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illumina Infinium LiHa Operating Manual

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Operating Manual
Infinium LiHa and Infinium RoMa
Infinium LiHa and Infinium RoMa Operating Manual - Document # 1000000110155 v00

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Summary of Contents for illumina Infinium LiHa

  • Page 1 Operating Manual Infinium LiHa and Infinium RoMa Infinium LiHa and Infinium RoMa Operating Manual - Document # 1000000110155 v00...
  • Page 2 © 2020, Illumina, Inc. All rights reserved. All trademarks are the property of Illumina, Inc. or their respective owners.

  • Page 3: Manufacturer

    0 - Preface Manufacturer 0 Preface For Your Safety Before performing any work on or with the Infinium LiHa and Infinium RoMa, first read the Operating Manual - Document # 1000000110155 v00 carefully, in particular chapter 2 “Safety”. 0.1 Manufacturer...

  • Page 4: Ce Conformity

    CE Conformity 0.3 CE Conformity Declaration of The Infinium LiHa and Infinium RoMa is designed and built in compliance with the basic safety and health requirements of applicable EC Directives. With the Conformity declaration of conformity the manufacturer declares conformity with the provisions of the Directives.

  • Page 5: Fcc Rules

    Operation of this equipment in a residential area is likely to cause harmful interference in which case users will be required to correct the interference at their own expense. Infinium LiHa and Infinium RoMa Operating Manual - Document # 1000000110155 v00 0 - 3...
  • Page 6 0 - Preface FCC Rules 0 - 4 Infinium LiHa and Infinium RoMa Operating Manual - Document # 1000000110155 v00...

  • Page 7: Table Of Contents

    Decontamination Declaration ........2-13 Infinium LiHa and Infinium RoMa Operating Manual - Document # 1000000110155 v00 1 - 1...
  • Page 8 Introduction..........3.1.1 Infinium LiHa and Infinium RoMa Overview ..... 3.1.2 Product Identification and Labeling .
  • Page 9 Storage........... . Infinium LiHa and Infinium RoMa Operating Manual - Document # 1000000110155 v00...
  • Page 10 Documentation ..........11-1 11.3 Infinium LiHa and Infinium RoMa Basic Accessories Kit ....11-1 11.4 Carriers, Racks, Troughs .

  • Page 11: About This Manual

    Target Group This manual is intended for everyone who wants to learn about the safe operation of the Infinium LiHa and Infinium RoMa and how to maintain its perfect working condition. In particular, laboratory personnel and operators are addressed. Laboratory personnel operating Infinium LiHa and Infinium RoMa instruments require also thorough knowledge of applications, instrument functions and software programs as well as all applicable safety rules and regulations.

  • Page 12: Reference Documents

    Instruments Any individual or separate operating manuals for optional equipment according to your order configuration can be applicable. For information about the liquids to use with the Infinium LiHa and Infinium RoMa, refer to section 3.5 “Chemical Resistance”,  3-17.

  • Page 13: Abbreviations

    Pressure based liquid level detection Pressure monitored pipetting Polyoxymethylene Polypropylene PosID Positive identification option, barcode scanner Polystyrene PTFE Polytetrafluoroethylene Polyvinylchloride PVDF Polyvinylidenefluoride RoMa Robotic manipulator arm Radio frequency Infinium LiHa and Infinium RoMa Operating Manual - Document # 1000000110155 v00 1 - 3...
  • Page 14 1 - About This Manual Abbreviations Sensored pump option Universal serial bus Uninterruptable power supply World Health Organization 1 - 4 Infinium LiHa and Infinium RoMa Operating Manual - Document # 1000000110155 v00...

  • Page 15: Safety

    2 - Safety Safety Message Conventions 2 Safety This chapter describes the safety concept of Infinium LiHa and Infinium RoMa, provides general rules of correct behavior, and warnings concerning hazards associated with the use of the Infinium LiHa and Infinium RoMa.
  • Page 16 2 - Safety Safety Message Conventions Hot surface hazard Wear protective gloves Read this Disturbance of functions by electromagnetic RF waves. Do not use a cellular phone. 2 - 2 Infinium LiHa and Infinium RoMa Operating Manual - Document # 1000000110155 v00...

