illumina MiSeq System Manual page 13

MiSeq System Guide
A Flow cell compartment—Contains the flow cell stage that houses the flow cell throughout the run. Flow cell
stage motors move the stage out of the enclosed optical module for flow cell loading and returns the stage
when the run begins.
B Enclosed optics compartment—Contains optical components that enable imaging of the flow cell.
C Status bar—Indicates flow cell status as ready to sequence (green), processing (blue), or needs attention
(orange).
D Touch screen monitor—Displays the control software interface for system configuration and run setup.
E External USB ports—Facilitates the transfer of files and data to the instrument computer from the touch
screen monitor.
F Reagent compartment—Contains reagents at proper temperatures, wash solutions, and a bottle for used
reagents. A magnetic latch secures the reagent compartment door.
The MiSeq interface guides you through the run setup steps using the touch screen monitor. Loading run
components requires access to the reagent compartment and the flow cell compartment.
Flow Cell Compartment
The flow cell compartment contains the flow cell stage, thermal station, and fluidics connections to the flow
cell. The flow cell stage holds the flow cell and the flow cell clamp secures and positions the flow cell. When
the flow cell clamp closes, two pins near the clamp hinge auto-position the flow cell.
The thermal station, located beneath the flow cell stage, controls changes in flow cell temperature required
for cluster generation and sequencing.
Figure 1 Flow Cell Compartment
A Flow Cell Stage
B Flow Cell Compartment Door
C Flow Cell Clamp
D Flow Cell
E Flow Cell Clamp Release Button
Reagent Compartment
The reagent compartment contains the reagent chiller, and positions for the wash buffer (PR2) bottle and the
waste bottle. To maintain a consistent temperature, open and close the reagent chiller only when instructed.
NOTE
The required temperature range of the reagent chiller is 2°C to 11°C.
Document # 15027617 v04 Material # 20024228
For Research Use Only. Not for use in diagnostic procedures.
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