with dashed outlines. For ÄKTA pilot 600R, all components must be installed in the instrument chassis. Contact your Cytiva representative for more information about possible configura- tions. Cond...
  • Page 47 3 System description 3.7 Description of the flow path Part Function Pre-column Conductivity monitor with temperature sensor (optional) Column valve including pre-column and post-column pressure sensors Conductivity monitor with temperature sensor UV monitor pH module (optional) Outlet valve with 3 ports. Can be expanded to 15 ports with optional outlet valves.

  • Page 48: Unicorn System Control Software

    3 System description 3.8 UNICORN system control software UNICORN system control software About this section This section gives a brief overview of the UNICORN software and the System Control module that is used to control chromatography runs. For full documentation of the software, see the UNICORN user documentation. In this section Section See page...

  • Page 49: Unicorn Software Overview

    Advanced features requires Evaluation Classic, avail- able from Cytiva. When working with the modules Administration, Method Editor, System Control and Evaluation it is possible to access descriptions of the active window or software instruction by pressing the F1 key.

  • Page 50: The System Control Module

    3 System description 3.8 UNICORN system control software 3.8.2 The System Control module 3.8.2 The System Control module Introduction The System Control module is used to start, monitor, and control a manual or method run. Illustration of the System Control user interface The illustration below shows the different panes and areas of the System Control module.
  • Page 51 3 System description 3.8 UNICORN system control software 3.8.2 The System Control module System Control toolbar buttons The following table shows the System Control toolbar buttons that are referred to in this manual. Button Function UNICORN software version Up to 7.3 7.4 and later Open Method Navigator.
  • Page 52 3 System description 3.8 UNICORN system control software 3.8.2 The System Control module Process Picture overview Manual operations can be performed either via the System Control menu or the Process Picture. Each flow path component is represented by a box that displays the state of the component.
  • Page 53 3 System description 3.8 UNICORN system control software 3.8.2 The System Control module Part Function Mixer valve (optional). The state of the Mixer valve is shown in the box: In-line or By-pass. Air Trap valve. The state of the Air Trap valve is shown in the box: In-line or By-pass.
  • Page 54 3 System description 3.8 UNICORN system control software 3.8.2 The System Control module Color Indication in Flow path state Process Picture Gray Closed flow path Green dashed Open flow path without flow Green Open flow path with flow Example of dialog in the Process Picture For applicable flow path components, you can access settings by clicking the compo- nent in the Processs Picture.

  • Page 55: Installation

    4 Installation Installation About this chapter This chapter provides required information to enable users and service personnel to unpack, install, move and transport the ÄKTA pilot 600 system. In this chapter Section See page Site preparation Installing the ÄKTA pilot 600 system Connecting the system units Installing UNICORN software Connecting the ÄKTA pilot 600 instrument to UNICORN...

  • Page 56: Site Preparation

    4 Installation 4.1 Site preparation Site preparation Introduction This section describes the site planning and preparation that should be performed before the ÄKTA pilot 600 system is installed. In this section Section See page 4.1.1 Delivery, storage and unpacking 4.1.2 Room requirements 4.1.3 Site environment...

  • Page 57: Delivery, Storage And Unpacking

    When you receive the delivery • Record on the receiving documents if there is any apparent damage on the delivery box. Inform your Cytiva representative of such damage. • Move the delivery box to a protected location indoors. Delivery box ÄKTA pilot 600 instruments are shipped in a delivery box with the following dimensions...
  • Page 58 4 Installation 4.1 Site preparation 4.1.1 Delivery, storage and unpacking Equipment Specifications Pallet mover Suitable for a lightweight pallet 80 × 100 cm Cart for transporting the Dimensioned to accommodate the size and instrument to the lab weight of the instrument Unpacking the ÄKTA pilot 600 instrument For information on how to unpack the ÄKTA pilot 600 instrument and how to lift the...

  • Page 59: Room Requirements

    4 Installation 4.1 Site preparation 4.1.2 Room requirements 4.1.2 Room requirements Introduction This section describes the requirements for the room where the ÄKTA pilot 600 instru- ment is placed. Instrument dimensions and weight The outer dimensions of the ÄKTA pilot 600 instrument are shown in the following illustration.
  • Page 60 4 Installation 4.1 Site preparation 4.1.2 Room requirements Parameter Specification Minimum bench area for operating 60 x 70 cm ÄKTA pilot 600 (D x W) Minimum bench load capacity 100 kg Inclination of bench surface Horizontal ± 2° Excluding work area and area for computer. Additional depth may be required on a fixed bench to enable service access to the back of the instrument.
  • Page 61 4 Installation 4.1 Site preparation 4.1.2 Room requirements Note: When preparing the bench area for the installation, make sure that the power cord will be easy to access and disconnect. Liquid containers Place buffer containers (B) on the bench or on a shelf under the instrument. Do not place buffer containers higher than the highest point of the instrument.

  • Page 62: Site Environment

    4 Installation 4.1 Site preparation 4.1.3 Site environment 4.1.3 Site environment Introduction This section describes the environmental requirements and conditions for installation of the ÄKTA pilot 600 system. Environmental requirements The installation site must comply with the following specifications. Parameter Requirement Allowed location Indoor use only...
  • Page 63 4 Installation 4.1 Site preparation 4.1.3 Site environment Heat output The heat output data is listed in the table below. Component Heat output ÄKTA pilot 600 instrument Typically 500 W Maximum 800 W Computer, including monitor and Typically 300 W printer Refer to manufacturer's specifications for more information.

  • Page 64: Power Requirements

    WARNING Power cord. Only use power cords with approved plugs delivered or approved by Cytiva. Electrical power requirements The following table specifies the power requirements for the ÄKTA pilot 600 instru- ment. For power requirements for the computer, refer to the manufacturer's specifica- tions.
  • Page 65 4 Installation 4.1 Site preparation 4.1.4 Power requirements For UPS power requirements, see the system specifications in this manual. Remember to also take into account the specifications for the computer and monitor. Refer to the manufacturers' documentation. Note: If a UPS is used, both the ÄKTA pilot 600 instrument and computer should be connected to the UPS.

  • Page 66: Computer Requirements

    ÄKTA pilot 600 systems are controlled by UNICORN software running on an external PC. The computer is not included with the ÄKTA pilot 600 instrument. A suitable computer may be ordered from Cytiva or obtained from a third party supplier.

