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MiSeq System Guide
Consumable
Barcode Number Location
Flow Cell
Above the barcode on the flow cell container label.
Flow cell barcode numbers begin with an A (standard), G (micro), or D (nano). Example: A0E61
PR2 Bottle
Below the barcode on the PR2 bottle label.
Example: MS0011881-PR2
Reagent Cartridge
Below the barcode on the reagent cartridge label.
Example: MS0010744-300
11 If you are entering a bypass code for the reagent cartridge, enter the version number of the kit. Select
Enter Reagent Kit Barcode to enter the reagent cartridge barcode number and kit version number
manually.
CAUTION
Entering the incorrect reagent kit version can negatively affect sequencing data.
12 Select Enter.
Perform a Volume Test
An obstruction in the fluidics lines can cause poor reagent delivery and affect sequencing results. If an
obstruction in the fluidics lines is suspected, perform a volume test.
A volume test checks the health of the fluidics system by estimating the volume between bubbles as they
pass by the sensors. To perform a volume test, the wash tray and wash bottle must be loaded with
laboratory-grade water and a used flow cell must be in place. Follow the onscreen prompts to perform the
test.
1
Make sure that a used flow cell is loaded on the instrument.
2
From the Home screen, select Manage Instrument.
3
Select System Check.
4
Select Conduct Volume Test, and then select Next.
5
Fill each reservoir of the wash tray with 6 ml laboratory-grade water.
6
Fill the 500 ml wash bottle with 350 ml laboratory-grade water.
7
Load the wash tray and wash bottle onto the instrument.
a
Open the reagent compartment door and reagent chiller door, and slide the wash tray into the
reagent chiller until it stops. Close the reagent chiller door.
b Remove the waste bottle and discard the contents appropriately. Return the waste bottle to the
reagent compartment.
c
Slowly lower the sipper handle, making sure that the sippers lower into the wash bottle and waste
bottle.
8
Select Next.
When the volume test is complete, the results appear on the screen.
If the test did not pass, perform a maintenance wash. See
9
When the maintenance wash is complete, repeat the volume test.
Document # 15027617 v04Material # 20024228
For Research Use Only. Not for use in diagnostic procedures.
Perform a Maintenance Wash on page
34.
45
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