User Manuals: illumina NextSeq 500 Genetic Sequencer

User Manuals: illumina NextSeq 500 Genetic Sequencer

Manuals and User Guides for illumina NextSeq 500 Genetic Sequencer. We have 1 illumina NextSeq 500 Genetic Sequencer manual available for free PDF download: System Manual

illumina NextSeq 500 System Manual

illumina NextSeq 500 System Manual (59 pages)

Brand: illumina | Category: Laboratory Equipment | Size: 1 MB

Table of Contents

Table of Contents
7
Chapter 1 Overview

9
- Introduction
  9
- Additional Resources
  9
- Instrument Components
  10
- Sequencing Consumables Overview
  12
Chapter 2 Getting Started

16
Chapter 3 Sequencing

20
- Introduction
  20
- Sequencing Workflow
  21
- Prepare the Reagent Cartridge
  21
- Prepare the Flow Cell
  22
- Prepare Libraries for Sequencing
  23
- Set up a Sequencing Run
  23
- Monitor Run Progress
  29
- Automatic Post-Run Wash
  31
Chapter 4 Maintenance

32
- Introduction
  32
- Perform a Manual Wash
  32
- Software Updates
  34
- Shut down the Instrument
  35
Appendix A Troubleshooting

37
- Introduction
  37
- Troubleshooting Files
  37
- Resolve Automatic Check Errors
  38
- Spent Reagents Container Is Full
  39
- Rehybridization Workflow
  39
- Custom Recipes and Recipe Folders
  41
- System Check
  41
- RAID Error Message
  43
- Configure System Settings
  43
Appendix B Real-Time Analysis

46
- Real-Time Analysis Overview
  46
- Real-Time Analysis Workflow
  47
- Sequencing Output Files
  50
  - Output Folder Structure
    53
  - Index
    55
  - Technical Assistance
    58

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