  • Page 67: Installing The Äkta Pilot 600 System

    Four people are required to lift the system safely. All lifting and moving must be performed in accordance with local regulations. WARNING Power cord. Only use power cords with approved plugs delivered or approved by Cytiva. ÄKTA pilot 600 Operating Instructions 29261688 AC...
  • Page 68 4 Installation 4.2 Installing the ÄKTA pilot 600 system WARNING Protective ground. The product must always be connected to a grounded power outlet. WARNING Access to power plug. Do not block access to the power outlet and power plug. The power cord with plug must always be easy to disconnect.

  • Page 69: Introduction

    4 Installation 4.2 Installing the ÄKTA pilot 600 system 4.2.1 Introduction 4.2.1 Introduction The Regulatory system is delivered with optional modules pre-installed, except for the items listed below. Optional modules purchased with the Standard system must be installed by the user. Instructions for installing the modules are provided with the modules themselves.

  • Page 70: Install The Instrument And Computer

    4 Installation 4.2 Installing the ÄKTA pilot 600 system 4.2.2 Install the instrument and computer 4.2.2 Install the instrument and computer Requirements Section 4.1.5 Computer requirements, on page 66 for computer requirements. Place the instrument on the bench Place the instrument on the bench (see Space requirements, on page 59 for space requirements).
  • Page 71 Contact your IT department or equivalent if you need help. Parameter Setting IPv4 address 172.16.0.2 Subnet mask 255.255.255.0 DHCP enabled If this address conflicts with other IP addresses in the network, contact Cytiva Service for assistance. ÄKTA pilot 600 Operating Instructions 29261688 AC...

  • Page 72: Fit Inlet And Outlet Tubing

    4 Installation 4.2 Installing the ÄKTA pilot 600 system 4.2.3 Fit inlet and outlet tubing 4.2.3 Fit inlet and outlet tubing Introduction The following tubing must be fitted: • Inlet tubing (i.d. 4.8 mm) • Outlet tubing (i.d. 3.2 mm) •...
  • Page 73 4 Installation 4.2 Installing the ÄKTA pilot 600 system 4.2.3 Fit inlet and outlet tubing Step Action Hold the tubing with one hand and rotate the tubing cutter around the tubing with the other hand until the tubing is cleanly cut. Do not squeeze the grip.
  • Page 74 4 Installation 4.2 Installing the ÄKTA pilot 600 system 4.2.3 Fit inlet and outlet tubing Step Action Make sure that the tubing end is cut at a right angle and that the surface is not damaged. If needed, cut off a short length of the tubing with the tubing cutter included with the system.
  • Page 75 4 Installation 4.2 Installing the ÄKTA pilot 600 system 4.2.3 Fit inlet and outlet tubing Step Action Press the tubing end onto the nipple, and push it all the way to the module front. Press the center part of the connector against the module front until it snaps into place.
  • Page 76 4 Installation 4.2 Installing the ÄKTA pilot 600 system 4.2.3 Fit inlet and outlet tubing Step Action Pull the sleeve to release the connector, while pushing lightly at the connector with the thumb. Remove the tubing from the nipple. Fit the waste tubing ÄKTA pilot 600 has two waste outlet ports, one from the outlet valve (Waste) and one from the column valve (Waste 2).
  • Page 77 4 Installation 4.2 Installing the ÄKTA pilot 600 system 4.2.3 Fit inlet and outlet tubing Follow the instructions below to prepare the waste tubing. Step Action Cut the waste tubing (i.d. 3.2 mm) long enough to ensure proper insertion into the waste container without becoming kinked. Remove the protective dust caps from the waste outlet(s).
  • Page 78 4 Installation 4.2 Installing the ÄKTA pilot 600 system 4.2.3 Fit inlet and outlet tubing Step Action Secure the other end of the tubing in the respective container. The container should not be above the highest point of the instrument. CAUTION Secure all outlet tubing carefully so that it remains in the container even when high flow rates cause vibrations in...

  • Page 79: Install The Air Trap

    4 Installation 4.2 Installing the ÄKTA pilot 600 system 4.2.4 Install the Air Trap 4.2.4 Install the Air Trap Introduction The Air Trap is supplied in a separate box inside the main delivery crate, and must be installed before the ÄKTA pilot 600 instrument can be used. The Air Trap (A) is mounted at the top right of the instrument front panel (B).
  • Page 80 4 Installation 4.2 Installing the ÄKTA pilot 600 system 4.2.4 Install the Air Trap Step Action Align the holes on the lower back panel of the Air Trap with the metal pins on the front of the instrument. Push the Air Trap firmly on to the pins as far as it will go. ÄKTA pilot 600 Operating Instructions 29261688 AC...
  • Page 81 4 Installation 4.2 Installing the ÄKTA pilot 600 system 4.2.4 Install the Air Trap Step Action Connect the tubing fitted to the Air Trap Valve as described as follows. • Connect the Air Trap Valve outlet (marked ) to the Air Trap inlet (A, marked •...

  • Page 82: Install The Pump Rinsing System

    4 Installation 4.2 Installing the ÄKTA pilot 600 system 4.2.5 Install the pump rinsing system 4.2.5 Install the pump rinsing system Introduction The pump rinsing system circulates rinsing solution behind the pump pistons when- ever the pumps are running. This helps to lubricate the piston seals and prevent leakage at the seals.
  • Page 83 4 Installation 4.2 Installing the ÄKTA pilot 600 system 4.2.5 Install the pump rinsing system Step Action Fill each rinsing solution bottle with 250 mL rinsing solution. Note: The recommended rinsing solution is 20% ethanol in water. Other aqueous solutions can also be used. Push the rinsing solution bottles into the holder, placing the conical bottom of the bottle in the hole in the bottom part of the holder.

  • Page 84: Fit The Ph Electrode

    4 Installation 4.2 Installing the ÄKTA pilot 600 system 4.2.6 Fit the pH electrode 4.2.6 Fit the pH electrode Introduction The optional pH module is supplied with a dummy electrode fitted in the measuring cell for both ÄKTA pilot 600S and ÄKTA pilot 600R systems. The pH electrode is packaged separately for protection.
  • Page 85 4 Installation 4.2 Installing the ÄKTA pilot 600 system 4.2.6 Fit the pH electrode Part Function Storage cup Storage solution, about 2 mL (1:1 mixture of pH 4 buffer and 1 M KNO ). This solution must be prepared by the user. Procedure Follow the instructions below to fit the pH electrode in the storage position in the pH monitor.
  • Page 86 4 Installation 4.2 Installing the ÄKTA pilot 600 system 4.2.6 Fit the pH electrode Step Action Pass the electrode cable through the locking ring so that the ring fits on to the top of the electrode with the threaded opening facing down. Carefully remove the protective plastic cover from the pH electrode.
  • Page 87 4 Installation 4.2 Installing the ÄKTA pilot 600 system 4.2.6 Fit the pH electrode Step Action Connect the pH electrode cable to the connector on the front of the pH monitor. Leave the pH electrode in the storage position until it is required for monitoring a run . Move the pH electrode to the measuring position, on page 124 for instructions on setting up the pH electrode for a run.

