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MiSeq System Guide
Activity Indicators
An activity indicator icon displays the activity the instrument is currently performing.
Figure 3 Activity Indicators
From left to right, the activity indicators represent the following activities:
Moving the Y-stage
u
Moving the Z-stage
u
Activating electronics functionality
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Using the camera
u
Pumping through the fluidics system
u
Sensor Indicators
Sensor indicators, which appear at the base of each interface screen, represent the status of instrument
components.
Figure 4 Sensor Indicators
From left to right, the sensor indicators represent the following components:
Flow cell compartment door in the closed or open positions
u
Temperature of the reagent chiller in °C
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Temperature of the flow cell in °C
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Status of BaseSpace
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Secondary Analysis Options
MiSeq sequencing data can be analyzed on the instrument computer using Local Run Manager, on a
networked server using BaseSpace
information about alignment, variants, and contig assemblies for each genome requested and for each
sample of a multisample run. If performing the VeriSeq
analysis.
BaseSpace and BaseSpace Onsite Overview
BaseSpace is the Illumina cloud computing environment. BaseSpace Onsite provides a computing
environment on a dedicated server, complete with run setup tools and analysis options.
Log in to BaseSpace or BaseSpace Onsite when you set up the sequencing run. When using BaseSpace or
BaseSpace Onsite, you have the additional option to store run data locally. For more information, see
Customize System Settings on page
Document # 15027617 v04 Material # 20024228
For Research Use Only. Not for use in diagnostic procedures.
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connection (not connected shown)
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Onsite, or on the cloud using BaseSpace. These applications produce
11.
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PGS workflow, use BlueFuse
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Multi software for
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