Zeiss Axioskop 40 Operating Manual
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Zeiss Axioskop 40 Operating Manual

Zeiss Axioskop 40 Operating Manual

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  • Page 2: Copyright

    Axioskop 40 Carl Zeiss Copyright Axioskop 40 FL Knowledge of this manual is required for the operation of the instrument. Would you therefore please make yourself familiar with the contents of this manual and pay special attention to hints concerning safe operation of the instrument.

  • Page 3: Table Of Contents

    Overall view of Axioskop 40 FL.................. 0-13 CHAPTER 1 INSTRUMENT DESCRIPTION ..................1-2 Name and intended application................... 1-2 Instrument description and main features..............1-2 System Overview Axioskop 40 / 40 FL................1-4 Objectives........................1-8 Eyepieces........................1-10 Stage micrometers and eyepiece reticules..............1-11 Technical data ......................1-13...

  • Page 4: Contents

    Changing the binocular tube ..................2-33 2.2.11 Attaching Axioskop connector plate................2-34 2.2.12 Installing and removing "Push&Click" modules on the Axioskop 40 FL......2-35 2.2.13 Changing the filter set in the FL P&C reflector module..........2-36 2.2.14 Changing the beam splitter in the FL P&C reflector module ........2-38 2.2.15...
  • Page 5 Axioskop 40 operation and function controls............... 3-4 Axioskop 40 FL operation and function controls ............3-10 Operation controls for accessory components............3-16 Switching Axioskop 40 and Axioskop 40 FL on and off ..........3-20 Illumination and contrast procedures................. 3-21 3.5.1 Setting transmitted-light bright field according to KÖHLER.........
  • Page 6 INTRODUCTION Axioskop 40 Carl Zeiss Contents Axioskop 40 FL Page CHAPTER 4 CARE, MAINTENANCE, TROUBLESHOOTING AND SERVICE ........4-2 Instrument care ......................4-2 Instrument maintenance ..................... 4-3 4.2.1 Performing checks .......................4-3 4.2.2 Changing fuses ......................4-3 Troubleshooting ......................4-4 Spares, consumables and tools ................... 4-8 Requesting service ......................

  • Page 7: Microscopy In A Few Steps

    KÖHLER" Please read the instructions regarding instrument safety and the Instrument Description (Chapter 1) and Start-Up (Chapter 2) chapters before using the Axioskop 40 or Axioskop 40 FL microscopes for the first time. As described in Chapter 2 (Start-Up), the microscope is ready for operation and switched on according to Section 3.4.

  • Page 8: Notes On Instrument Safety

    Axioskop 40 FL Notes on instrument safety The Axioskop 40 and Axioskop 40 FL microscopes have been designed, produced and tested in compliance with DIN 61010-1 (IEC 1010-1) safety requirements for electrical measuring, control and laboratory instruments, and meet the requirements of Appendix I of directive 73/23/EC.
  • Page 9 Axioskop 40 FL Notes on instrument safety Carl Zeiss The Axioskop 40 and Axioskop 40 FL microscopes, including original accessories, may only be used for the microscope techniques described in this manual. Particular attention must be paid to the following warnings: The manufacturer cannot assume any liability for any other applications, including individual modules or single parts.
  • Page 10 Notes on instrument safety Axioskop 40 FL The Axioskop 40 and Axioskop 40 FL microscopes are not equipped with any special devices for protection from substances that are corrosive, toxic, radioactive or other substances that could be hazardous to health. All the legal regulations, particularly the pertinent accident prevention regulations of the respective country, must be observed when handling such substances.

  • Page 11: Notes On Warranty

    This forfeits all warranty claims. With the exception of the work specified in this manual, no maintenance or repair of the Axioskop 40 / Axioskop 40 FL may be undertaken. Repairs may only be performed by Zeiss service staff or specially authorized personnel.

  • Page 12: Overall View Of Axioskop 40

    INTRODUCTION Axioskop 40 Carl Zeiss Overall view of Axioskop 40 Axioskop 40 FL Overall view of Axioskop 40 Eyepieces Binocular tube Stand Transmitted-light illuminator Condenser 0.9/1.25 H Mechanical stage Objective 0-12 B 40-810 e 12/01...

  • Page 13: Overall View Of Axioskop 40 Fl

    Axioskop 40 INTRODUCTION Axioskop 40 FL Overall view of Axioskop 40 FL Carl Zeiss Overall view of Axioskop 40 FL Eyepieces Ergo-tube 20°/23 with height adjustment Stand Incident-light illuminator Transmitted-light illuminator Universal condenser 0.9 Mechanical stage Objective B 40-810 e 12/01...
  • Page 14 INTRODUCTION Axioskop 40 Carl Zeiss Axioskop 40 FL 0-14 B 40-810 e 12/01...
  • Page 15 Table of contents INSTRUMENT DESCRIPTION..................1-2 Name and intended application ..................1-2 Instrument description and main features ................ 1-2 System Overview Axioskop 40 / 40 FL ................1-4 Objectives ........................1-8 Eyepieces ........................1-10 Stage micrometers and eyepiece reticules ..............1-11 Technical data .......................

  • Page 16: Name And Intended Application

    Manufacturer's designation: Axioskop 40 microscope Axioskop 40 FL microscope The Axioskop 40 and Axioskop 40 FL microscopes have been designed as universal microscopes for biomedical applications. They can be used as pure transmitted-light microscopes or, with epi- fluorescence equipment, as combined transmitted-light / reflected-light microscopes The Axioskop 40 and Axioskop 40 FL microscopes are mainly used in: −...
  • Page 17 Axioskop 40 FL Instrument description and main features Carl Zeiss Major instrument features of the Axioskop 40: − Stable and sturdy diecast stand with T-shaped base in pyramid design and integrated power supply for the 12 V, 35 W halogen lamp −...

