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MiSeq System Guide
dilution.
b Add 50 µl of the 1:25 NaOCl dilution to 950 µl of laboratory-grade water in a MiSeq tube (part # MS-
102-9999).
NOTE
Using the correct concentration of NaOCl is important. Make sure to check the percentage of NaOCl on
the product label. If the concentration is too high, it can make cluster generation fail in subsequent runs.
If 5% NaOCl is not available, make a 1 ml solution of 0.01% NaOCl in laboratory-grade water. Do not use
NaOCl with a maintenance wash or a standby wash
3
Prepare the wash components with fresh wash solution:
a
Add 6 ml wash solution to each reservoir of the wash tray.
b Add 350 ml wash solution to the 500 ml wash bottle.
4
Insert the MiSeq tube containing 0.01% NaOCl wash solution into position 17 of the wash tray until the
neck of the tube is flush with the tray. The tube displaces the Tween 20 and laboratory-grade water
wash solution from position 17.
Figure 22 MiSeq Tube in Position 17 of the Wash Tray
NOTE
Make sure to insert the MiSeq tube with NaOCl into tray position 17 only. Inserting the tube in another
position can make cluster generation fail in subsequent runs, and can damage the fluidic system of the
MiSeq instrument.
5
When the run is complete, select Start Wash. The software automatically raises the sippers in the reagent
chiller.
6
Select Perform optional template line wash on the Post-Run Wash screen.
When using the VeriSeq PGS workflow, the option Perform optional template line wash is pre-selected for
you. The MCS tracks the type of post-run wash performed after each run. If Perform optional template
line wash is not selected for the post-run wash, a message on the Run Review screen reminds you the
next time you start a sequencing run.
7
Open the reagent compartment door and reagent chiller door, and slide the used reagent cartridge from
the chiller.
8
Slide the wash tray into the reagent chiller until it stops, and then close the reagent chiller door.
9
Raise the sipper handle in front of the PR2 bottle and waste bottle until it locks into place.
10 Remove the PR2 bottle and replace it with the wash bottle.
NOTE
Discard the PR2 bottle after each run. Do not reuse any remaining PR2.
Document # 15027617 v04 Material # 20024228
For Research Use Only. Not for use in diagnostic procedures.
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