  • Page 17: General Safety Information

    Nevertheless, risks to users, property and the environment can arise if the Infinium LiHa and Infinium RoMa is used without due care and attention. The safety of all users and personnel depends on the strict observation of these safety instructions and awareness of the safety-related warnings provided in this manual.
  • Page 18 Infinium LiHa and Infinium RoMa. The same applies to waste disposal. – Always be aware of possible hazards associated with these substances. 2 - 4 Infinium LiHa and Infinium RoMa Operating Manual - Document # 1000000110155 v00...

  • Page 19: Operating Company

    For more information go to www.P65Warnings.ca.gov/product. 2.3 Operating Company The operating company must ensure that the Infinium LiHa and Infinium RoMa, in particular the safety features, function properly and that all the personnel in contact with the instrument are adequately trained.

  • Page 20: User Qualification

    User Qualification 2.4 User Qualification The laboratory personnel must be fully qualified and trained to operate the Infinium LiHa and Infinium RoMa. The work described in this Operating Manual must only be performed by authorized personnel with the qualifications prescribed below.

  • Page 21: Safety Elements

    Door Locks During operation the front safety panel is locked by means of two door locks. The safety concept of the Infinium LiHa and Infinium RoMa assumes that the front safety panel is always closed when the instrument is running.
  • Page 22 Side safety panel Cutout for continuous loading General Removal of The protective and safety devices installed on the Infinium LiHa and Infinium RoMa must not be removed or disabled during operation. Safety Elements In the event such elements were removed, e.g. for maintenance work, operation may only be resumed when all protective and safety devices have been completely installed and checked.

  • Page 23: Product Safety Signs

    2.6 Product Safety Signs Where are Infinium LiHa and Infinium RoMa Instrument Safety Notices The figure shows the safety notices that are attached to the Infinium LiHa and Attached? Infinium RoMa instrument. It also shows their locations: Fig. 2-2 Safety notices attached to the product The following table explains the significance of the notices: Tab.

  • Page 24: Laser Radiation

    Class 2 Laser Product pursuant to IEC 60825-1:2007: "Complies with 21 CFR 1040.10 except for deviations pursuant to Laser Notice No. 50, dated June 24, 2007" 2 - 10 Infinium LiHa and Infinium RoMa Operating Manual - Document # 1000000110155 v00...
  • Page 25 On barcode scanner the scanner head assembly by hand which could head, see Fig. 2-5,  2-11 damage motor and head assembly. a) According to IEC/EN 60825-1 Infinium LiHa and Infinium RoMa Operating Manual - Document # 1000000110155 v00 2 - 11...
  • Page 26 The only possible health hazard is in exposing the eyes to the laser beam.  Do not stare into the beam. 2 - 12 Infinium LiHa and Infinium RoMa Operating Manual - Document # 1000000110155 v00...

  • Page 27: Decontamination Declaration

    Repair Order) in case the owner of the instrument has no template for such a declaration at hand. Contact the Illumina helpdesk for further information. Note: Illumina reserves the right to refuse any instrument or a part of it, or will charge an extra fee, if the decontamination is not declared sufficiently.
  • Page 28 2 - Safety Decontamination Declaration 2 - 14 Infinium LiHa and Infinium RoMa Operating Manual - Document # 1000000110155 v00...

  • Page 29: Technical Data 3.1 Introduction

    3 - Technical Data Introduction 3 Technical Data Purpose of This This chapter introduces the reader to the Infinium LiHa and Infinium RoMa and its main components. It contains technical data, requirements and performance data. Chapter 3.1 Introduction What is The Infinium LiHa and Infinium RoMa is a precision instrument designed for automating specific steps in the Illumina Infinium Array Workflow.

  • Page 30: Product Identification And Labeling

    The identification data is also printed on the serial number label (B). This label can be viewed from the front side of the instrument. It is attached below the diluters. Label 3 - 2 Infinium LiHa and Infinium RoMa Operating Manual - Document # 1000000110155 v00...

  • Page 31: Technical Data

    The figure shows the dimensions of the instrument: Overall Dimensions 780 mm (30.7 1224 mm (48.2 *maximum height for objects under the x rail Fig. 3-2 Instrument overall dimensions Infinium LiHa and Infinium RoMa Operating Manual - Document # 1000000110155 v00 3 - 3...

  • Page 32: Supplies

    (100 - 120 VAC) must not exceed 1000 VA in order to keep the input current below the fused values. 3 - 4 Infinium LiHa and Infinium RoMa Operating Manual - Document # 1000000110155 v00...