  • Page 88: Install Optional External Air Sensors

    4 Installation 4.2 Installing the ÄKTA pilot 600 system 4.2.7 Install optional external air sensors 4.2.7 Install optional external air sensors Introduction ÄKTA pilot 600 system supports up to 4 external air sensors (numbered 1 to 4) intended for placement in the inlet tubing to detect air entering the system. Action to be taken if air enters the system may vary and is determined by the method.
  • Page 89 4 Installation 4.2 Installing the ÄKTA pilot 600 system 4.2.7 Install optional external air sensors Step Action Connect the UniNet-9 cable from the air sensor (C) to an unused UniNet-9 port on the back of the instrument. ÄKTA pilot 600 Operating Instructions 29261688 AC...

  • Page 90: Install The Optional Steel Filter Housing

    4 Installation 4.2 Installing the ÄKTA pilot 600 system 4.2.8 Install the optional steel filter housing 4.2.8 Install the optional steel filter housing Introduction This section describes how to install the steel filter housing with filter cassette. A filter cassette in the steel housing is an alternative to the ULTA filter capsule (see Section 3.5 In-line filters, on page 40).
  • Page 91 4 Installation 4.2 Installing the ÄKTA pilot 600 system 4.2.8 Install the optional steel filter housing Step Action Insert a filter cassette into the bayonet fitting in the base of the housing and turn the cassette clockwise to lock it in place (A). Note: The filter cassette is not included with the housing, but must be purchased separately (see...
  • Page 92 4 Installation 4.2 Installing the ÄKTA pilot 600 system 4.2.8 Install the optional steel filter housing Step Action Screw the two parts of the filter housing together. Tighten by hand. Fit the in- and outlet connectors to the filter housing if they are not already fitted.
  • Page 93 4 Installation 4.2 Installing the ÄKTA pilot 600 system 4.2.8 Install the optional steel filter housing Step Action Push the Filter Housing firmly on to the pins as far as it will go. Connect the filter in the flow path using tubing and SNAP connectors as follows: •...

  • Page 94: Install The Optional Ulta Filter

    4 Installation 4.2 Installing the ÄKTA pilot 600 system 4.2.9 Install the optional ULTA filter 4.2.9 Install the optional ULTA filter Introduction This section describes how to install the ULTA filter holder and fit the filter capsule in the holder. The ULTA capsule filter is an alternative to filter cassettes in a steel housing (see Section 3.5 In-line filters, on page 40).
  • Page 95 Follow the instructions below to fit the ULTA filter capsule in the holder and connect the filter in the flow path. Note: The ULTA filter capsule is not included with the filter holder, and must be purchased separately from Cytiva. Step Action Turn the retaining screw (A) counterclockwise and lift the locking bar to open the filter holder.
  • Page 96 4 Installation 4.2 Installing the ÄKTA pilot 600 system 4.2.9 Install the optional ULTA filter Step Action Fit TC50-to-SNAP connector adapters with seals to the inlet (A) and outlet port (B) on the ULTA filter capsule, using TC50 clamps. Note: Make sure that the tightening screws on the TC50 clamps are clear of both the instrument front and the splash guard when the filter holder is closed.
  • Page 97 4 Installation 4.2 Installing the ÄKTA pilot 600 system 4.2.9 Install the optional ULTA filter Step Action Close the filter holder and tighten the retaining screw fingertight. Connect the filter in the flow path using tubing lengths and SNAP connec- tors as follows: •...
  • Page 98 4 Installation 4.2 Installing the ÄKTA pilot 600 system 4.2.9 Install the optional ULTA filter Step Action Select System →Settings from the System Control module in UNICORN software to open the System Settings dialog box. Select Pressure alarms →Alarm system pressure to present the parame- ters.

  • Page 99: Install And Uninstall Optional Modules

    Note: Installation of optional modules on a Regulatory system may require re- qualification of the system by Cytiva or an authorized representative. Note: The function of a module in the ÄKTA pilot 600 system is determined by the Node ID, a number that identifies the module in the UNICORN software.
  • Page 100 4 Installation 4.2 Installing the ÄKTA pilot 600 system 4.2.10 Install and uninstall optional modules Step Action If the instrument is switched on, switch it off using the ON/OFF button. Press the button briefly, then wait until the button illumination turns off. Choose a suitable location for the module on the instrument chassis and unscrew the retaining screw on the module panel.
  • Page 101 4 Installation 4.2 Installing the ÄKTA pilot 600 system 4.2.10 Install and uninstall optional modules Step Action Place the connector in the holder so that it does not fall into the instrument. Connect the UniNet-9 connector to the module. ÄKTA pilot 600 Operating Instructions 29261688 AC...
  • Page 102 4 Installation 4.2 Installing the ÄKTA pilot 600 system 4.2.10 Install and uninstall optional modules Step Action Fit the module in position on the instrument chassis and tighten the retaining screw. Connect the module in the flow path using suitable lengths of tubing as described in the instructions accompanying the module.
  • Page 103 Fit the module in position on the instrument chassis or in the extension box and tighten the retaining screw. Connect a UniNet-9 cable (available from Cytiva) to the UniNet-9 port on the back plate of the module (A) and an unused UniNet-9 port on the back of the ÄKTA pilot 600 instrument (B).
  • Page 104 4 Installation 4.2 Installing the ÄKTA pilot 600 system 4.2.10 Install and uninstall optional modules Step Action Add the module to the system in the UNICORN Administration (see Configure the system, on page 111). Adjust the delay volume if the module is installed downstream of the UV monitor (see Section 4.7 Delay volumes, on page 115).
  • Page 105 4 Installation 4.2 Installing the ÄKTA pilot 600 system 4.2.10 Install and uninstall optional modules Step Action For modules installed on the instrument chassis, disconnect the UniNet-9 connector from the back of the module. If the module is not being replaced, connect the UniNet-9 connector to back of the module panel, and replace the module panel on the instrument.

