Inspect The Reagent Cartridge; Denature And Dilute Libraries; Load Sample Libraries - illumina MiSeq System Manual

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MiSeq System Guide

Inspect the Reagent Cartridge

Invert the reagent cartridge ten times to mix the thawed reagents, and then inspect that all positions are

thawed.

Inspect the reagents in positions 1, 2, and 4 to make sure that they are fully mixed and free of

precipitates.

Gently tap the cartridge on the bench to reduce air bubbles in the reagents.

NOTE

The MiSeq sipper tubes go to the bottom of each reservoir to aspirate the reagents, so it is important

that the reservoirs are free of air bubbles.

Place the reagent cartridge on ice for up to six hours, or set aside at 2°C to 8°C until ready to set up the

run. For best results, proceed directly to loading the sample and setting up the run.

WARNING

This set of reagents contains potentially hazardous chemicals. Personal injury can occur through inhalation,

ingestion, skin contact, and eye contact. Wear protective equipment, including eye protection, gloves, and

laboratory coat appropriate for risk of exposure. Handle used reagents as chemical waste and discard in

accordance with applicable regional, national, and local laws and regulations. For additional environmental,

health, and safety information, see the SDS at support.illumina.com/sds.html.

If necessary for your library type, denature and dilute libraries, and add optional PhiX control. See MiSeq

System Denature and Dilute Libraries Guide (document # 15039740) . If you are performing the VeriSeq

PGS workflow, see the VeriSeq PGS Library Preparation Guide (document # 15052877) .

This step does not apply to all library types . Some Illumina sample preparation methods result in a ready-to-

use normalized concentration of pooled libraries. Refer to the sample preparation guide for the kit used to

prepare sample libraries.

NOTE

If you are using custom primers, prepare primers and set up the sample sheet as described in MiSeq

Custom Primers Guide (document # 15041638) .

When the reagent cartridge is fully thawed and ready for use, load prepared libraries onto the cartridge.

Clean the foil seal covering the reservoir labeled Load Samples with a low-lint lab tissue.

Pierce the foil seal with a clean 1 ml pipette.

Pipette 600 µl of prepared libraries into the reservoir Load Samples. Avoid touching the foil seal.

Document # 15027617 v04 Material # 20024228

For Research Use Only. Not for use in diagnostic procedures.

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