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MiSeq System Guide
a
Remove the waste bottle and discard the contents appropriately. Return the waste bottle to the
reagent compartment.
b Slowly lower the sipper handle, making sure that the sippers lower into the wash bottle and waste
bottle.
c
Close the reagent compartment door.
4
Select Next. The first wash begins.
Perform Second Wash
Always use fresh wash solution for each wash step. Reusing wash solution from the previous wash can return
waste to the fluidics lines.
1
Prepare fresh wash solution with Tween 20 and laboratory-grade water, as follows:
a
Add 5 ml 100% Tween 20 to 45 ml laboratory-grade water. These volumes result in 10% Tween 20.
b Add 25 ml 10% Tween 20 to 475 ml laboratory-grade water. These volumes result in a 0.5% Tween
20 wash solution.
c
Invert five times to mix.
2
When the first wash is complete, remove the wash tray and wash bottle, and discard the remaining wash
solution.
3
Refill the wash components with fresh wash solution, as follows:
a
Add 6 ml wash solution to each reservoir of the wash tray.
b Add 350 ml wash solution to the 500 ml wash bottle.
4
Load the wash tray and wash bottle, as follows:
a
Slide the wash tray into the reagent chiller until it stops. Close the reagent chiller door.
b Load the wash bottle and slowly lower the sipper handle, making sure that the sippers lower into the
wash bottle and waste bottle.
c
Close the reagent compartment door.
5
Select Next. The second wash begins.
Perform Final Wash
1
Prepare fresh wash solution with Tween 20 and laboratory-grade water:
a
Add 5 ml 100% Tween 20 to 45 ml laboratory-grade water. These volumes result in 10% Tween 20.
b Add 25 ml 10% Tween 20 to 475 ml laboratory-grade water. These volumes result in a 0.5% Tween
20 wash solution.
c
Invert five times to mix.
2
When the second wash is complete, remove the wash tray and wash bottle, and discard the remaining
wash solution.
3
Refill the wash components with fresh wash solution:
a
Add 6 ml wash solution to each reservoir of the wash tray.
b Add 350 ml wash solution to the 500 ml wash bottle.
4
Load the wash tray and wash bottle:
Document # 15027617 v04 Material # 20024228
For Research Use Only. Not for use in diagnostic procedures.
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