User Manuals: illumina MiSeq System DNA Sequencing

User Manuals: illumina MiSeq System DNA Sequencing

Manuals and User Guides for illumina MiSeq System DNA Sequencing. We have 3 illumina MiSeq System DNA Sequencing manuals available for free PDF download: User Manual, System Manual

illumina MiSeq System User Manual

illumina MiSeq System User Manual (116 pages)

Combines proven sequencing by synthesis (SBS) technology with a revolutionary workflow that enables you to go from DNA to analyzed data in as little as eight hours

Brand: illumina | Category: Laboratory Equipment | Size: 2 MB

Table of Contents

Table of Contents

7
- Revision History
  3
  - Table of Contents
    7
- Chapter 1 Getting Started
  9
- Introduction
  10
- Components
  13
- Miseq Reagent Kits
  18
- Kit Contents and Storage Requirements
  20
- Flow Cell Types
  22
- Version Compatibility and Requirements
  23
- User-Supplied Consumables
  24
- Anti-Virus Software
  25
- Starting the Miseq
  26
Miseq Software

27
- Chapter 2 Miseq Software
  27
- Introduction
  28
- Miseq Concepts
  29
- Welcome Screen
  30
- Perform Wash Screen
  34
- Manage Files Screen
  36
- Run Options Screen
  38
- Manage Instrument Screen
  42
- Run Setup Screens
  46
- Sequencing Screen
  53
- Run Folders
  56
- Run Duration
  60
- Required Disk Space
  61
- Pausing or Stopping a Run
  62
- Secondary Analysis Options
  64
- Live Help
  67
Performing a Run

69
- Chapter 3 Performing a Run
  69
- Introduction
  70
- Miseq Workflow
  71
- Preparing Reagents
  73
- Loading Sample Libraries Onto Cartridge
  75
- Setting up a Run Using MCS
  76
- Cleaning the Flow Cell
  77
- Loading the Flow Cell
  79
- Loading Reagents
  81
- Starting the Run
  84
- Monitoring the Run
  85
Maintenance Procedures

87
- Chapter 4 Maintenance Procedures
  87
- Introduction
  88
- Performing a Post-Run Wash
  89
- Performing a Maintenance Wash
  91
- Performing a Standby Wash
  94
- Shutting down the Instrument
  97
Troubleshooting

99
- Chapter 5 Troubleshooting
  99
- Introduction
  100
- Resolving Run Setup Errors
  101
- Resolving RFID Read Failure
  103
- Troubleshooting Flow Rate Error
  105
- Performing a Volume Test
  106
- Measuring Expected Wash Volumes
  109
- Index
  111
- Technical Assistance
  115

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illumina MiSeq System System Manual

illumina MiSeq System System Manual (67 pages)

Brand: illumina | Category: Laboratory Equipment | Size: 5 MB

Table of Contents

Table of Contents
9
Chapter 1 Overview

11
- Introduction
  11
- Additional Resources
  11
- Components
  12
- Miseq Concepts
  14
- System Software
  15
- Secondary Analysis Options
  16
- Sequencing Analysis Viewer
  18
- Required Disk Space
  18
- Miseq Reagent Kit Overview
  18
Chapter 2 Getting Started

21
- Start the Miseq
  21
- Customize System Settings
  21
- Configure Notifications of Basespace Updates
  22
- Set Email Preferences
  22
- Set Default Folder Locations
  22
- User-Supplied Consumables
  23
Chapter 3 Sequencing

25
- Introduction
  25
- Run Duration
  25
- Miseq Workflow
  27
- Thaw Reagent Cartridge
  28
- Inspect the Reagent Cartridge
  29
- Denature and Dilute Libraries
  29
- Load Sample Libraries
  29
- Set up a Run Using MCS
  30
- Clean the Flow Cell
  31
- Load the Flow Cell
  33
- Load Reagents
  34
- Start the Run
  36
- Monitor the Run
  37
- Perform a Post-Run Wash
  39
Chapter 4 Maintenance

43
- Maintenance Frequency
  43
- Maintenance Frequency for the Veriseq PGS Workflow
  43
- Perform a Maintenance Wash
  44
- Perform a Standby Wash
  46
- Manage Files
  48
- Software Updates
  49
- Shut down the Instrument
  49
Appendix A Troubleshooting

51
Appendix B Output Files and Folders

57
- Run Folders
  57
- Miseqoutput Folder Contents RTA Folders and Files
  57
- Index
  61
- Technical Assistance
  65
- Document # 15027617 V04 Material # 20024228
  67

illumina MiSeq System User Manual

illumina MiSeq System User Manual (66 pages)

Sequencing System

Brand: illumina | Category: Recording Equipment | Size: 1 MB

Table of Contents

Revision History
3
Table of Contents
5
List of Tables
7
Chapter 1 Getting Started

9
- Introduction
  10
- Components
  12
- Starting the Miseq
  16
- Miseq Workflow
  18
- Consumables
  19
- Table 1 User-Supplied Consumables
  19
Chapter 2 Miseq Control Software

21
- Introduction
  22
- Miseq Control Software Interface
  23
- Stopping or Pausing a Run
  30
- Sample Sheets
  31
- Analysis Workflow
  32
- Run Folders
  33
- Available Disk Space
  34
Chapter 3 Performing a Run

35
- Introduction
  36
- Preparing the Miseq Reagent Cartridge
  37
- Setting up a Run
  39
- Monitoring the Run
  44
Chapter 4 Post-Run Procedures

45
- Introduction
  46
- Performing a Post-Run Wash
  47
- Performing a Maintenance Wash
  48
- Table 2 Maintenance Wash Duration
  48
- Unloading Run Components
  50
- Shutting down the Instrument
  51
Chapter 5 On-Instrument Analysis

53
- Introduction Primary Analysis Results Secondary Analysis Using Miseq Reporter
  53
- Table 3 Contents of Analysis Folder
  55
Chapter 6 Troubleshooting

59

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