  • Page 106: Connecting The System Units

    • Power cord with EU-plug, 2 m Discard the unused power cord. Note: Contact Cytiva for minimum specifications if you need to use a longer power cord. Connect the power cord to the mains input connector on the back of the instrument and to a grounded wall outlet 100 to 240 VAC, 50 to 60 Hz.
  • Page 107 4 Installation 4.3 Connecting the system units Step Action Secure the cable in the holder on the back of the instrument. Connect the ÄKTA pilot 600 instrument to the computer Connect a network cable between the CTRL connector (network) on the back of the instrument and the computer network port set up for connection to the instrument (see Network settings, on page...

  • Page 108: Installing Unicorn Software

    UNICORN software. The instructions apply to both the initial installation of UNICORN software and to installation of updated Instrument Configuration files. Step Action Go to cytiva.com/aktapilot. Click RELATED DOCUMENTS. Open SOFTWARE. Download the Instrument configuration software from the SOFTWARE section.

  • Page 109: Connecting The Äkta Pilot 600 Instrument To Unicorn Software

    4 Installation 4.5 Connecting the ÄKTA pilot 600 instrument to UNICORN software Connecting the ÄKTA pilot 600 instrument to UNICORN software Introduction This section describes how to start the system and connect the ÄKTA pilot 600 instru- ment to UNICORN software. Start the ÄKTA pilot 600 instrument Follow the instructions below to start the ÄKTA pilot 600 instrument.
  • Page 110 4 Installation 4.5 Connecting the ÄKTA pilot 600 instrument to UNICORN software Note: Screen illustrations are taken from UNICORN version 7.3. Appearance may differ in other UNICORN versions. Step Action Double-click the UNICORN icon on the desktop. Result: The Log On dialog box opens. Enter your username and password.
  • Page 111 4 Installation 4.5 Connecting the ÄKTA pilot 600 instrument to UNICORN software Step Action In the System Control module, click the Connect to Systems button. Result: The Connect to Systems dialog box opens. In the Connect to Systems dialog box: •...
  • Page 112 4 Installation 4.5 Connecting the ÄKTA pilot 600 instrument to UNICORN software Step Action Open System Properties in the UNICORN Administration module. Select the desired system and click Edit. ÄKTA pilot 600 Operating Instructions 29261688 AC...
  • Page 113 4 Installation 4.5 Connecting the ÄKTA pilot 600 instrument to UNICORN software Step Action Choose the appropriate Component type and check the required compo- nent(s). Click OK. A warning that the system will be disconnected will appear. Click Yes. To reconnect the system, choose Connect to Systems from the Tools menu in System Control.

  • Page 114: Performance Tests

    4 Installation 4.6 Performance tests Performance tests Recommendation To check the perfomance of a newly installed system, selected performance tests may be run. The tests are not a mandatory part of the installation process but can help to establish that the installation has been performed correctly. Performance test procedures are described in ÄKTA pilot 600 User Manual.

  • Page 115: Delay Volumes

    4 Installation 4.7 Delay volumes Delay volumes Introduction The delay volume is the volume of the flow path between the detector (normally the UV Monitor) and the outlet, and represents the delay between detection of material and appearance of the material at the outlet. Delay volumes are specified as the volume to the inlet port on each Outlet Valve.
  • Page 116 4 Installation 4.7 Delay volumes Component Length Volume pH Monitor internal volume 0.77 mL Tubing from pH Monitor to Outlet Valve 1-3 13 cm 1.04 mL Delay volume from UV Monitor to Outlet Valve 1-3 3.0 mL Enter the delay volume in UNICORN Follow the instructions below to set the delay volume in UNICORN.

  • Page 117: Moving The Äkta Pilot 600 System

    4 Installation 4.8 Moving the ÄKTA pilot 600 system Moving the ÄKTA pilot 600 system Introduction This section provides instructions for moving the ÄKTA pilot 600 system within and between sites. For transport over longer distances, pack the instrument securely in a protective box. Use the original packing material if possible.
  • Page 118 4 Installation 4.8 Moving the ÄKTA pilot 600 system Step Action Remove the following components: • Any column with associated tubing • Bottles connected to the instrument • Inlet, outlet, and waste tubing • Tubing and connections to modules installed in a free-standing Extension Box.
  • Page 119 4 Installation 4.8 Moving the ÄKTA pilot 600 system Step Action Reconnect the system units (see Section 4.3 Connecting the system units, on page 106). Place bottles and tubes as required. Prepare the waste tubing and pump rinsing system (see Section 4.2.3 Fit inlet and outlet tubing, on page 72 Section 4.2.5 Install the pump rinsing...

  • Page 120: Prepare The System For A Run

    5 Prepare the system for a run Prepare the system for a run About this chapter This chapter gives instructions on how to prepare the ÄKTA pilot 600 system for a run. Prerequisites The instrument must be properly installed in accordance with the instructions in Chapter 4 Installation, on page 55 Before the user can log on and connect to the instrument, an appropriate User account must have been created in UNICORN.

  • Page 121: Start The Instrument And Software

    5 Prepare the system for a run 5.1 Start the instrument and software Start the instrument and software Start and log on Follow the instructions in Section 4.5 Connecting the ÄKTA pilot 600 instrument to UNICORN software, on page 109 to start the instrument and software if either compo- nent is not started.

  • Page 122: Prepare The Flow Path

    5 Prepare the system for a run 5.2 Prepare the flow path Prepare the flow path Introduction The flow path contains tubing, valves, pumps, mixer, air trap and monitors. This section describes how to prepare the flow path inclusive mixer (optional) and filter (optional), but except a pH monitor (optional).
  • Page 123 5 Prepare the system for a run 5.2 Prepare the flow path Step Action Disconnect the tube ends that connects the steel housing to the flow path. Disconnect the steel housing from the metal pins and carry it to a sink. Open the top valve so that the fluid flows out into the sink (or to a container appropriate for the fluid).