  • Page 33: Status Lamp

    When the status lamp light is red, an acoustic alarm sounds (status lamp functions: Refer to 6.2 “Operating Modes”,  6-3). The illuminated area is 540 x 18 mm (21.26 x 0.7 in.). Infinium LiHa and Infinium RoMa Operating Manual - Document # 1000000110155 v00 3 - 5...

  • Page 34: Environmental Conditions

    Conditions Transport temperature -20°C to 60°C (-4°F to 140°F) for maximum 24 hours Transport humidity 20% to 80% relative (non condensing) for maximum 24 hours 3 - 6 Infinium LiHa and Infinium RoMa Operating Manual - Document # 1000000110155 v00...

  • Page 35: Emissions

    The instrument is connected via USB to the control PC. Tab. 3-6 USB Interface Specification (Instrument) Interface Voltage Current USB 2.0 4.4 V to 5.25. V max. 500 mA Infinium LiHa and Infinium RoMa Operating Manual - Document # 1000000110155 v00 3 - 7...

  • Page 36: Requirements

    -the art by the kit manufacturer or operator of the system. For sample preparation refer to cross references above. 3 - 8 Infinium LiHa and Infinium RoMa Operating Manual - Document # 1000000110155 v00...

  • Page 37: System Modules

     Y-axis 8-tip LiHa 373 mm (14.7 in.) Z-axis 210 mm (8.27 in.) a) at 9 mm spacing b) each channel individually, no tips installed Infinium LiHa and Infinium RoMa Operating Manual - Document # 1000000110155 v00 3 - 9...
  • Page 38 The equidistant movement of sampling tips in Y direction is: Movement  from 9 mm ± 0.4 mm  to 38 mm ± 1 mm 3 - 10 Infinium LiHa and Infinium RoMa Operating Manual - Document # 1000000110155 v00...
  • Page 39 PCTFE (Kel-F) Syringes Borosilicate glass Syringes, seals PTFE Tips Stainless steel, PTFE Liquid containers HD-PE a) Coating Also refer to section 3.5 “Chemical Resistance”,  3-17. Infinium LiHa and Infinium RoMa Operating Manual - Document # 1000000110155 v00 3 - 11...

  • Page 40: Robotic Manipulator Arm Standard (Roma)

    270° (left or right oriented) ATTENTION Improper transport of labware (microplates, etc.) Use only labware that is rigid enough not to be deformed by the gripper force. 3 - 12 Infinium LiHa and Infinium RoMa Operating Manual - Document # 1000000110155 v00...

  • Page 41: Positive Identification (Posid)

    Container ID barcode, 100 ml trough on carrier 100 mm/s 99.8% a) Barcode scanner b) “No Tube” sensor, glass or plastic tube, filled or empty, with or without barcode Infinium LiHa and Infinium RoMa Operating Manual - Document # 1000000110155 v00 3 - 13...
  • Page 42 Also used for standard carrier ID barcodes b) The application SW may restrict the use of barcode types. See section “Permissible Barcode Types” below. 3 - 14 Infinium LiHa and Infinium RoMa Operating Manual - Document # 1000000110155 v00...
  • Page 43 To avoid misreadings, the quality of the barcode labels must be graded A, B or C according to ANSI X3.182 and DIN EN 1635. Illumina recommends using grade A for best reading performance. A quality system in the production of barcode labels must be employed to ensure the conformity to the quality grades mentioned above.
  • Page 44 Note: Depending on the application software, not all barcode types readable by the PosID may be supported. Refer to your Application Software Manual to find out the permissible barcode types. 3 - 16 Infinium LiHa and Infinium RoMa Operating Manual - Document # 1000000110155 v00...

  • Page 45: Chemical Resistance

    Ethanol 96 % Formaldehyde 40 % Sulfuric acid 40 % Sulfuric acid 96 % Isopropanol Diluted bleach, NaOCl Methanol Methylene chlo- ride Sodium hydroxide Infinium LiHa and Infinium RoMa Operating Manual - Document # 1000000110155 v00 3 - 17...
  • Page 46 32 % Trichloroacetic acid 40 % a) Kel-F Legend: resistant partly resistant, use is possible with frequent replacements not resistant, unsuitable for use not determined 3 - 18 Infinium LiHa and Infinium RoMa Operating Manual - Document # 1000000110155 v00...

  • Page 47: Introduction

    4 - Description of Function Introduction 4 Description of Function Purpose of This This chapter explains the basic principle of the Infinium LiHa and Infinium RoMa, shows how it is structured and gives a functional description of the assemblies. Chapter 4.1 Introduction...

