PerkinElmer RamanMicro 300 Series Getting Started Manual page 65

10. Loosen the 4 screws on the top plate of the Raman module until the camera is just free
to move (Figure 21).
Figure 21 Top view of the microscope showing the screws used to adjust the
position of the camera module
11. Move the camera module until the crosshairs line up as closely as possible with the
center of the laser spot.
12. Re-tighten the 4 screws.
13. Use the arrows in the Alignment Camera window to fine-tune the position of the
crosshairs to be in the center of the laser spot.
NOTE: If the video camera image is inverted, select Invert Image from the Camera menu.
14. If required, select Save Calibration from the Calibrate menu to save the new settings.
Aligning the Raman Coupler
You should only adjust the Raman coupler if the signal throughput has decreased. This may
occur if you have moved the unit. The only adjustment to the Raman is the xy-adjuster at
the rear of the Raman module. The procedure for alignment is as follows:
1. Place a flat sample of silicon on the sample stage.
We recommend that you purchase the sCAL Raman Calibration Standard (L1321843) for
this alignment procedure.
2. Loosen, but do not remove, the 4 retaining screws at the coupler so that about 0.5 mm
of thread is showing.
3. Using Video Preview in Spectrum, ensure that the sample is in focus and centered.
4. Using Spectral Preview note the maximum number of counts in the window.
Installing Your RamanMicro 300 . 65