  • Page 18: System Overview Axioskop 40 / 40 Fl

    INSTRUMENT DESCRIPTION Axioskop 40 Carl Zeiss System overview Axioskop 40 / 40 FL Axioskop 40 FL System Overview Axioskop 40 / 40 FL B 40-810 e 12/01...
  • Page 19 INSTRUMENT DESCRIPTION Axioskop 40 Axioskop 40 FL System overview Axioskop 40 / 40 FL Carl Zeiss B 40-810 e 12/01...
  • Page 20 INSTRUMENT DESCRIPTION Axioskop 40 Carl Zeiss System overview Axioskop 40 / 40 FL Axioskop 40 FL B 40-810 e 12/01...
  • Page 21 INSTRUMENT DESCRIPTION Axioskop 40 Axioskop 40 FL System overview Axioskop 40 / 40 FL Carl Zeiss B 40-810 e 12/01...

  • Page 22: Objectives

    Achroplan 10x/0.25 ∞/0.17 where 10x : objective magnification, with a defined color ring on the objective being allocated to each magnification step (Zeiss color code) 0.25 : numerical aperture ∞ : infinite image distance; these objectives...

  • Page 23: Instrument Description

    To prevent oil contamination of the specimen when the nosepiece is turned, the resilient mounts of the immersion objectives can be locked in their lifted position by turning them clockwise (do not forget to unlock them again!). The following objectives are ideal for the Axioskop 40 and Axioskop 40 FL microscopes: Microscopy technique Objective...

  • Page 24: Eyepieces

    440760-0000-000 Plan-Apochromat 100x/1.40 Oil 0.09 0.17 440780-0000-000 Eyepieces The following eyepieces are offered for the Axioskop 40 and Axioskop 40 FL: Eyepiece Image angle Application Cat. No. Eyepiece W-PL 10x/23 Br. foc. (2x) 49.4° with all tubes, see Pp. 1-4 ff.;...

  • Page 25: Stage Micrometers And Eyepiece Reticules

    INSTRUMENT DESCRIPTION Axioskop 40 Axioskop 40 FL Stage micrometers and eyepiece reticules Carl Zeiss Stage micrometers and eyepiece reticules Measuring and counting using a microscope requires stage micrometers and eyepiece reticules, a selection of which is listed below: Illustration Designation, technical data Cat.
  • Page 26 INSTRUMENT DESCRIPTION Axioskop 40 Carl Zeiss Stage micrometers and eyepiece reticules Axioskop 40 FL Illustration Designation, technical data Cat. No. Net micrometer 12.5x12.5/5;10 / d = 26 mm 474068-0000-000 area 12.5x12.5 mm, divided into 10x10 fields Photo reticule MC 2.5x / d = 26 mm 454075-0000-000 for 35 mm photography with 2.5x additional...

  • Page 27: Technical Data

    Technical data Dimensions (width x depth x height) Axioskop 40 stand................ approx. 280 mm x 560 mm x 505 mm Axioskop 40 FL stand with HBO 100 and adjustable ergo-tube 20°/23 in highest setting......approx. 280 mm x 730 mm x 555 mm Weight Axioskop 40 ........................
  • Page 28 Power consumption when HBO 103 is used ................. 260 VA Fuses in accordance with IEC 127 Axioskop 40 microscope stand ..................T 1 A/H, 5x20 mm Axioskop 40 FL microscope stand ................T 1 A/H, 5x20 mm HBO 50AC transformer mbq52ac-z ..............100 V, 127 V: 2x T 4 A ........................
  • Page 29 ..........for brightfield, darkfield ....................... for phase contrast 1, 2, 3 Objective change..............manually via 6-position nosepiece H, W 0.8" Change of reflector module (only with Axioskop 40 FL) ....manually via 5-position reflector turret B 40-810 e 12/01 1-15...
  • Page 30 INSTRUMENT DESCRIPTION Axioskop 40 Carl Zeiss Axioskop 40 FL 1-16 B 40-810 e 12/01...

  • Page 31: Start-Up

    Changing the binocular tube ..................2-33 2.2.11 Attaching Axioskop connector plate................2-34 2.2.12 Installing and removing "Push&Click" modules on the Axioskop 40 FL ......2-35 2.2.13 Changing the filter set in the FL P&C reflector module ........... 2-36 2.2.14 Changing the beam splitter in the FL P&C reflector module ..........2-38 2.2.15...

  • Page 32: List Of Illustrations

    START-UP Axioskop 40 Carl Zeiss List of illustrations Axioskop 40 FL List of illustrations Fig. 2-1 Setting up the microscope....................2-4 Fig. 2-2 Attaching the binocular tube................... 2-5 Fig. 2-3 Inserting eyepieces ......................2-5 Fig. 2-4 Inserting the eyepiece reticule ..................2-6 Fig.
  • Page 33 Axioskop 40 START-UP Axioskop 40 FL List of illustrations Carl Zeiss Fig. 2-42 Changing the filter set in the FL P&C reflector module ........... 2-36 Fig. 2-43 Installing the filter and beam splitter ................2-37 Fig. 2-44 Changing the beam splitter ................... 2-38 Fig.

  • Page 34: Initial Start-Up

    Axioskop 40 FL START-UP The Axioskop 40 / Axioskop 40 FL can be installed, converted and started up by the customer. However, it is also possible to have the microscope installed or converted by Zeiss service staff for an extra charge.

  • Page 35: Attaching Binocular Tube Or Phototube

    All the binocular tubes listed in the system overview (refer to Section 1.3) can be attached to the Axioskop 40 / Axioskop 40 FL stand as described below. • Undo hexagonal screw (2-2/3) using the SW3 ball-headed screwdriver. Remove dust caps (2-2/2) from the bottom of the tube and the dovetailed mount (2-2/5) on the stand.
  • Page 36 START-UP Axioskop 40 Carl Zeiss Initial start-up Axioskop 40 FL Inserting the eyepiece reticule The PL 10x/23 Br. foc. eyepieces are intended for use with eyepiece reticules. The slight image shift caused by the additional path through glass is taken into account on the...

  • Page 37: Setting Interpupillary Distance Of The Binocular Tube

    Axioskop 40 START-UP Axioskop 40 FL Initial start-up Carl Zeiss Inserting the fold-over eyecups The eyepieces have a rubber eyeglass protection ring to prevent scratches on spectacles. The eyepieces can be replaced with fold-over eyecups when required. • Remove the eyeglass protection ring (2-4/2) from the eyepieces and insert the eyecups (2-4/1).

