PerkinElmer RamanMicro 300 Series Getting Started Manual page 66

66 . RamanMicro 300 Getting Started Guide
5. Very slowly move one of the coupler knobs clockwise and counter-clockwise until the
counts level is maximized.
Figure 22 Adjustment of the XY Raman coupler
6. Move the other knob in a similar manner until the counts level has maximized.
7. Return to the first knob and move again to maximize the counts (the movement should
be much less than used the first time).
8. Return to second knob and do likewise.
9. Tighten each retaining screw a little and move the xy coupler knobs to compensate
each time.
10. Tighten screws until the plate at the rear coupler is secure – do not over tighten.
Aligning the Laser Path
The laser path only needs realignment if the beam is observed to move grossly in the xy
plane when moving into and out of focus. If the laser path is aligned correctly then the laser
spot should get larger in off ̵ focus positions without appreciable lateral movement.
NOTE: The requirement for laser alignment is NOT indicated by a situation where the laser
spot and the center of the crosshairs are non-coincident (this scenario requires
realignment of the video camera).
If the system is misaligned, we recommended that you contact a PerkinElmer Service
Engineer.
NOTE: Movement of the laser spot in the xy plane may be caused if the surface of the
sample is not flat or not mounted correctly. Ensure that the stage is level and that
the sample is flat. PerkinElmer recommends the use of a flat silicon surface.