  • Page 48: Structure

    4 - Description of Function Structure 4.2 Structure 4.2.1 Mechanical Structure The figure shows the main parts of the Infinium LiHa and Infinium RoMa: Fig. 4-1 Infinium LiHa and Infinium RoMa instrument overview Housing Worktable with positioning pins Diluters with syringes...

  • Page 49: The Infinium Liha And Infinium Roma Worktable

    Waste tubing Syringe In case of a second liquid handling arm, each LiHa is equipped with its own parts, i.e. the two liquid systems are independent. Infinium LiHa and Infinium RoMa Operating Manual - Document # 1000000110155 v00 4 - 3...

  • Page 50: Function

    The liquid handling arm is part of the liquid system and is used for pipetting tasks. Fig. 4-3 The liquid handling arm, LiHa Syringes Tips Liquid handling arm 4 - 4 Infinium LiHa and Infinium RoMa Operating Manual - Document # 1000000110155 v00...
  • Page 51 The LiHa arm holds electronics to detect the level of liquids in plate wells, tubes and liquid containers. For details refer to section “”,  4-14. Detection Infinium LiHa and Infinium RoMa Operating Manual - Document # 1000000110155 v00 4 - 5...

  • Page 52: Robotic Manipulator Arm Standard (Roma Standard)

    Robotic manipulator arm RoMa Axis for gripper movements Axis from front to back of worktable Rotational axis Vertical axis above worktable Axis from left to right of worktable 4 - 6 Infinium LiHa and Infinium RoMa Operating Manual - Document # 1000000110155 v00...

  • Page 53: Barcode Scanner On Roma

    Illumina or Clarity LIMS, or their own custom LIMS system. The Illumina 1D barcode scanner kit is installed on the RoMa arm for all such customers. It is mounted as per ILMN FSB 506 on the bottom of the RoMa arm using special mounting hardware and an adjustable dual bracket, then connected electrically via its cable to the existing RoMa arm cable.

  • Page 54: Safety Elements

    The front safety panel is secured in closed position with the door locks. Panel According to the size of the Infinium LiHa and Infinium RoMa and the kind of front safety panel, one or two gas springs facilitate the opening of the panel.
  • Page 55 (B), which is mounted to the safety panel. A switch in the locking device monitors if the safety panel is open or closed. Infinium LiHa and Infinium RoMa Operating Manual - Document # 1000000110155 v00 4 - 9...

  • Page 56: Positive Identification (Posid)

    This improves identification security for the containers. 4 - 10 Infinium LiHa and Infinium RoMa Operating Manual - Document # 1000000110155 v00...
  • Page 57 The figure shows how vertical barcodes (e.g. on tubes or reagent troughs) are scanned. Fig. 4-10 Barcode scanner position for scanning vertical barcodes PosID body Container barcode label Barcode scanner Carrier Infinium LiHa and Infinium RoMa Operating Manual - Document # 1000000110155 v00 4 - 11...
  • Page 58 Fig. 4-12 Detectable situations in a tube rack Tubes with readable barcode Tubes without barcode (or incorrect positioned barcode) No tubes present Carrier ID barcode 4 - 12 Infinium LiHa and Infinium RoMa Operating Manual - Document # 1000000110155 v00...
  • Page 59 ID with the database, the software is able to identify the carrier’s properties. Barcode Labels For detailed information on barcode types and proper positioning of barcode labels on carriers and containers refer to cross references above. Infinium LiHa and Infinium RoMa Operating Manual - Document # 1000000110155 v00 4 - 13...

  • Page 60: Liquid System

    Wash station Distributor 1:4 (1:2 for 2-tip instrument) Waste tubing Interconnecting tubing Waste container 3-way valve Syringe Note: The arrows indicate the direction of flow. 4 - 14 Infinium LiHa and Infinium RoMa Operating Manual - Document # 1000000110155 v00...

  • Page 61: Tubing Systems

    Standard tubing system composed of PVC/silicone/PP/ Pipetting With all tubing systems, the pipetting tubing is made of FEP, which is resistant against a wide range of liquids. Tubing Infinium LiHa and Infinium RoMa Operating Manual - Document # 1000000110155 v00 4 - 15...
  • Page 62 4 - Description of Function Liquid System 4 - 16 Infinium LiHa and Infinium RoMa Operating Manual - Document # 1000000110155 v00...