  • Page 38: Mounting Objectives

    START-UP Axioskop 40 Carl Zeiss Initial start-up Axioskop 40 FL 2.1.6 Mounting objectives • Move the mechanical stage with stage carrier to the lower stop position. • Remove dust caps (2-7/2) from the respective openings on the nosepiece. • Remove objectives (2-7/4) from the case and...

  • Page 39: Inserting The Refill Set In The Specimen Marker

    Axioskop 40 START-UP Axioskop 40 FL Initial start-up Carl Zeiss • Simply reverse the process to remove the adapter ring. If the objective comes out without the adapter ring and the adapter ring is stuck in the nosepiece, then you must screw the objective back into the adapter ring so that both are tightly interlocked.

  • Page 40: Attaching The Universal Condenser

    Carl Zeiss Initial start-up Axioskop 40 FL Do not allow the writing surfaces of the marker insert to get wet or dirty. Before use, store a refill set (marker insert with cover) in its original packaging at 0 to 25 °C and with approx.

  • Page 41: Connecting To Power

    HBO 100 W transformer on and off • Switch the microscope on and off using power switch (2-10/1). • For the Axioskop 40 FL, switch the HBO 100 W transformer on and off using the power switch (2-11/1). Fig. 2-11...

  • Page 42: Inserting And Replacing The 12 V, 35 W Halogen Lamp

    START-UP Axioskop 40 Carl Zeiss Initial start-up Axioskop 40 FL 2.1.11 Inserting and replacing the 12 V, 35 W halogen lamp • Remove the power cable from the microscope. • Turn off the microscope and allow it to cool down for some 15 minutes.

  • Page 43: Hbo 103 Illuminator (Axioskop 40 Fl)

    Axioskop 40 START-UP Axioskop 40 FL Initial start-up Carl Zeiss 2.1.12 HBO 103 illuminator (Axioskop 40 FL) Inserting the HBO 103 W/2 mercury vapor short-arc lamp The HBO 103 illuminator and the HBO 103 W/2 mercury vapor short-arc lamp are delivered separately packaged for safety reasons.
  • Page 44 START-UP Axioskop 40 Carl Zeiss Initial start-up Axioskop 40 FL Adjusting the HBO 103 illuminator Adjustment of the HBO 103 illuminator described below performed steps: Rough adjustment and subsequently fine adjustment. The latter can be performed with or without the adjustment guide (2-16/2) on the incident-light tube (2-16/1).
  • Page 45 Axioskop 40 START-UP Axioskop 40 FL Initial start-up Carl Zeiss Fig. 2-18 HBO 103 focal spot before rough Fig. 2-19 HBO 103 focal spot after rough adjustment adjustment The adjusting screws (2-17/2 and 5) should not be changed during rough adjustment of the focal spots, because they influence the reflector setting in the HBO 103 illuminator.
  • Page 46 START-UP Axioskop 40 Carl Zeiss Initial start-up Axioskop 40 FL Fine adjustment without adjustment guide • Attach the HBO 103 illuminator to the microscope stand as described in Section 2.1.12 (2). • Remove one objective from the nosepiece and switch the empty opening into the function position.

  • Page 47: Hbo 50 Fluorescence Illuminator (Axioskop 40 Fl)

    • Place the HBO 50 fluorescence illuminator (2-21/3) on the connection tube (2-21/1) on the Axioskop 40 FL stand, align and secure it with clamping screw (2-21/2). • Connect the HBO 50 connection cable to the transformer and the power supply cable to a power outlet.
  • Page 48 Carl Zeiss Initial start-up Axioskop 40 FL • Focus the collector with knurled knob (2-22/4), so that the two arcs are focused clearly. • Use the adjusting screw (2-22/1) to set the burner in an axial direction to the reflection, so that both arcs appear the same size in the viewing window of the adjustment guide (refer to Fig.

  • Page 49: Attachments And Conversions

    Axioskop 40 START-UP Axioskop 40 FL Attachments and conversions Carl Zeiss Attachments and conversions This chapter only describes the steps required for attachments to and conversions of the microscope. The corresponding components must be reset to a functioning status as described in Chapters 2 and 3.

  • Page 50: Inserting Stops (Ph, Var) And Neutral Filter In Slider

    START-UP Axioskop 40 Carl Zeiss Attachments and conversions Axioskop 40 FL 2.2.2 Inserting stops (Ph, Var) neutral filter in slider • Undo the four slot screws (2-24/1) and remove cover (2-24/2). • Insert stops (2-24/3 or 10) into the left or right position with the metal facing down in the slider (2-24/5).

  • Page 51: Attaching Filter Mount

    Axioskop 40 START-UP Axioskop 40 FL Attachments and conversions Carl Zeiss 2.2.3 Attaching filter mount • If necessary, remove the polarizer or overview adapter from the condenser carrier. • Hold filter mount (2-25/5) parallel to the bottom of the condenser carrier (2-25/3) and...

  • Page 52: Attaching Overview Adapter

    START-UP Axioskop 40 Carl Zeiss Attachments and conversions Axioskop 40 FL 2.2.5 Attaching overview adapter • Where necessary, remove the filter mount or polarizer from the condenser carrier. • Hold overview adapter (2-27/4) parallel to the bottom of the condenser carrier (2-27/3) and...

  • Page 53: Changing The Hbo 103 W/2 Mercury Vapor Short-Arc Lamp

    Axioskop 40 START-UP Axioskop 40 FL Attachments and conversions Carl Zeiss 2.2.6 Changing the HBO 103 W/2 mercury vapor short-arc lamp Before opening the lamp housing, ensure that there is no connection to the electrical mains. HBO 103 W/2 lamps may only be changed after they have cooled down. Allow the HBO 103 microscope illuminator to cool down for some 15 minutes to avoid the risk of burns.
  • Page 54 START-UP Axioskop 40 Carl Zeiss Attachments and conversions Axioskop 40 FL • Use the SW 3 ball-headed screwdriver to undo clamping screw (2-29/2) and remove HBO 103 illuminator (2-29/3) from the incident-light tube (2-29/1) on the microscope stand. • Use focusing knob (2-30/3) to bring the collector to the frontmost position (in the direction of the light).
  • Page 55 Axioskop 40 START-UP Axioskop 40 FL Attachments and conversions Carl Zeiss • Clamp fixing screw (2-31/5) onto the heat sink. • Insert the new lamp (2-31/1) inside the heat sink with the spring lever (2-31/4) pressed down in the lamp holder (2-31/2) provided in the illuminator mount (2-31/3).

