Sop-11: Laser Frequency Calibration Of The Ramanmicro 300 - PerkinElmer RamanMicro 300 Series Getting Started Manual

8. Select Wavelength (X-axis), and then click OK.
The instrument will now perform the wavelength calibration routine. This will take some
time.
After completing the wavelength calibration routine, a report is generated on-screen which
describes the result of the wavelength calibration.
The instrument is now wavelength calibrated.
NOTE: If the calibration fails, please contact your PerkinElmer Service Engineer.
SOP-11: Laser Frequency Calibration of the
RamanMicro 300
Laser frequency calibration should only be performed if the instrument has failed a
wavelength calibration verification.
1. Ensure the RamanMicro 300 is switched on by following SOP-1.
2. Select the 50x objective lens on the microscope.
3. Open the Scan and Instrument Setup dialog.
4. On the Hardware tab select Microscope 50x from the Beam path drop-down list.
5. Ensure that the laser power is set to 100%.
6. Click Spectral Preview in the Scan and Instrument Setup dialog, and focus on a
sample of polystyrene (L1181228).
When the polystyrene sample is optimally focused, close the Scan and Instrument Setup
dialog by clicking Exit.
7. Select the Dark Field (DF) switch position on the Olympus illuminator module.
8. Select Validate and then Instrument from the Instrument menu in Spectrum.
The Validation / Calibration dialog is displayed.
9. Select the Calibration tab.
10. Select Laser Frequency, and then click OK.
The instrument will now perform the laser frequency calibration routine. This will take a
few minutes.
After completing the laser frequency calibration routine, a report is generated on-screen
that gives details on the outcomes of the laser frequency calibration.
In the unlikely event that the laser frequency calibration routine should fail, please
perform a wavelength calibration and repeat the laser frequency calibration.
NOTE: If the calibration fails again, please contact your PerkinElmer Service Engineer.
The instrument in now laser frequency calibrated.
Using the RamanMicro 300 . 85