Automatic Post-Run Wash - illumina NextSeq 550Dx Instrument Reference Manual

NextSeq 550Dx Research Mode Instrument Reference Guide
Sequencing Analysis Viewer
The Sequencing Analysis Viewer software shows sequencing metrics generated during the run. Metrics
appear in the form of plots, graphs, and tables based on data generated by RTA and written to InterOp
files. Metrics are updated as the run progresses. Select Refresh at any time during the run to view
updated metrics. For more information, see the Sequencing Analysis Viewer User Guide (part #
15020619) .
The Sequencing Analysis Viewer is included in the software installed on the instrument computer. You
can also install Sequencing Analysis Viewer on another computer linked to the same network as the
instrument to monitor run metrics remotely.

Automatic Post-Run Wash

When the sequencing run is complete, the software initiates an automatic post-run wash using the wash
solution provided in the buffer cartridge and NaOCl provided in the reagent cartridge. If the option purge
consumables for the run is enabled, the purge occurs before the automatic post-run wash.
The automatic post-run wash takes approximately 90 minutes. When the wash is complete, the Home
button becomes active. Sequencing results remain visible on the screen during the wash.
After the Wash
After the wash, the sippers remain in the down position to prevent air from entering the system. Leave
the cartridges in place until the next run.
Document # 1000000041922 v01
For Research Use Only. Not for use in diagnostic procedures.
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