  • Page 56: Changing The Burner For The Hbo 50 Fluorescence Illuminator

    START-UP Axioskop 40 Carl Zeiss Attachments and conversions Axioskop 40 FL 2.2.7 Changing burner HBO 50 fluorescence illuminator • Turn off the HBO 50 transformer. Remove the HBO 50 plug from the transformer socket. Danger of getting burned! Allow lamp housing to cool down for at least 15 minutes.
  • Page 57 Axioskop 40 START-UP Axioskop 40 FL Attachments and conversions Carl Zeiss Avoid fingerprints on the burner’s glass parts. Where necessary, remove same immediately. • Press down spring lever (2-32/4) and insert the burner with the heat sink into lamp holder (2-32/5).
  • Page 58 Mechanical stage with torque setting The Axioskop 40 / 40 FL stand is delivered with a factory-installed mechanical stage with torque setting. The specimen holder with single-handed operation is attached to the standard mechanical stage (000000-1104-300). Travel range and torque for the x and y setting can be individually varied.

  • Page 59: Changing The Mechanical Stage

    Axioskop 40 START-UP Axioskop 40 FL Attachments and conversions Carl Zeiss 2.2.9 Changing the mechanical stage Mechanical stages can be bolted on or turned and centered depending on their type. The range of movement in the x direction is 75 mm and in the y direction 50 mm.
  • Page 60 START-UP Axioskop 40 Carl Zeiss Attachments and conversions Axioskop 40 FL • Press the stage to the front against the spring pin and lower towards the back of stage carrier (2-35/3), then release it. • Tighten screw cap (2-35/2). Centering the stage...
  • Page 61 Axioskop 40 START-UP Axioskop 40 FL Attachments and conversions Carl Zeiss Adjusting the travel range on the ergo- drive On mechanical stages with ergo-drive, the travel range of x and y adjustment can be extended by a max. 15 mm by axial movement of the drive knobs.
  • Page 62 Axioskop 40 Carl Zeiss Attachments and conversions Axioskop 40 FL Adjusting the y direction • Hold the y drive knob (2-37/7) and turn the light knurled sleeve (2-37/8) above it clockwise right (sluggish action) or counterclockwise (easy action) until the desired torque is achieved.

  • Page 63: Changing The Binocular Tube

    Axioskop 40 START-UP Axioskop 40 FL Attachments and conversions Carl Zeiss 2.2.10 Changing the binocular tube • Use an SW 3 ball-headed screwdriver to undo clamping screw (2-39/3) and remove tube (2-39/1) upwards. • Place dust cap (2-39/2) for tube lens protection on the dovetail of the binocular tube.

  • Page 64: Attaching Axioskop Connector Plate

    The Axioskop connector plate is used to attach intermediate tubes and/or tubes from Axioplan 2 to the Axioskop 40 / Axioskop 40 FL. • Use a SW 3 ball-headed screwdriver to undo clamping screw (2-40/5) and remove the Axioskop 40 / 40 FL tube upwards.

  • Page 65: Installing And Removing "Push&Click" Modules On The Axioskop 40 Fl

    Carl Zeiss 2.2.12 Installing and removing "Push&Click" modules on the Axioskop 40 FL The FL P&C reflector modules and optovar and analyzer modules can only be installed on the Axioskop 40 FL, since its stand is equipped with a reflector turret. The following reflector modules can be installed: FL P&C reflector module...

  • Page 66: Changing The Filter Set In The Fl P&C Reflector Module

    Filter sets or fully assembled FL P&C reflector modules can be ordered from Carl Zeiss. • Remove FL P&C reflector module (2-42/3) from the reflector turret and set it down (also refer to Section 2.2.12).
  • Page 67 Axioskop 40 START-UP Axioskop 40 FL Attachments and conversions Carl Zeiss If it is necessary to install filters without direction arrows, we recommend the following procedure: Filters with reflective, dielectric layers need to be installed, so that the reflective layer (2-43/6) on the excitation filter (2-43/5) points outwards (in relation to the reflector module).

  • Page 68: Changing The Beam Splitter In The Fl P&C Reflector Module

    START-UP Axioskop 40 Carl Zeiss Attachments and conversions Axioskop 40 FL 2.2.14 Changing the beam splitter in the FL P&C reflector module Attachment of filters and the beam splitter requires utmost care to prevent damage to and contamination of the optical components.
  • Page 69 Axioskop 40 START-UP Axioskop 40 FL Attachments and conversions Carl Zeiss The reflective (layered) side (2-46/3) of the beam splitter has a slanted edge (2-46/1) or corner (2-46/2). • Place the excitation half of the module (2-45/1) onto the emission half of the module (2-45/4) (the retaining pins 2-45/5b will grip the eyelets 2-45/5a).

  • Page 70: Attaching The Active Co-Observation Equipment For 2 Users

    Attaching the active co-observation equipment for 2 users The active co-observation equipment for 2 users can be attached to the Axioskop 40 stand without restrictions. For the Axioskop 40 FL stand, the incident-light illuminator (e.g. HBO 103) must first be removed.
  • Page 71 Axioskop 40 START-UP Axioskop 40 FL Attachments and conversions Carl Zeiss Ergonomic binocular phototube Binocular tube Dust cap Rear dovetail Socket for power unit Heat protection shield Fastening screw Clamping screw Support point (stand) Power unit Plug Clamping screw Dovetail (stand)
  • Page 72 2.2.16 Attaching the multi-observation equipment We recommend that you request Zeiss Microscopy Service to assemble the multi-observation equipment (refer to Chapter 4), since careful optical adjustment and centering of the fields of view of the various observation tubes is required.

  • Page 73: Attaching The Multi-Observation Equipment

    Axioskop 40 START-UP Axioskop 40 FL Attachments and conversions Carl Zeiss The binocular tubes of the multi-observation equipment can be turned and fixed in position as required by the user. The multi-observation equipment with light pointer enables up to 9 observation stations to be set up with sufficient brightness for transmitted-light, bright-field.

