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MiSeq System Guide
Raise Reagent Cartridge Sippers Manually
The reagent cartridge sippers might not raise automatically if a run was interrupted unexpectedly, or if an
error occurred during the run. To remove the reagent cartridge, raise the reagent cartridge sippers manually.
1
On the Home screen, select Perform Wash.
2
Select Raise Sippers.
3
Remove the reagent cartridge.
Resolve Run Setup Errors
If any checks in the pre-run check fail, a red icon
screen that describes the error and how to correct it.
Error
Free Disk Space
Network Connection Active
Primary Analysis Ready
Resolve RFID Read Failure
If the system cannot read the RFID of a consumable, you can obtain a temporary bypass code from the
Illumina website. A temporary bypass code expires in seven days.
1
Always retry the RFID read before proceeding. If the RFID fails a second time, select Enter Code.
2
From a computer with internet access, go to
top of the page.
3
Log in to your MyIllumina account.
Your name replaces the Sign In button on the toolbar.
4
Hover over your name and select Account. In the My Tools column, click MiSeq Self-Service.
5
On the MiSeq Self-Service page, enter the MiSeq serial number.
6
From the Type of Override Code drop-down list, select RFID Override.
7
To generate the code, select Get Code.
8
Return to the MCS interface and select Enter Code.
9
Enter the temporary bypass code using the on-screen keyboard, and then select Next.
10 Enter the barcode number of the flow cell, PR2 bottle, or reagent cartridge.
Document # 15027617 v04Material # 20024228
For Research Use Only. Not for use in diagnostic procedures.
appears next to the item. A message appears on the
Action
If disk space is low, a message appears indicating how much disk space is required. Use
the Manage Files feature to clear the required space from the instrument computer.
Make sure that the network cable is plugged into the instrument.
If the network connection is not restored, select Reboot on the Manage Instrument screen
to reboot the software.
If the connection is still not restored, select Shut Down on the Manage Instrument screen,
and then turn off the instrument using the power switch. Wait at least 60 seconds, and then
turn on the instrument and start the software.
Primary analysis from the previous run is not complete. The default time to allow primary
analysis to complete is 1 hour, and a countdown appears on the screen. The options are to
wait 1 hour or select Terminate Analysis. Secondary analysis stops for any incomplete
cycles.
my.illumina.com
and select Sign In from the toolbar on the
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