  • Page 124: Prepare The Ph Monitor (Optional)

    5 Prepare the system for a run 5.3 Prepare the pH monitor (optional) Prepare the pH monitor (optional) Introduction The pH monitor is an optional module of ÄKTA pilot 600. This section describes how to move the pH electrode between storage position and run position, and how to perform a calibration.
  • Page 125 5 Prepare the system for a run 5.3 Prepare the pH monitor (optional) Step Action Unscrew the locking ring on the pH electrode (A) and lift the pH electrode out of the storage cup (B). Insert the pH electrode into the measuring cell (A). Tighten the locking ring by hand (B).
  • Page 126 5 Prepare the system for a run 5.3 Prepare the pH monitor (optional) Step Action Insert the dummy electrode into the storage cup. Tighten the locking ring by hand. Make sure that the pH electrode cable is well connected to the front of the pH monitor.
  • Page 127 5 Prepare the system for a run 5.3 Prepare the pH monitor (optional) Step Action In the System Control module, choose System →Calibrate to open the Calibration dialog. Select pH, and press Prepare for calibration. Enter the pH value of the first calibration solution at pH for buffer 1. Insert a syringe filled with deionized water to the manual injection port on the pH monitor.
  • Page 128 5 Prepare the system for a run 5.3 Prepare the pH monitor (optional) Step Action Open the port (A), inject approximately 20 mL deionized water (B), and close the port. Note: Do not let the port be open without a syringe attached, because air inside the flow cell will impair the calibration.
  • Page 129 5 Prepare the system for a run 5.3 Prepare the pH monitor (optional) Step Action Open the port, slowly inject approximately 20 mL of the second calibration solution into the flow cell, and close the port. When the displayed Current value is stable, press Calibrate. Record the values displayed for the slope and asymmetry potential.

  • Page 130: Prime The Flow Path

    5 Prepare the system for a run 5.4 Prime the flow path Prime the flow path Introduction The flow path needs to be primed before a run. Priming the flow path can be done using the Process Picture or via manual instructions in the System Control module or by creating and running a system preparation method.
  • Page 131 5 Prepare the system for a run 5.4 Prime the flow path Step Action Click Inlet B to open the Inlet B dialog box, and select an inlet port. If the flow path does not change color because the run is paused or stopped, click Continue in the System Control toolbar.
  • Page 132 5 Prepare the system for a run 5.4 Prime the flow path Step Action Approximately 15 seconds after the last B port is primed, redirect the flow to use pump A: a. Click Inlet A and select an inlet port. b.
  • Page 133 5 Prepare the system for a run 5.4 Prime the flow path Step Action Start pump A at a high flow rate: a. Click Pump A to open the System pumps dialog box. b. Set Conc % B to 0%, and click Set below. c.

  • Page 134: Fill The Air Trap And The Filter (Optional)

    5 Prepare the system for a run 5.5 Fill the air trap and the filter (optional) Fill the air trap and the filter (optional) Introduction The air trap tubing should be washed and the air trap should be filled with a suitable solution even if the air trap will not be in-line during the run.
  • Page 135 5 Prepare the system for a run 5.5 Fill the air trap and the filter (optional) Step Action Click Start fill. Result: The following dialog box opens. Open the manual valve on the air trap. Tip: Fit a tube from the top SNAP connector to waste, to avoid spillage when the air trap is completely filled.
  • Page 136 5 Prepare the system for a run 5.5 Fill the air trap and the filter (optional) Step Action Press the Continue button on the instrument control panel, keep an eye on the increasing amount of liquid in the air trap, and be prepared to press Continue again as described in the next step.
  • Page 137 5 Prepare the system for a run 5.5 Fill the air trap and the filter (optional) Step Action Close the manual valve on the air trap, and click Continue. Result: The post wash starts, and the following dialog box opens. During the post wash of the tubing, it is possible to abort the filling by clicking Abort in the POST WASH dialog box.
  • Page 138 5 Prepare the system for a run 5.5 Fill the air trap and the filter (optional) Step Action Open the air vent valve on the filter (A) and insert a syringe (B) without the plunger. The illustration shows the steel filter housing, the ULTA filter has a similar air vent valve.

  • Page 139: Connect The Columns

    • Make sure you have read the instruction for the selected column. • Make sure you have appropriate tubing and unions. There are more unions available from Cytiva than the unions enclosed at delivery. • Make sure the flow path is primed with a suitable buffer, see Section 5.4 Prime the...
  • Page 140 5 Prepare the system for a run 5.6 Connect the columns Step Action Set the delta column pressure: a. In the Delta column pressure panel, click HIGH ALARM. b. In the pop-up window, set the High limit value for the selected column. c.
  • Page 141 5 Prepare the system for a run 5.6 Connect the columns Step Action Disconnect the column top tubing. Fill the disconnected tubing: a. Click Outlet and select Waste/Out 1, to open the flow path. b. Click Column valve, select Column 1 downflow and click Set posi- tion, to redirect the flow.
  • Page 142 5 Prepare the system for a run 5.6 Connect the columns Step Action When the disconnected tubing is filled, reconnect it drop-to-drop to the top of the column. Make sure that there is no air in the connection. When the tubing at the bottom of the column is filled, click the End button in the System Control toolbar.

  • Page 143: Perform A Run

    6 Perform a run Perform a run About this chapter This chapter gives instructions on how to safely perform a run using the ÄKTA pilot 600 system. In this chapter Section See page Before you start Manual operations Sample application Start a method run Monitor the run Procedures after the run...

  • Page 144: Before You Start

    6 Perform a run 6.1 Before you start Before you start Introduction It is necessary to read and understand the information in this section and to perform the finals checks before start. Safety precautions WARNING High pressure. The flow rate may under no circumstances exceed the specified column maximum flow rate.
  • Page 145 6 Perform a run 6.1 Before you start • The system pressure alarm limit is adjusted to the component before the column with the lowest pressure limit. (Use Process Picture →System pressure →HIGH ALARM.) • The system is prepared according to the settings in the method to be run. •...

  • Page 146: Manual Operations

    6 Perform a run 6.2 Manual operations Manual operations Introduction Manual operations are performed from the System Control module in UNICORN. Note: Any manual interactions performed during a manual or method run will be logged and saved in the run result. Manual instructions dialog box All manual instructions are available from the Manual instructions dialog box.

  • Page 147: Sample Application

    6 Perform a run 6.3 Sample application Sample application Introduction This section describes how to select sample application technique and parameter values in a UNICORN method. See the UNICORN Method Editor Manual for information about how to create and edit a method.
  • Page 148 6 Perform a run 6.3 Sample application Step Action In the Sample Application phase, open the Phase Properties tab. Select Inject sample directly onto column (Inject sample directly onto column). Select Sample inlet (A inlet). Make sure Inject fixed sample volume is selected, and enter the sample volume.
  • Page 149 6 Perform a run 6.3 Sample application Inject sample from Superloop, manual load Follow the instructions below to modify the sample application phase in the method, to use a Superloop. Step Action In the Sample Application phase, open the Phase Properties tab. Select Inject sample from Superloop, manual load (Inject sample from Superloop, manual load).