  • Page 63: Putting Into Operation

    5 - Putting into Operation Installation 5 Putting into Operation Purpose of This This chapter describes how the Infinium LiHa and Infinium RoMa is installed and gives instructions on initial operation. Chapter 5.1 Installation 5.1.1 Initial Installation of the Instrument The initial installation of the instrument may be done by a qualified Illumina service person only.
  • Page 64 5 - Putting into Operation Startup 5 - 2 Infinium LiHa and Infinium RoMa Operating Manual - Document # 1000000110155 v00...

  • Page 65: Operation

    6 Operation Purpose of This This chapter explains the operating elements and possible operating modes. It gives instructions on how to operate the Infinium LiHa and Infinium RoMa properly Chapter and safely. For the options used by your configuration, refer to the separate operating manuals.
  • Page 66 Display functions and controls are available in the software packages and user interfaces on the PC. Depending on your application, refer to the relevant separate documentation. 6 - 2 Infinium LiHa and Infinium RoMa Operating Manual - Document # 1000000110155 v00...

  • Page 67: Operating Modes

    6 - Operation Operating Modes 6.2 Operating Modes Possible The Infinium LiHa and Infinium RoMa can be run in two different operating modes: Operating  Routine operation mode (operator) Modes – This is the normal operating mode, in which the application is run.
  • Page 68 To ensure a proper liquid flow, make sure that the tubings are not twisted or inhibited from free flow. ATTENTION Instruments are intended for indoor operation with controlled temperatures.It is important to maintain constant temperature plus air gaps. 6 - 4 Infinium LiHa and Infinium RoMa Operating Manual - Document # 1000000110155 v00...
  • Page 69 Using liquids with undissolved particles could lead to clogged tips and thus result in liquid not being dispensed.  Clogging can also result if the tips have not been thoroughly washed. Infinium LiHa and Infinium RoMa Operating Manual - Document # 1000000110155 v00 6 - 5...
  • Page 70 6 - Operation Operating in Routine Operation Mode Applications For all applications of the Illumina instrument, the user must ensure that the requirements of each protocol are carefully observed. Attention must be given to:  Sample/reagent volumes and concentrations ...

  • Page 71: Enclosed Work Area

    Automatically moving parts. If the safety panels are not in place, injuries (crushing, piercing) are possible. Before starting the Infinium LiHa and Infinium RoMa, make sure that the safety panel is closed. Never operate the instrument with the panel open.
  • Page 72 6 - Operation Operating in Routine Operation Mode To switch the Infinium LiHa and Infinium RoMa on, proceed as follows: Press the power ON/OFF switch for 0.5 seconds to switch the instrument on. Wait until the status light in the power ON/OFF switch is lit.

  • Page 73: Instrument Preparation And Checks

    Improper positioning of objects on the worktable may lead to disturbances or errors in the process, e.g. misinterpretation of barcodes. Do not use free space on the worktable to deposit any objects Infinium LiHa and Infinium RoMa Operating Manual - Document # 1000000110155 v00 6 - 9...
  • Page 74 RoMa are dropped after a restart.  Visually inspect the arm devices whether they still hold any objects between their grippers.  Remove such objects before starting up the instrument. 6 - 10 Infinium LiHa and Infinium RoMa Operating Manual - Document # 1000000110155 v00...
  • Page 75 Make sure that the correct rack is used for the carrier. If a carrier is damaged, replace it immediately. Carrier ID Each carrier ID must be unique. Infinium LiHa and Infinium RoMa Operating Manual - Document # 1000000110155 v00 6 - 11...
  • Page 76 Microplates must be positioned correctly on the carrier, seating well in their holder. Make sure that the microplate does not rest on the holder rim in a slanting position. 6 - 12 Infinium LiHa and Infinium RoMa Operating Manual - Document # 1000000110155 v00...
  • Page 77 Make sure that all tubes are positioned correctly in the carrier and touch the rack bottom, otherwise liquid level detection and clot detection might not work properly. Infinium LiHa and Infinium RoMa Operating Manual - Document # 1000000110155 v00 6 - 13...
  • Page 78 Note: The filling level of tubes, troughs and containers must not exceed 80% to avoid spillage during PosID reading. Tab. 6-2 Minimum inner diameter for primary sample tubes Tip type Tube diameter, inside Fixed tips 7 mm 6 - 14 Infinium LiHa and Infinium RoMa Operating Manual - Document # 1000000110155 v00...
  • Page 79  Separate the bypass tubing and the aspiration tube in such a way that no air bubbles can be aspirated. Infinium LiHa and Infinium RoMa Operating Manual - Document # 1000000110155 v00 6 - 15...
  • Page 80  The waste tubing must not sag (back pressure).  The lower end of the waste tubing must not be in the liquid (back pressure). 6 - 16 Infinium LiHa and Infinium RoMa Operating Manual - Document # 1000000110155 v00...

