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MiSeq System Guide
Measure Expected Wash Volumes
Measuring expected wash volumes confirms that wash fluidics are performing efficiently.
1
Before beginning a wash, empty the waste bottle.
2
When the wash is complete, measure the wash volume in the waste bottle.
Wash Type
Post Run Wash
Post Run Wash that includes a Template Line Wash
Standby Wash
Maintenance Wash
Resolve Reagent Chiller Temperature Errors
The required temperature range of the reagent chiller is 2°C to 11°C. A sensor indicator shows the
temperature of the reagent chiller. See
If you receive an error message that the chiller is not in the specified temperature range, contact Illumina
Technical Support.
For more information on the reagent chiller, see
Resolve Local Run Manager Analysis Errors
For troubleshooting information related to analysis errors, see the Local Run Manager Software Guide
(document # 1000000002702) . It includes instructions on how to re-queue analysis.
Configure System Settings
The MCS includes several screens that access commands to configure the system. Typically software
settings are configured during MiSeq installation.
Configure IP and DNS Settings
Configure IP address and DNS server addresses if required due to a network or facility change.
1
From the Home screen, select Manage Instrument.
2
Select System Settings.
Select Obtain an IP address automatically or Use the following IP address.
u
If you select Use the following IP address, enter an IP address, subnet mask, and default gateway.
Select Obtain DNS address automatically or Use the following DNS server addresses.
u
If you select Use the following DNS server addresses, enter a preferred and alternate DNS server
address.
3
Select Save and Continue.
Document # 15027617 v04Material # 20024228
For Research Use Only. Not for use in diagnostic procedures.
Expected Wash Volume
17.25 ml
25.5 ml
46 ml
51.75 ml
Sensor Indicators on page
Reagent Compartment on page
6.
3.
46
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