Magicprep Ngs Instrument Functional Test; Starting The Run; Quantify The Libraries; Specifications - tecan MagicPrep NGS Operating Manual

Problem / Error
Instrument hardware
problem
6.6

MagicPrep NGS Instrument Functional Test


The protocol below outlines the steps necessary to run a functional library preparation test of the
MagicPrep NGS system.

When setting up the MagicPrep NGS system for the first time, it is useful to perform a functional
test in addition to the hardware test to confirm performance and set a baseline for your system.

A functional test may also be advised after a system error where a hardware failure was not
detected.
Kit: Revelo DNA-Seq Enz for MagicPrep NGS
Input: 100 ng human genomic DNA (NA12878, Coriell (https://www.coriell.org), Cat #: GM12878)
Number of PCR cycles: 6

6.6.1 Starting the run:

1. Follow the instructions provided in the Revelo DNA-Seq Enz for MagicPrep NGS user guide.

6.6.2 Quantify the libraries:

1. Remove the libraries from the MagicPrep NGS instrument.
2. Measure the library yield using a Qubit. Follow the instructions for measuring DNA
concentration on a Qubit.

6.6.3 Specifications:


The total yield should be ≥ 200 ng.

The coefficient of variation (%CV) across the 8 samples should be ≤ 20%.
M01534_v1.1 MagicPrep NGS Instrument Operating Manual
Possible Cause
The temperature
measurement error with
the PCR/Thermal
module has occurred.
It is not possible to
continue the library
preparation.
%���� =
Corrective Measure
If the error still exists, please contact Tecan
Customer Support.
Stop the library run
Follow the on-screen instructions to remove
the used reagent cartridge and sample deck
 Switch off the instrument
 Switch on the instrument to initiate the self-
test/diagnostic sequence.
 Reload the MagicPrep NGS and try to
repeat the library preparation.
If the error still exists, please contact Tecan
Customer Support.
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Operating Manual
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