  • Page 81: Checks And Terminating Tasks

    Press the power ON/OFF switch and keep it pressed for at least 2 seconds. ATTENTION Wait until the status light in the power switch is off (for approx. 10 sec.) before switching the instrument on again. Infinium LiHa and Infinium RoMa Operating Manual - Document # 1000000110155 v00 6 - 17...

  • Page 82: When A Crash Occurred

    For detailed descriptions of the maintenance tasks, refer to 7 “Preventive Maintenance and Repairs”, in this Operating Manual - Document # 1000000110155 v00. 6 - 18 Infinium LiHa and Infinium RoMa Operating Manual - Document # 1000000110155 v00...

  • Page 83: Preventive Maintenance And Repairs

    Principle Only operate the Infinium LiHa and Infinium RoMa when it is in good working condition. Strictly observe the maintenance instructions as set out in this manual. To achieve specified performance and reliability of the instrument, regularly carry out the maintenance and cleaning tasks.
  • Page 84 Please contact www.deconlabs.com the manufacturer Liqui-Nox Weak detergent Alconox Please contact www.alconox.com the manufacturer a) These are identical products; are hereafter called Decon / Contrad 7 - 2 Infinium LiHa and Infinium RoMa Operating Manual - Document # 1000000110155 v00...
  • Page 85 PosID scanner head Alcohol laser beam output win- Arm guide, arm guide Do not use any agent roller of arms Z-rod Do not use any agent Infinium LiHa and Infinium RoMa Operating Manual - Document # 1000000110155 v00 7 - 3...
  • Page 86 Cleaning Cleaning Tissue Use a lint-free tissue together with the appropriate cleaning agent. 7 - 4 Infinium LiHa and Infinium RoMa Operating Manual - Document # 1000000110155 v00...

  • Page 87: Maintenance Schedule

    (depending on configuration) maintenance carried out by a Illumina authorized field service engineer (FSE) is recommended. Maintenance Note: In order to be able to track all maintenance performed on the Infinium LiHa Record and Infinium RoMa over the whole lifetime, the periodic maintenance must be recorded as follows: •...
  • Page 88 Tab. 7-6 Daily maintenance during the day Instrument/ Component Maintenance Task Reference Liquid system Flush prior to each application See section 7.3.1.2 “Flushing the Liquid System”,  7-12 7 - 6 Infinium LiHa and Infinium RoMa Operating Manual - Document # 1000000110155 v00...
  • Page 89  7-10 If liquids other than water are See section 7.3.1.2 “Flushing the used as system liquid, flush with Liquid System”,  7-12 deionized water Infinium LiHa and Infinium RoMa Operating Manual - Document # 1000000110155 v00 7 - 7...

  • Page 90: Maintenance Table: Weekly Maintenance

    Call Illumina customer support especially Y-belt, for wear and to perform the task. replace faulty parts. Check parts for abrasion; wipe wear debris off, if necessary. 7 - 8 Infinium LiHa and Infinium RoMa Operating Manual - Document # 1000000110155 v00...
  • Page 91 Please contact your local service organization, for further information on maintenance tasks and schedule of your system. Infinium LiHa and Infinium RoMa Operating Manual - Document # 1000000110155 v00 7 - 9...

  • Page 92: Maintenance Tasks

    Leakages in the liquid system can also be caused by an empty liquid system or by aggressive liquids. When using aggressive liquids, take into account the chemical resistance of the tubing material. Refer to cross references above. 7 - 10 Infinium LiHa and Infinium RoMa Operating Manual - Document # 1000000110155 v00...
  • Page 93 ATTENTION A leaking liquid system causes pipetting inaccuracy and cross-contamination.  Never operate the Infinium LiHa and Infinium RoMa if the liquid system is leaking. Infinium LiHa and Infinium RoMa Operating Manual - Document # 1000000110155 v00 7 - 11...
  • Page 94 ATTENTION Air bubbles in the liquid system cause pipetting inaccuracy.  Never operate the Infinium LiHa and Infinium RoMa with air bubbles in the liquid system. 7 - 12 Infinium LiHa and Infinium RoMa Operating Manual - Document # 1000000110155 v00...
  • Page 95 Place the tubing in a bottle with distilled or de-ionized water and flush the liquid system twice. Refer to cross references above. Flush the liquid system eight times with system liquid. Refer to cross references above. Infinium LiHa and Infinium RoMa Operating Manual - Document # 1000000110155 v00 7 - 13...

