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MiSeq System Guide
Reagents enter the flow cell through the inlet port, pass through the single-lane imaging area, and then exit
the flow cell through the outlet port. Waste exiting the flow cell is delivered to the waste bottle.
Libraries are loaded onto the reagent cartridge before setting up the run, and then automatically transferred
to the flow cell after the run begins.
Figure 7 MiSeq Flow Cell
A Outlet Port
B Imaging Area
C Inlet Port
Flow Cell Cap Color
The cap color of the flow cell container indicates the flow cell type:
Flow Cell
Standard Flow Cell
PGS Flow Cell
Micro Flow Cell
Nano Flow Cell
Reagent Cartridge Overview
The MiSeq reagent cartridge is a single-use consumable consisting of foil-sealed reservoirs prefilled with
clustering and sequencing reagents sufficient for sequencing one flow cell.
Each reservoir on the cartridge is numbered. Sample libraries are loaded onto the cartridge in position 17,
which is labeled Load Samples.
WARNING
This set of reagents contains potentially hazardous chemicals. Personal injury can occur through inhalation,
ingestion, skin contact, and eye contact. Wear protective equipment, including eye protection, gloves, and
laboratory coat appropriate for risk of exposure. Handle used reagents as chemical waste and discard in
accordance with applicable regional, national, and local laws and regulations. For additional environmental,
health, and safety information, see the SDS at support.illumina.com/sds.html.
Document # 15027617 v04 Material # 20024228
For Research Use Only. Not for use in diagnostic procedures.
Flow Cell Cap Color
Clear
Green
Yellow
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