  • Page 150: Start A Method Run

    6 Perform a run 6.4 Start a method run Start a method run Introduction This section describes how to start a method run in the UNICORN System Control module. Method run Follow the instructions below to open and start a method run in UNICORN. Note: For creating a method refer to UNICORN Method Manual.

  • Page 151: Monitor The Run

    6 Perform a run 6.5 Monitor the run Monitor the run Introduction The run is monitored in the UNICORN System Control module as follows: • The run progress is monitored in the Chromatogram pane. • The current flow path and current numerical data from monitors are shown in the Process Picture.

  • Page 152: Procedures After The Run

    6 Perform a run 6.6 Procedures after the run Procedures after the run Introduction After the run, the instrument and column should be cleaned to prevent bacterial growth, sample contamination in the next run, and column clogging. This section describes how to clean or sanitize the instrument, prepare the instrument for storage and how to shut down the system.
  • Page 153 6 Perform a run 6.6 Procedures after the run Step Action Change the pump rinsing solution, use 20% ethanol. See Section 7.3.1 Change the pump rinsing solution, on page 166. Clean the system by running the System CIP or the System Sanitization method.

  • Page 154: Maintenance

    WARNING Use only approved parts. Only spare parts and accessories that are approved or supplied by Cytiva may be used for maintaining or servicing the product. ÄKTA pilot 600 Operating Instructions 29261688 AC...
  • Page 155 7 Maintenance WARNING For continued protection against injury risks due to fluid jets, burst pipes or potentially explosive atmosphere, the user must test the piping system for leakage at maximum operating pressure. • Always perform a leakage test after assembly or maintenance. •...

  • Page 156: Maintenance Schedule

    7 Maintenance 7.1 Maintenance schedule Maintenance schedule Introduction Maintenance procedures to be performed by the user are outlined below. Periodic maintenance The following periodic maintenance should be performed by the user of the ÄKTA pilot 600 system. Interval Maintenance action Instructions Daily or before each Calibrate the pH monitor.
  • Page 157 7 Maintenance 7.1 Maintenance schedule Maintenance action Instructions Clean and store the pH electrode Section 7.2.4 Clean and store the pH electrode, on page 163 Clean the conductivity flow cell See ÄKTA pilot 600 User Manual Calibrate the conductivity monitor See ÄKTA pilot 600 User Manual Replace tubing and connectors...

  • Page 158: Cleaning Procedures

    7 Maintenance 7.2 Cleaning procedures Cleaning procedures About this section This section describes procedures for cleaning the ÄKTA pilot 600 instrument and components, and also for storing and regenerating the pH electrode. Note: For column cleaning and storage procedures, refer to the column instruc- tions.

  • Page 159: Cleaning Before Planned Service

    7 Maintenance 7.2 Cleaning procedures 7.2.1 Cleaning before planned service 7.2.1 Cleaning before planned service Cleaning before planned maintenance/service To ensure the protection and safety of service personnel, all equipment and work areas must be clean and free of any hazardous contaminants before a Service Engineer starts maintenance work.

  • Page 160: Clean The Instrument Surfaces

    7 Maintenance 7.2 Cleaning procedures 7.2.2 Clean the instrument surfaces 7.2.2 Clean the instrument surfaces NOTICE Cleaning. Keep the exterior of the instrument dry and clean. Wipe regularly with a soft damp tissue and, if necessary, a mild cleaning agent. Let the instrument dry completely before use. Maintenance interval Clean the the instrument externally when required.

  • Page 161: Clean The Flow Path

    Always run System CIP or System Sanitization using 20% ethanol to fill the flow system as the final step before long-term storage. Always run System Sanitization before maintenance by Cytiva service personnel. ÄKTA pilot 600 Operating Instructions 29261688 AC...
  • Page 162 7 Maintenance 7.2 Cleaning procedures 7.2.3 Clean the flow path Required materials The following items may be required: • About 85 cm tubing with SNAP connectors (i.d. 3.2 mm) to connect the manual valve on the Air Trap to the Intelligent Packing (IP) port on the Column Valve. •...

  • Page 163: Clean And Store The Ph Electrode

    7 Maintenance 7.2 Cleaning procedures 7.2.4 Clean and store the pH electrode 7.2.4 Clean and store the pH electrode NOTICE Never leave the pH electrode in the flow cell when the system is not in use. This may allow the glass membrane of the electrode to dry out.
  • Page 164 7 Maintenance 7.2 Cleaning procedures 7.2.4 Clean and store the pH electrode Step Action Heat a 1 M KNO solution to 60°C to 80°C. Place the electrode tip in the heated KNO solution. Allow the electrode to cool while immersed in the KNO solution before re- testing.

  • Page 165: Replacement Procedures

    7 Maintenance 7.3 Replacement procedures Replacement procedures About this section This section gives instructions for the replacement procedures to be performed by the user of ÄKTA pilot 600 instrument. In this section Section See page 7.3.1 Change the pump rinsing solution 7.3.2 Replace pump rinse tubing 7.3.3...

  • Page 166: Change The Pump Rinsing Solution

    7 Maintenance 7.3 Replacement procedures 7.3.1 Change the pump rinsing solution 7.3.1 Change the pump rinsing solution Pump rinsing solutions The recommended pump rinsing solution is 20% ethanol. Using this solution will help to prevent bacterial growth in the pump rinsing system. Buffer or distilled water may also be used if desired.
  • Page 167 7 Maintenance 7.3 Replacement procedures 7.3.1 Change the pump rinsing solution Step Action Pull out the two pump rinsing bottles from the holders. Note: You may need to push the lower part of the bottle holders downwards to release the bottles. Empty and rinse out the bottles.

  • Page 168: Replace Pump Rinse Tubing

    7 Maintenance 7.3 Replacement procedures 7.3.2 Replace pump rinse tubing 7.3.2 Replace pump rinse tubing Maintenance interval Replace the pump rinsing system tubing when required, for example if the tubing is clogged or damaged. Required material The following material is required: •...
  • Page 169 7 Maintenance 7.3 Replacement procedures 7.3.2 Replace pump rinse tubing Connect new tubing Step Action Disconnect the used tubing. Connect the new tubing according to the illustration above. Note: Fit the clips from the original pump rinsing tubing. Do not attempt to use SNAP connectors.