  • Page 96: Syringe

    Fig. 7-2 Syringe and valve Syringe screw Plunger lock screw If leakages still occur, replace the syringe or the syringe cap. Refer to cross references above. 7 - 14 Infinium LiHa and Infinium RoMa Operating Manual - Document # 1000000110155 v00...

  • Page 97: Fixed Tips Of Liha

    If a tip is to be reinstalled, do not remove the lock nut from the tip.  Always hold the tip at its upper end, avoiding contact with the coated surface whenever possible. Infinium LiHa and Infinium RoMa Operating Manual - Document # 1000000110155 v00 7 - 15...
  • Page 98 Refer to cross references above. Reinstall the wash station on the worktable. Make sure that the wash station is pushed all the way back to the stop during installation. 7 - 16 Infinium LiHa and Infinium RoMa Operating Manual - Document # 1000000110155 v00...

  • Page 99: Worktable

    Contamination through waste liquid, if the containers are installed wrongly. Make sure not to mix up the system liquid container and the waste container.  Infinium LiHa and Infinium RoMa Operating Manual - Document # 1000000110155 v00 7 - 17...

  • Page 100: Carriers And Racks

    Carriers and Racks Perform the following procedure to clean the carriers and racks. Remove all carriers and racks from the Infinium LiHa and Infinium RoMa worktable. The wash station can be cleaned on the worktable. Before cleaning, remove the barcode labels from the carriers, if possible.

  • Page 101: Positive Identification (Posid)

     Do not attempt to rotate the barcode scanner manually.  Use the initialization routine to run the barcode scanner into maintenance position. Infinium LiHa and Infinium RoMa Operating Manual - Document # 1000000110155 v00 7 - 19...
  • Page 102 Tube” sensor (B). Moisten a lint-free tissue with alcohol and clean the front surface of the “No Tube” sensor. Fig. 7-5 PosID “No Tube” sensor 7 - 20 Infinium LiHa and Infinium RoMa Operating Manual - Document # 1000000110155 v00...

  • Page 103: Arm Guide

    Note: Do not use alcohol or solvents to clean the arm guide. Do not use grease on the arm rails. Fig. 7-6 Arm guide and roller Arm guide Arm rail Arm guide roller Infinium LiHa and Infinium RoMa Operating Manual - Document # 1000000110155 v00 7 - 21...

  • Page 104: Precision And Function Tests

    Nucleic Acid solution. Residues Appropriate commercially available agents (e.g. DNAzap) are used to keep the pipetting area (worktable, carriers etc.) free of interfering nucleic acids. 7 - 22 Infinium LiHa and Infinium RoMa Operating Manual - Document # 1000000110155 v00...

  • Page 105: Troubleshooting

    Which Errors The troubleshooting table below lists possible malfunctions and errors of the Infinium LiHa and Infinium RoMa. The operator is enabled to correct some of can the those problems or errors by him/herself. For this, appropriate corrective measures Operator are listed in the column “Corrective measures”.
  • Page 106 Put microplate on carrier Cannot pick up microplate Set gripper position Clean grippers Unusual noise during arm Worn or damaged parts Contact your local service organiza- movement tion 8 - 2 Infinium LiHa and Infinium RoMa Operating Manual - Document # 1000000110155 v00...
  • Page 107 Algae block the wash sta- Clean the wash station tion Waste tubing kinked Check tubing for kinks Infinium LiHa and Infinium RoMa Operating Manual - Document # 1000000110155 v00 8 - 3...
  • Page 108 8 - Troubleshooting Troubleshooting Table 8 - 4 Infinium LiHa and Infinium RoMa Operating Manual - Document # 1000000110155 v00...

  • Page 109: Shutdown, Transport And Storage 9.1 Shutdown

    9 - Shutdown, Transport and Storage Shutdown 9 Shutdown, Transport and Storage Purpose of This This chapter instructs how to shut down the Infinium LiHa and Infinium RoMa, how to pack it for storage or transport, and specifies the storage and shipping Chapter conditions.
  • Page 110 Unplug the power cord from the power supply at the rear of the instrument. Fig. 9-3 RS232 interface on Optibo PCB RS232 interface connector USB connector Disconnect the instrument from the PC. 9 - 2 Infinium LiHa and Infinium RoMa Operating Manua -l Document # 1000000110155 v00...