  • Page 74: Attaching The Intermediate Tube For Image Projection

    • Use an SW 3 ball-headed screwdriver to undo clamping screw (2-49/8) and remove the tube on the Axioskop 40 / 40 FL upwards. • Insert the Axioskop connector plate (2-49/6) with the dovetail on the bottom into the stand opening (2-49/9) and slightly tighten clamping screw (2-49/8).
  • Page 75 Axioskop 40 operation and function controls ..............3-4 Axioskop 40 FL operation and function controls ............. 3-10 Operation controls for accessory components ..............3-16 Switching Axioskop 40 and Axioskop 40 FL on and off........... 3-20 Illumination and contrast procedures ................3-21 3.5.1 Setting transmitted-light bright field according to KÖHLER ..........
  • Page 76 Centering dark field stop on the universal condenser ............3-28 Fig. 3-24 Condenser 0.9/1.25 H and slider with phase stops............3-29 Fig. 3-25 Transmitted-light polarization components on the Axioskop 40 / 40 FL......3-30 Fig. 3-26 Determining the vibration direction n ' using the example of an artificial fiber ....3-31 γ...
  • Page 77 Axioskop 40 OPERATION Axioskop 40 FL Carl Zeiss B 40-810 e 12/01...

  • Page 78: Axioskop 40 Operation And Function Controls

    OPERATION Axioskop 40 Carl Zeiss Axioskop 40 operation and function controls Axioskop 40 FL OPERATION Axioskop 40 operation and function controls Fig. 3-1 Axioskop 40 operation and function controls B 40-810 e 12/01...

  • Page 79: Eyepieces

    Axioskop 40 OPERATION Axioskop 40 FL Axioskop 40 operation and function controls Carl Zeiss Key to Fig. 3-1: Eyepieces Binocular tube Light intensity regulator On / Off switch with integrated control display Focusing drive - rough adjustment (on both sides)
  • Page 80 OPERATION Axioskop 40 Carl Zeiss Axioskop 40 operation and function controls Axioskop 40 FL Color temperature key 3200 K (3-1/7) (3-1/7) − With the conversion filter switched on and full lamp voltage (12 V), the color temperature comes to 3200 K. This is required for photomicrography using color films (artificial light).
  • Page 81 Axioskop 40 OPERATION Axioskop 40 FL Axioskop 40 operation and function controls Carl Zeiss Luminous field diaphragm (3-1/12) − Wheel (3-2/2) for infinitely variable setting of luminous-field diaphragm (transmitted light). − Additional mount (3-2/1) for filters with diameter d = 32 mm.
  • Page 82 OPERATION Axioskop 40 Carl Zeiss Axioskop 40 operation and function controls Axioskop 40 FL Mechanical stage with torque setting and specimen holder (3-1/14) − Mechanical stage (3-4/2) placement, positioning and fastening of specimens with the specimen holder (3-4/1). Fig. 3-4...
  • Page 83 Axioskop 40 OPERATION Axioskop 40 FL Axioskop 40 operation and function controls Carl Zeiss Specimen marker The specimen marker is screwed into the nosepiece instead of an objective and can be used to mark details of special interest in color on the carrier plate.

  • Page 84: Axioskop 40 Fl Operation And Function Controls

    OPERATION Axioskop 40 Carl Zeiss Axioskop 40 FL operation and function controls Axioskop 40 FL Axioskop 40 FL operation and function controls Fig. 3-7 Axioskop 40 FL operation and function controls 3-10 B 40-810 e 12/01...
  • Page 85 Axioskop 40 OPERATION Axioskop 40 FL Axioskop 40 FL operation and function controls Carl Zeiss Key to Fig. 3-7: Eyepieces Ergo-tube 20°/23 with height adjustment Centering screws for luminous-field diaphragm Push rod for luminous-field diaphragm (incident light) Compartment for filter slider...
  • Page 86 OPERATION Axioskop 40 Carl Zeiss Axioskop 40 FL operation and function controls Axioskop 40 FL Compartment for filter slider (3-7/5) − For 3-position or 6-position filter slider with 18 mm filter diameter. Centering screws for aperture diaphragm (incident light) (3-7/6) −...
  • Page 87 Axioskop 40 OPERATION Axioskop 40 FL Axioskop 40 FL operation and function controls Carl Zeiss Drive knob for movement of the mechanical stage in x the direction (3-7/15) − Movement of the mechanical stage in the x direction, maximum travel range: 75 mm −...
  • Page 88 OPERATION Axioskop 40 Carl Zeiss Axioskop 40 FL operation and function controls Axioskop 40 FL Mechanical stage with torque setting and specimen holder (3-7/19)) − Mechanical stage (3-9/2) placement, positioning and fastening of specimens with the specimen holder (3-9/1). Fig. 3-9...
  • Page 89 Axioskop 40 OPERATION Axioskop 40 FL Axioskop 40 FL operation and function controls Carl Zeiss Luminous-field diaphragm (3-7/22) See page 3-7. Drive knob for condenser height adjustment (3-7/23) − Height adjustment of the condenser to set KÖHLER illumination. Clamping lever for focusing drive height stop (3-7/24) −...

  • Page 90: Operation Controls For Accessory Components

    − Push rod (3-12/1) pulled out: 100% to the photo port. The maximum weight allowed on the camera port for camera and cable is 2.5 kg. The Carl Zeiss Service can set a higher maximum load capacity (at a charge) on request.
  • Page 91 Axioskop 40 OPERATION Axioskop 40 FL Operation controls for accessory components Carl Zeiss Ergonomic binocular phototube 6-25°/23 (100/100) The camera port (3-13/1) of the ergonomic binocular phototube enables SLR-cameras, microscope cameras and video cameras to be attached via the corresponding adapters. The light can be directed either to the eyepieces or to the attached camera via a push rod.
  • Page 92 OPERATION Axioskop 40 Carl Zeiss Operation controls for accessory components Axioskop 40 FL Overview adapter 2.5x-4x The overview adapter makes for complete image field illumination when a low magnification objective is used (2.5x-4x) combined with the 0.9/1.25 H condenser (000000-1104-285).
  • Page 93 When several filters are combined, the transmission value is calculated as a percentage of the product of the individual transmission values. Filter slider (Axioskop 40 FL only) The 3-position or 6-position FL filter slider permits 18 mm diameter filters (e.g. PINKEL-type filters) to be directed into the incident-light beam path.

