Sop-4: Sample Positioning Using A Manual Stage - PerkinElmer RamanMicro 300 Series Getting Started Manual

SOP-4: Sample Positioning using a Manual Stage

NOTE: The RamanMicro 300 system can be purchased with either a manual stage or with a
motorized stage.
1. Lower the microscope sample stage.
2. Place the sample on a microscope slide and place it on the stage.
Figure 25 Positioning the sample under the objective lens
3. Select the 5x objective lens on the microscope.
4. Move the sample so that it is approximately lined up below the objective lens.
5. Select the Bright Field (BF) switch position on the Olympus illuminator module.
6. Switch on the illumination source.
The switch is located on the right side of the microscope frame.
7. Set the required light intensity using the adjustment knob on the right side of the
microscope frame base.
8. In Spectrum software, click
9. On the Hardware tab select Microscope 5x from the Beam path drop-down list.
10. Click to open the Alignment Camera window.
11. Focus on the sample using the microscope z-axis control knob.
Take care not to crush the sample. The working distances of the objective lenses
supplied as standard are 20 mm (5x), 1.3 mm (20x) and 0.38 mm (50x).
12. Accurately position the sample in the x and y directions using the stage control knobs.
Using the RamanMicro 300 . 73
to open the Scan and Instrument Setup dialog.
Place your
sample here