  • Page 111: Reporting

    Clean and, if necessary, decontaminate the entire instrument. 9.1.2 Reporting Fill out a copy of the decontamination form and place it with the instrument. Record the shut down in the appropriate CRM. Infinium LiHa and Infinium RoMa Operating Manua -l Document # 1000000110155 v00 9 - 3...

  • Page 112: Transport

    Illumina person only Transport The transport of the instrument may be done under direction of a qualified Illumina service person only. Due to the heavy weight, trained relocation staff is needed to lift the instrument.

  • Page 113: Storage

    Protect the instrument against dust and debris with a cover. For long term storage, pack the instrument in its original packing. Store all manuals and the “Maintenance and Service Logbook” with the instrument. Infinium LiHa and Infinium RoMa Operating Manua -l Document # 1000000110155 v00 9 - 5...
  • Page 114 9 - Shutdown, Transport and Storage Storage 9 - 6 Infinium LiHa and Infinium RoMa Operating Manua -l Document # 1000000110155 v00...

  • Page 115: Disposal

    Product In accordance with the requirements specified in SJ/T11364-2014, all electronic and electrical Illumina products sold in the People’s Republic of China are labeled Marking with a marking for the restriction of the use of hazardous substances.
  • Page 116 10 - 2 Infinium LiHa and Infinium RoMa Operating Manual - Document # 1000000110155 v00...

  • Page 117: Spare Parts And Accessories 11.1 Software

    Infinium LiHa and RoMa Accessory Kit Not for sale: KIT ACCESSORY INFINIUM ILLUMINA SCREWDRIVER 4.5/1.5*90 MM BRASS PLT.NI SCREWDRIVER SIZE 2 WORKTABLE MOUNT- SET WRENCH HEXAGON WRENCH HEXAGON 0.71 Infinium LiHa and Infinium RoMa Operating Manual - Document # 1000000110155 v00 11 - 1...

  • Page 118: Carriers, Racks, Troughs

    11-2 landscape orientation ASSY ROMA ILLUMINA 150 mm (5.9 in.) a) Number or grid positions the carrier occupies Fig. 11-1 Carrier for 3 microplates landscape 11 - 2 Infinium LiHa and Infinium RoMa Operating Manual - Document # 1000000110155 v00...

  • Page 119: Carriers For Tubes

    TUBE 16MM 6 PCE. 25 mm  11-3 (0.98 in.) a) Number or grid positions the carrier occupies Fig. 11-2 Carrier for tubes (example for 16 tubes) Infinium LiHa and Infinium RoMa Operating Manual - Document # 1000000110155 v00 11 - 3...

  • Page 120: Wash Stations

    1 waste position at center CAR. (0.98 in.) 8 wash positions deep at front a) Number or grid positions the carrier occupies Fig. 11-3 Wash/waste station, standard 11 - 4 Infinium LiHa and Infinium RoMa Operating Manual - Document # 1000000110155 v00...

  • Page 121: Tips And Accessories

    11 - Spare Parts and Accessories Tips and Accessories 11.5 Tips and Accessories Contact your local service representative for information on other accessories. Infinium LiHa and Infinium RoMa Operating Manual - Document # 1000000110155 v00 11 - 5...
  • Page 122 11 - Spare Parts and Accessories Tips and Accessories 11 - 6 Infinium LiHa and Infinium RoMa Operating Manual - Document # 1000000110155 v00...

  • Page 123: Customer Support 12.1 Contacts

    +33 805102193 +33 170770446 Germany +49 8001014940 +49 8938035677 Hong Kong, China 800960230 Ireland +353 1800936608 +353 016950506 Italy +39 800985513 +39 236003759 Japan 0800.111.5011 Infinium LiHa and Infinium RoMa Operating Manual - Document # 1000000110155 v00 12 - 1...
  • Page 124 +34 911899417 +34 800300143 Sweden +46 850619671 +46 200883979 Switzerland +41 565800000 +41 800200442 Taiwan, China 00806651752 United Kingdom +44 8000126019 +44 2073057197 Other countries +44.1799.534000 12 - 2 Infinium LiHa and Infinium RoMa Operating Manual - Document # 1000000110155 v00...

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