  • Page 94: Switching Axioskop 40 And Axioskop 40 Fl On And Off

    OPERATION Axioskop 40 Carl Zeiss Switching Axioskop 40 and Axioskop 40 FL on and off Axioskop 40 FL Switching Axioskop Axioskop 40 FL on and off Switching on • Switch the instrument on with the on / off switch on the side of the instrument (3-17/1).

  • Page 95: Illumination And Contrast Procedures

    KÖHLER illumination. These basic rules of microscope setting are described in detail in Section 3.5.1 (3) "Transmitted-light bright field settings according to KÖHLER on the Axioskop 40 and Axioskop 40 FL".
  • Page 96 (3-18/E). In most applications, this aperture diaphragm setting provides optimum contrast at almost Fig. 3-18 Axioskop 40 microscope settings for ideal resolution, and is therefore the best transmitted-light bright field compromise for the human eye.
  • Page 97 Axioskop 40 OPERATION Axioskop 40 FL Illumination and contrast procedures Carl Zeiss Setting height stop condenser carrier • Undo the fastening screw of height stop (3-19/1) using an SW 3 ball-headed screwdriver. • Use focusing drive to focus on the specimen.

  • Page 98: Setting Transmitted-Light Phase Contrast

    - depending on the phase position. This interference results in image contents displaying distinctions in intensity and color that can be perceived by the human eye. Axioskop 40 and Axioskop 40 FL configurations − Phase-contrast objectives with phase rings Ph 1, Ph 2 or Ph 3 for different average numerical apertures that can also be used in bright field without restriction.
  • Page 99 Illumination and contrast procedures Carl Zeiss Setting transmitted-light phase contrast on the Axioskop 40 and Axioskop 40 FL • Swivel phase-contrast objective, e.g. designated with Ph 1, into the beam path. • Insert phase stop labeled the same as the phase-contrast objective, e.g.

  • Page 100: Setting Transmitted-Light Varel Contrast

    This gives a richer contrast, relief-like image. Transmitted-light dark field components for Axioskop 40 and Axioskop 40 FL − 0.9/1.25 H condenser with slider, equipped with VAREL segment stops Var 1 / Var 2 −...

  • Page 101: Setting Transmitted-Light Dark Field

    Transmitted-light dark field configuration for the Axioskop 40 and Axioskop 40 FL − Universal condenser with dark field stop in position D and 0.76 to 0.90 aperture on the illumination side −...
  • Page 102 OPERATION Axioskop 40 Carl Zeiss Illumination and contrast procedures Axioskop 40 FL • Replace eyepiece in the tube. • If the height of the dark field condenser has been correctly enough, visible brightening in the field of view can be reduced, and the luminous-field diaphragm image will be almost perfectly in focus.

  • Page 103: Setting Transmitted-Light Oblique Illumination

    Oblique illumination allows light into the objective from one side only, which results in a relief-like image. Axioskop 40 and Axioskop 40 FL configuration for oblique illumination − 0.9/1.25 H condenser with slider, equipped preferably with phase stops Ph 1 / Ph 2 or Ph 1 / Ph 3 with stop Ph 1 in adjacent slider position to the soft stop notches.

  • Page 104: Setting Transmitted-Light Polarization

    • Insert analyzer carrier (3-25/1) above the nosepiece or swivel in analyzer module on reflector turret (Axioskop 40 FL). The field of view now appears dark on account of the crossed polarizers. 3-30 B 40-810 e 12/01...
  • Page 105 − Rotary mechanical stage 75x50/240° R (3-25/3) − Polarizer D (rotary or fixed), unless a condenser with integrated polarizer is used. − Analyzer carrier or analyzer module D in the reflector turret (only available with Axioskop 40 FL) − Adjusting specimen for polarization microscopy (453679-0000-000)
  • Page 106 (diagonal position). In this position, the specimen may display any color. • Push in compensator λ (only in Axioskop 40 FL because the opening is otherwise occupied by the analyzer slider). Like the specimen, compensator λ is a birefringent object, though with a defined path difference of 550 nm and the greatest vibration direction n γ...
  • Page 107 Axioskop 40 OPERATION Axioskop 40 FL Illumination and contrast procedures Carl Zeiss The path difference results from the superimposition (interference) of the vibration direction of the specimen and that of compensator λ. The greater path difference occurs if the vibration direction of the specimen with the absolutely or relatively highest refractive index (n γ...

  • Page 108: Setting Epi-Fluorescence

    If required, re-adjustment must be performed depending on the operation time. Setting epi-fluorescence on the Axioskop 40 FL The first epi-fluorescence setting is considerably simplified if the Plan-Neofluar objective 20x/0.50 and a specimen of pronounced fluorescence is used. It is also possible to use demonstration specimens first.
  • Page 109 Axioskop 40 OPERATION Axioskop 40 FL Illumination and contrast procedures Carl Zeiss • Switch on the HBO 103 or HBO 50 mercury vapor short-arc lamp (3-28/2) and allow it to warm up to operation temperature for some 15 minutes. • Select the FL P&C reflector module in the...

  • Page 110: Documentation

    3.6.1 Attaching photomicrography equipment The Axioskop 40 / Axioskop 40 FL microscope equipped with a binocular phototube (3-29/5) can be switched from observation to photomicrography using the push rod (3-29/7) (push rod pulled out). The special T2 adapter 2.5x and other T2 adapters enable commercially available 35 mm SLR cameras (SLR - single lens reflex) and special microscope cameras (e.g.
  • Page 111 Axioskop 40 OPERATION Axioskop 40 FL Documentation Carl Zeiss Attaching an SLR camera, e.g. CONTAX 167 ARIA • Screw T-2 adapter (416010-0000-000, 3-29/3) for the camera used onto the 2.5x connector for T2 (456005-0000-000, 3-29/4). • Attach camera (416181-0000-000, 3-29/2) and, if necessary, the shutter release cable (416167-0000-000, 3-29/1).
  • Page 112 MC 200 , from Carl Zeiss CHIP The Axioskop 40 / Axioskop 40 FL microscope allows not only attachment of SLR cameras, but also of microscope cameras, e.g. MC 80 or MC 200 from Carl Zeiss. You can order the MC 80 CHIP MC 200 CHIP microscope cameras directly from Carl Zeiss.
  • Page 113 Axioskop 40 OPERATION Axioskop 40 FL Documentation Carl Zeiss Various T2 adapters for SLR camera housings are listed below: T2 adapters for SLR camera housings Cat. No. T2 adapter for CONTAX (CONTAX bayonet) 416010-0000-000 T2 adapter for OLYMPUS OM (OM bayonet)

