illumina MiSeq System Manual page 7

MiSeq System Guide
Document #
Part # 15027617 Rev. D
Document # 15027617 v04 Material # 20024228
For Research Use Only. Not for use in diagnostic procedures.
Date Description of Change
July 2012 Updated software descriptions to MCS v2.0.
Added the following new information:
• Added a section titled What's New in MCS to describe new
software features, interface changes, and workflow
changes
• Added catalog number and description of the MiSeq
Reagent Kit v2, 500 Cycles
• Added Version Compatibility and Requirements section
• Added description of MiSeq Expansion Pack, which is
required for 14-tile dual-surface flow cell imaging
• Added description of dual-surface flow cell tile numbering
• Added the PCR Amplicon analysis workflow for Nextera XT
libraries
• Added the use of 10% Tween 20 in wash procedures and
expected wash volumes
• Added the reagent cartridge version to the RFID read
failure procedure
Updated the following information:
• Changed reagent acronyms for IMF, CMF, and AMX to v2
reagent names IMS, CMS, and AMS, respectively
• Changed the PhiX concentration from 8 pM to 12.5 pM
• Changed the maximum recommended NaOH
concentration to 1 mM in final solution
• Noted that a maintenance wash is required to remove the
instrument from standby mode and begin the setup steps
for a subsequent run
• Removed Sample Sheet Parameters section and sample
sheet setup step in the workflow; Illumina recommends
creating the sample sheet prior to sample preparation (See
the MiSeq Sample Sheet Quick Reference Guide , part #
15028392 and the Illumina Experiment Manager User
Guide , part # 15031335).
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