PerkinElmer RamanMicro 300 Series Getting Started Manual page 75

Place your
sample here
Figure 26 Sample position on motorized stage
3. Select the 5x objective lens on the microscope.
4. Move the sample so that it is approximately lined up below the objective lens.
5. Select the Bright Field (BF) switch position on the Olympus illuminator module.
6. Switch on the illumination source.
7. Select Setup from the Instrument menu in Spectrum.
The Scan and Instrument Setup dialog is displayed.
8. On the Hardware tab select Microscope 5x from the Beam path drop-down list.
9. Click to open the Alignment Camera window.
10. Use the joystick to move the stage, or click Stage Control and then use the
appropriate fields in the Sample Position section to focus on the sample.
The joystick operates in the following manner:
Left / Right joystick movement gives x movement of the stage.
•
• Top / Bottom joystick movement gives y movement of the stage.
• Twisting the joystick gives z movement of the stage:
Left twist (counter clockwise) gives upward stage movement;
Right twist (clockwise) gives downward stage movement.
NOTE: You can also click Video Focus Now to perform an automatic video focus when you
are close to the sample surface.
Using the RamanMicro 300 . 75