  • Page 114: Attaching Adapters For Video Cameras

    The following video adapters with 60 mm interface enable 1-chip b/w and color CCD cameras and 3- chip color/high-resolution CCD cameras to be connected to the phototube of the Axioskop 40 / 40 FL. Tube Adapters...
  • Page 115 Axioskop 40 FL Documentation Carl Zeiss The 60 - 44 adapter also allows video adapters with 44 mm interface to be used with the phototube of the Axioskop 40 / 40 FL with 60 mm interface. Video adapter Suitable for Comments (Cat.

  • Page 116: Attaching Adapters For Compact Digital Cameras

    OPERATION Axioskop 40 Carl Zeiss Documentation Axioskop 40 FL 3.6.3 Attaching adapters for compact digital cameras Tube Adapters Cameras 000000-1096-522 000000-1108-984 HOTO OMPACT TUBE DIGITAL M52x0.75 M52x0.75 CAMERAS SKOP CAMCORDER To be used with: To be used with: WITH WITH...

  • Page 117: Digital Cameras With 37 Mm Filter Thread, E.g. Sony Dcr-Pc100 / Sony Dsc-S50

    Axioskop 40 OPERATION Axioskop 40 FL Documentation Carl Zeiss 3.6.4 Digital cameras with 37 mm filter thread, e.g. SONY DCR-PC100 / SONY DSC-S50 When delivered, the sliding mount with M37 thread (3-31/2) and the lens mount (3-31/4) are pe-assembled. The drawing alongside shows the disassembled components.

  • Page 118: Digital Cameras With 52 Mm Filter Thread, E.g. Sony Dsc-S70, Dsc-S75, Dsc-S85

    • Tighten grub screw (3-31/3) again. If you use a camera adapter combination not expressly recommended by Carl Zeiss, it is possible that you will be unable to generate an untrimmed image. 3.6.5 Digital cameras with 52 mm filter thread, e.g.
  • Page 119 Perform the test until the image fills the format without trimming or vignetting. • Tighten grub screw (3-32/5) again. If you use a camera adapter combination not expressly recommended by Carl Zeiss, it is possible that you will be unable to generate an untrimmed image. B 40-810 e 12/01...

  • Page 120: Adapting Cameras With Different Filter Thread Sizes

    OPERATION Axioskop 40 Carl Zeiss Documentation Axioskop 40 FL 3.6.6 Adapting cameras with different filter thread sizes Cameras with a filter thread other than M37 or M52 can also be connected with filter adapters or reduction rings available in specialty photo stores. As mentioned above, the compatibility of the cameras and digital adapters can be determined only by testing them.

  • Page 121: Multiple Observer Equipment

    Axioskop 40 OPERATION Axioskop 40 FL Multiple observer equipment Carl Zeiss Multiple observer equipment 3.7.1 Using active co-observation equipment for 2 users The active co-observation equipment is designed for two users and consists of the following: − Basic unit (3-33/3) with two mounts for the binocular tubes −...
  • Page 122 OPERATION Axioskop 40 Carl Zeiss Multiple observer equipment Axioskop 40 FL Ergonomic binocular phototube Binocular tube Basic unit Power unit Adjusting knob for brightness control Lever for light pointer Fig. 3-33 Implementing active co-observation equipment for two users 3-48 B 40-810 e 12/01...

  • Page 123: Using Multi-Observation Equipment

    Focus can be corrected via the diopter setting of the foc. eyepieces both on the tube of the Axioskop 40 / 40 FL as well as on all the other co-observation tubes. Positioning of the light pointer and its brightness control can be performed on the basic unit of the multi-observation equipment.
  • Page 124 OPERATION Axioskop 40 Carl Zeiss Multiple observer equipment Axioskop 40 FL Adjusting knob for brightness control Co-observation tube Basic unit End connector L Lever for light pointer Power unit Binocular tube End connector R Connecting piece L Connecting piece R Co-observation tube Fig.

  • Page 125: Drawing Device

    Attaching switchable intermediate tube for image projection The intermediate tube for image projection (3-35/1) can also be attached to the new Axioskop 40 and Axioskop 40 FL routine micro- scopes via the connector plate (3-35/2). See section 2.2.17 for startup.

  • Page 126: Measuring Lengths

    Axioskop 40 FL Measuring lengths Measurement of lengths using the Axioskop 40 / 40 FL requires the following: − stage micrometer, positive 5 + 100/100 y, D = 0.17 mm as the specimen − eyepiece crossline micrometer 10:100, d = 26 mm in the eyepiece An overview of available stage micrometers and eyepiece reticules is available in Chapter 1.
  • Page 127 Axioskop 40 OPERATION Axioskop 40 FL Measuring lengths Carl Zeiss After the stage micrometer has been exchanged for the specimen to be measured, the measuring distance of interest results from the number of increments of the crossline micrometer (tenth estimated), multiplied by the scale value k’.
  • Page 128 OPERATION Axioskop 40 Carl Zeiss Axioskop 40 FL 3-54 B 40-810 e 12/01...

  • Page 129: Care, Maintenance, Troubleshooting And Service

    Axioskop 40 CARE, MAINTENANCE, TROUBLESHOOTING AND SERVICE Axioskop 40 FL Table of contents / List of illustrations Carl Zeiss CARE, MAINTENANCE, TROUBLESHOOTING AND SERVICE Table of contents CARE, MAINTENANCE, TROUBLESHOOTING AND SERVICE ........4-2 Instrument care ....................... 4-2 Instrument maintenance....................4-3 4.2.1...

  • Page 130: Instrument Care

    CARE, MAINTENANCE, TROUBLESHOOTING AND SERVICE Instrument care Care of the Axioskop 40 / Axioskop 40 FL is limited to the following operations: • Switch off the instrument each time after use and place the instrument cover on it to protect it from dust and humidity.