  • Page 170: Replace Flow Path Tubing And Connectors

    7 Maintenance 7.3 Replacement procedures 7.3.3 Replace flow path tubing and connectors 7.3.3 Replace flow path tubing and connectors Maintenance interval Replace tubing and connectors when required, for example when a tubing has clogged or has become kinked so that the flow is stopped or when the end of the tubing is worn and there is a risk for leakage.

  • Page 171: Replace Valve Membranes

    7 Maintenance 7.3 Replacement procedures 7.3.4 Replace valve membranes 7.3.4 Replace valve membranes Introduction This section describes when and how to replace the valve membranes in the ÄKTA pilot 600 instrument. Valves with replaceable membranes The instructions in this section apply to membranes in the valves listed below. The procedure for replacing valve membranes is the same for all valves, although the number of membranes varies between the valves.
  • Page 172 7 Maintenance 7.3 Replacement procedures 7.3.4 Replace valve membranes Required tools Tool Dimension Torque wrench, up to 3.5 Nm (not 7 mm supplied) Procedure Follow the instructions below to replace the valve membranes. Do not switch off the instrument during membrane replacement. Note: In the instructions below, the term <valve name>...
  • Page 173 7 Maintenance 7.3 Replacement procedures 7.3.4 Replace valve membranes Step Action Remove the bolts that secure the valve front to the instrument. There are 6 bolts on the Column Valve and 3 on other valves. Air Trap Valve and other valves Column Valve NOTICE Remove only the bolts indicated in the illustrations...
  • Page 174 7 Maintenance 7.3 Replacement procedures 7.3.4 Replace valve membranes Step Action Set <valve name> maintenance position to Closed in the UNICORN software and click Execute. Leave the Manual Instruction dialog open. Result: the valve plungers move a few mm out of the valve body. Remove the old valve membranes from the valve body.
  • Page 175 7 Maintenance 7.3 Replacement procedures 7.3.4 Replace valve membranes Step Action Set <valve name> maintenance position to Open All in the UNICORN software. Result: the valve plungers with the new membranes move a few mm into the valve body. Make sure that the membranes are correctly seated in the valve body. Replace the valve front on the valve body and secure it with the bolts.
  • Page 176 7 Maintenance 7.3 Replacement procedures 7.3.4 Replace valve membranes Step Action Select <valve name> confirm membrane change in the UNICORN soft- ware Manual Instructions dialog. Click Execute. Close the Manual Instructions dialog. Click the End button on the UNICORN toolbar to put the system in the Ready state.

  • Page 177: Replace The Main Fuses

    7 Maintenance 7.3 Replacement procedures 7.3.5 Replace the main fuses 7.3.5 Replace the main fuses WARNING Disconnect power. Always disconnect power from the instru- ment before replacing fuses. WARNING For continued protection from fire hazard, replace only with same type and rating of fuse. WARNING If a fuse requires repeated replacement, do not continue to use the instrument.
  • Page 178 7 Maintenance 7.3 Replacement procedures 7.3.5 Replace the main fuses Step Action Use a small flat-bladed screwdriver to prise open the fuse holder cover on the power inlet. Pull the fuse holder out of the mains connector panel by hand. Remove the fuses from the fuse holder.

  • Page 179: Troubleshooting

    This chapter does not cover troubleshooting for chromatography applications. Publi- cations describing general chromatography principles and practice are available from Cytiva. If you cannot resolve problems using the information provided here, call your Cytiva service representative. System error report When you request troubleshooting assistance from Cytiva, you should generate a System error report (system error report) and submit it to your service representa- tive.
  • Page 180 Check the power outlet and power cord. start. • Fuse needs replacing Section 7.3.5 Replace the main fuses, on page 177. If the problem persists, call your Cytiva service repre- sentative. Software and communication problems Problem Possible cause and action Unable to connect to •...
  • Page 181 8 Troubleshooting Flow path problems Problem Possible cause and action Leakage from a valve • Valve membranes need replacing front Section 7.3.4 Replace valve membranes, on page 171. Leakage from a • Tubing and SNAP connector not correctly fitted tubing connection Replace the tubing.

  • Page 182: Reference Information

    9 Reference information Reference information About this chapter This chapter provides reference information for the ÄKTA pilot 600 system. Refer to the ÄKTA pilot 600 Product Documentation for additional details and for specific information relating to your system. In this chapter Section See page System specifications...

  • Page 183: System Specifications

    9 Reference information 9.1 System specifications System specifications Technical specifications Parameter Data System configuration Benchtop system, external computer Control system UNICORN 7.3 or later version Connection between PC and instru- Ethernet ment Dimensions (width × height × depth) 575 × 865 × 510 mm Weight (excluding computer, columns, 77 to 84 kg buffer bottles)

  • Page 184: Tubing And Connectors

    9 Reference information 9.2 Tubing and connectors Tubing and connectors Introduction This section describes the tubing and connectors that are used with the ÄKTA pilot 600 system. Tubing Tubing dimensions are given in the table below. Location i.d. o.d. Material Volume/cm (approx) (approx)

  • Page 185: Chemical Resistance Guide

    9 Reference information 9.3 Chemical resistance guide Chemical resistance guide Introduction This section provides general information about biocompatibilty and detailed informa- tion about chemical resistance of the ÄKTA pilot 600 instrument. NOTICE The information in this section applies to the ÄKTA pilot 600 instru- ment.

  • Page 186: General Information

    9 Reference information 9.3 Chemical resistance guide 9.3.1 General information 9.3.1 General information Biocompatibility The ÄKTA pilot 600 instrument is designed for maximum biocompatibility, with biochemically inert flow paths constructed mainly from titanium, PEEK and highly resistant fluoropolymers and fluoroelastomers. Plastics and rubber materials are selected to avoid leakage of monomers, plasticizers or other additives.

