Alignment Of The Microscope; Aligning The Microscope Objectives - PerkinElmer RamanMicro 300 Series Getting Started Manual

Alignment of the Microscope

Do not disconnect the microscope from the spectrometer. Only trained
PerkinElmer Service Engineers should attach and remove the fiber optic
cables that connect the two modules.
WARNING
The RamanMicro 300 system consists of three primary components:
The microscope;
•
The Raman module;
•
• The camera module.
Please read and understand the general safety precautions before proceeding. If you do not
understand any of the details regarding general safety precautions, please contact
PerkinElmer before performing any of the following procedures.

Aligning the Microscope Objectives

The microscope objective nosepiece can fit up to 4 objectives.
1. Select the highest magnification objective lens on the microscope (either the 100x or
50x).
2. Select the Bright Field (BF) switch position on the Olympus illuminator module.
3. Place a slide with an easily recognizable feature on it (for example, a graticule slide).
4. In the Spectrum software, select Instrument from the Setup menu or click
The Scan and Instrument Setup dialog is displayed.
5. Click Video Preview.
The Alignment Camera window opens.
6. Move the stage until the feature is in focus and in the center of the crosshairs in the
Alignment Camera window.
7. Select the next highest magnification objective.
8. Insert the adjuster keys supplied with the microscope into the nosepiece and move the
adjusters until the crosshairs are aligned with the same feature as for the previous
objective lens.
9. Repeat Steps 7–8 until all the objectives are aligned.
Installing Your RamanMicro 300 . 63
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