  • Page 131: Instrument Maintenance

    Axioskop 40 CARE, MAINTENANCE, TROUBLESHOOTING AND SERVICE Axioskop 40 FL Instrument maintenance Carl Zeiss Instrument maintenance 4.2.1 Performing checks • Take sure to ensure that the instrument complies with the required line voltage (e.g. on the HBO 100 transformer). • Check the power cable and plug for defects.

  • Page 132: Troubleshooting

    CARE, MAINTENANCE, TROUBLESHOOTING AND SERVICE Axioskop 40 Carl Zeiss Troubleshooting Axioskop 40 FL Troubleshooting Problem Cause Remedy Shadows or inhomogeneous The vis/fot push rod on the camera Move the vis/fot push rod on the image brightness in the field of...
  • Page 133 Axioskop 40 CARE, MAINTENANCE, TROUBLESHOOTING AND SERVICE Axioskop 40 FL Troubleshooting Carl Zeiss Problem Cause Remedy Low resolving power and poor The aperture diaphragm opening Set the aperture diaphragm image contrast. is not set correctly. opening as per the 2/3 rule or the specimen features, see P.
  • Page 134 CARE, MAINTENANCE, TROUBLESHOOTING AND SERVICE Axioskop 40 Carl Zeiss Troubleshooting Axioskop 40 FL Problem Cause Remedy Greater focus differences after The focusing eyepieces are not set Set the focusing eyepieces to the objective change. correctly. appropriate ametropia, s. P. 2-6.
  • Page 135 Axioskop 40 CARE, MAINTENANCE, TROUBLESHOOTING AND SERVICE Axioskop 40 FL Troubleshooting Carl Zeiss Problem Cause Remedy The 12 V 35 W halogen lamp The power plug is not connected Connect the power plug to the does not function although the to the power outlet.

  • Page 136: Spares, Consumables And Tools

    CARE, MAINTENANCE, TROUBLESHOOTING AND SERVICE Axioskop 40 Carl Zeiss Spares, consumables and tools Axioskop 40 FL Spares, consumables and tools Description Cat. No. Application 12 V 35 W halogen lamp 000000-0425-360 for transmitted-light illumination HBO 50AC mercury vapor short-arc lamp...

  • Page 137: Requesting Service

    Requesting service All repairs of mechanical, optical or electronic components inside the instrument and of the electrical components of the Axioskop 40 / Axioskop 40 FL may only be performed by Carl Zeiss service staff or specially authorized personnel. To ensure optimum setting and trouble-free function of your microscope over a longer period of time, we recommend that you enter into a service/maintenance agreement with Carl Zeiss.
  • Page 138 CARE, MAINTENANCE, TROUBLESHOOTING AND SERVICE Axioskop 40 Carl Zeiss Axioskop 40 FL 4-10 B 40-810 e 12/01...

  • Page 139: Appendix

    Axioskop 40 APPENDIX Axioskop 40 FL Overview Carl Zeiss APPENDIX List of abbreviations...........................A-2 Index..............................A-4 Certification in accordance with DIN ISO 9001 / EN 46001 EC conformity declaration B 40-810 e 12/01...

  • Page 140: List Of Abbreviations

    APPENDIX Axioskop 40 Carl Zeiss List of abbreviations Axioskop 40 FL List of abbreviations Alternating Current A-Plan Achromatic objectives featuring improved image flatness (ICS optics line) Suitable for eyeglass wearers Charge Coupled Device cod. Coded Canadian Standards Association Cover slip thickness Dark field Diameter (e.g.
  • Page 141 Axioskop 40 APPENDIX Axioskop 40 FL List of abbreviations Carl Zeiss Infinity Color-Corrected System International Electrical Engineering Commission International Protection (protection class) International Organization for Standardization Measuring distance (length measurement) Light Emitting Diode Microscope Camera MOT / mot. Motorized Refractive index of the medium...

  • Page 142: Index

    APPENDIX Axioskop 40 Carl Zeiss Index Axioskop 40 FL Index Page Adapter ............................3-40 Adjustment guide ......................2-15, 3-11, 3-12 Ambient conditions .........................1-13 Ametropia ............................2-6 Analyzer ............................3-30 Analyzer module ........................3-14, 3-30 Analyzer slider ..........................3-30 Aperture diaphragm ..................3-7, 3-12, 3-13,3-22, 3-35 Application ............................1-2...
  • Page 143 Axioskop 40 APPENDIX Axioskop 40 FL Index Carl Zeiss Epi-fluorescence ........................1-2, 3-36 Ergo-drive ............................2-31 Ergonomic binocular phototube ...................... 3-17 Ergo-tube ..........................3-11, 3-16 Eyecups ............................2-7 Eyepieces ......................1-10, 2-5, 2-6, 3-5, 3-11 Eyepiece reticle ..........................2-6 Filter ....................3-5, 3-6, 3-7, 3-11, 3-13, 3-18, 3-36 Filter mount .........................
  • Page 144 APPENDIX Axioskop 40 Carl Zeiss Index Axioskop 40 FL Light intensity ........................ 3-5, 3-11, 3-12 Light sources ...........................1-14 Line voltage .......................... 1-13, 1-14 Line connection ..........................2-11 Luminous-field diaphragm ............3-5, 3-7, 3-11, 3-15, 3-22, 3-25, 3-35 Maintenance .............................4-3 Measurement of lengths .........................3-52 Mechanical stage ............2-28, 2-29, 2-32, 3-5, 3-6, 3-8, 3-11, 3-12, 3-14...
  • Page 145 Axioskop 40 APPENDIX Axioskop 40 FL Index Carl Zeiss Spares .............................. 4-8 Specimen holder .....................2-32, 3-5, 3-8, 3-11, 3-14 Specimen marker ......................2-9, 3-9, 3-14 Stage carrier ......................2-10, 2-19, 2-29, 2-30 Stage focusing ......................... 3-9, 3-15 Stage micrometer ........................... 1-11 Start-up .............................
  • Page 146 APPENDIX Axioskop 40 Carl Zeiss Axioskop 40 FL B 40-810 e 12/01...

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