  • Page 187: Chemical Resistance Specifications

    This section provides detailed information about chemical resistance of the ÄKTA pilot 600 instrument to some common aggressive chemicals used in liquid chromatog- raphy. For information regarding chemicals not listed in this section, contact your Cytiva representative. Note: Refer to Safety Data Sheets (SDS) for information regarding characteristics, human and environmental risks and preventive measures for chemicals used.
  • Page 188 9 Reference information 9.3 Chemical resistance guide 9.3.2 Chemical resistance specifications Chemical Concentration CAS no EC no Ammonia 7664-41-7 231-635-3 Ammonium chloride 12125-02-9 235-186-4 Ammonium sulfate 7783-20-2 231-984-1 Arginine 74-79-3 200-811-1 Benzyl alcohol 100-51-6 202-859-9 Dimethyl sulfoxide (DMSO) 67-68-5 200-664-3 Dithioerythritol (DTE) 100 mM 6892-68-8...

  • Page 189: Recycling Information

    Recycling of hazardous substances The product contains hazardous substances. Detailed information is available from your Cytiva representative. Disposal of electrical components Waste electrical and electronic equipment must not be disposed of as unsorted munic- ipal waste and must be collected separately.

  • Page 190: Regulatory Information

    9 Reference information 9.5 Regulatory information Regulatory information Introduction This section lists the regulations and standards that apply to the product. In this section Section See page 9.5.1 Contact information 9.5.2 European Union and European Economic Area 9.5.3 Eurasian Economic Union Евразийский...

  • Page 191: Contact Information

    Manufacturing information The table below summarizes the required manufacturing information. Requirement Information Name and address of manufacturer Cytiva Sweden AB Björkgatan 30 SE 751 84 Uppsala Sweden Telephone number of manufacturer + 46 771 400 600 ÄKTA pilot 600 Operating Instructions 29261688 AC...

  • Page 192: European Union And European Economic Area

    9 Reference information 9.5 Regulatory information 9.5.2 European Union and European Economic Area 9.5.2 European Union and European Economic Area Introduction This section describes regulatory information for the European Union and European Economic Area that applies to the equipment. Conformity with EU Directives See the EU Declaration of Conformity for the directives and regulations that apply for the CE marking.

  • Page 193: Eurasian Economic Union

    Presnenskaya nab., 10, fl. 12, pr. III, room 6 Telephone: + 7 495 739 6931 Fax nr: + 7 495 739 6932 E-mail: rucis@cytiva.com Информация о производителе и импортере В следующей таблице приводится сводная информация о производителе и импортере, согласно требованиям Технических регламентов Таможенного союза...
  • Page 194 Пресненская наб., д. 10, эт. 12, пом. III, ком. 6 Телефон: + 7 495 739 6931 Факс: + 7 495 739 6932 Адрес электронной почты: rucis@cytiva.com Description of symbol on the system label Описание обозначения на этикетке системы This Eurasian compliance mark indicates that the product is...

  • Page 195: Regulations For North America

    Note: The user is cautioned that any changes or modifications not expressly approved by Cytiva could void the user’s authority to operate the equip- ment. This equipment has been tested and found to comply with the limits for a Class A digital device, pursuant to part 15 of the FCC Rules.

  • Page 196: Regulatory Statements

    9 Reference information 9.5 Regulatory information 9.5.5 Regulatory statements 9.5.5 Regulatory statements Introduction This section shows regulatory statements that apply to regional requirements. EMC emission, CISPR 11: Group 1, Class A statement NOTICE This equipment is not intended for use in residential environments and may not provide adequate protection to radio reception in such environments.

  • Page 197: Declaration Of Hazardous Substances (Dohs)

    9 Reference information 9.5 Regulatory information 9.5.6 Declaration of Hazardous Substances (DoHS) 9.5.6 Declaration of Hazardous Substances (DoHS) 根据 SJ/T11364-2014《电子电气产品有害物质限制使用标识要求》特提供如下 有关污染控制方面的信息。 The following product pollution control information is provided according to SJ/ T11364-2014 Marking for Restriction of Hazardous Substances caused by electrical and electronic products.
  • Page 198 9 Reference information 9.5 Regulatory information 9.5.6 Declaration of Hazardous Substances (DoHS) 有害物质的名称及含量 Name and Concentration of Hazardous Substances 产品中有害物质的名称及含量 Table of Hazardous Substances’ Name and Concentration 部件名称 有害物质 Compo- Hazardous substance nent name 铅 汞 镉 六价铬 多溴联苯 多溴二苯醚 (Pb) (Hg) (Cd)

  • Page 199: Other Regulations And Standards

    9 Reference information 9.5 Regulatory information 9.5.7 Other regulations and standards 9.5.7 Other regulations and standards Introduction This section describes the standards that apply to the product. Biological and chemical compatibility The Wetted parts of the ÄKTA pilot 600 instrument meet the material requirements of the following standards and regulations: Requirement Description...

  • Page 200: Health And Safety Declaration Form

    Service Ticket #: To make the mutual protection and safety of Cytiva service personnel and our customers, all equipment and work areas must be clean and free of any hazardous contaminants before a Service Engineer starts a repair. To avoid delays in the servicing of your equipment, complete this checklist and present it to the Service Engineer upon arrival.
  • Page 201 To make sure the mutual protection and safety of Cytiva personnel, our customers, transportation personnel and our environment, all equipment must be clean and free of any hazardous contaminants before shipping to Cytiva. To avoid delays in the processing of your equipment, complete this checklist and include it with your return.

  • Page 202: Index

    Index Index box transportation, 57 Documentation, 9, 10 Accessories, 38, 42–45 on the web, 10 extension box, 43 Documentation package, 8, 9 extension stand, 44 regulatory version, 9 external air sensors, 38 standard version, 8 I/O-box, 45 included with the instrument, Emergency, 22 optional, 42 procedures, 22...
  • Page 203 Index Interrupt a method, 151 Prepare the system, 109, 122–124, 126, 134, 137 air trap, 134 calibrate pH, 126 Log on, 109 fill the air trap, 134 UNICORN, 109 fill the optional filter, 137 flow path, 122 pH monitor, 124 replace a filter cassette, 122 Maintenance, 160 replace an ULTA filter, 123...
  • Page 204 Index Start, 109 User documentation, 8 Safety notices, 7 Safety precautions, 12 introduction, 12 valve usage statistics, 175 Sample application, 147 SNAP connectors, 33 Software, 49, 50 overview, 49 software modules, 49 System Control module, 50 Software overview, 48 Space requirements, 59 Standard instrument, 25, 26 Start a run, 150 Steel filter housing, 40...
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  • Page 206 © 2020 Cytiva All goods and services are sold subject to the terms and conditions of sale of the supplying company operating within the Cytiva business. A copy of those terms and conditions is available on request. Contact your local Cytiva representative for the most current information.

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