cytiva NanoAssemblr Ignite Operating Instructions Manual page 63

Syringe size
If the syringes for the C and R channels are the same size, use the start waste value
listed in the table below. If using dilution, add the value from the column Dilution
syringe. If using same size syringes for both channels, skip to the second table for
calculation recommendations.
Syringe size (mL)
Start waste recommendations for
NxGen 500 and NxGen 500D
Cartridges
The value for the parameter Start waste can be between 0 mL and the value of Total
volume.
The recommended values for the parameter Start waste can be calculated by
summing the recommended start waste values for specific syringe sizes.
Note:
NanoAssemblr Ignite and Ignite+ Operating Instructions NIN1134 AA
Values used to calculate Start waste for different sized
(mL)
Smaller syringe
1 0.10
3 0.15
5 0.20
10 0.30
20
30
Values used to calculate Start waste for same sized
Same size syringes
1
3
5
10
20
30
The following recommendations suggest collecting a larger amount of
waste to ensure the most uniform population of particles. If it is desirable to
collect less waste, optimize the parameter values by systematically
collecting less waste and testing each result. Contact us for further help
with parameter optimization. See
on page 119.
syringes (mL)
Larger syringe
N/A
0.35
0.50
0.80
-
-
syringes (mL)
0.3
0.50
0.70
1.10
-
-
Contact information for support,
5.1.3 Waste parameters
Dilution syringe
0.20
0.25
0.35
0.50
- 0.65
- 0.65
Dilution syringe
0.20
0.25
0.35
0.50
0.65
0.65
5 Preparation
5.1 Parameters
63