Appendix; Temperature Calibration Procedure - Beckman Coulter Avanti J-20 XP Instruction Manual

Temperature Calibration
The Avanti J-20XP centrifuge specification for temperature control is
±2°C of the set temperature. That means that your sample will stay
within 2°C of set temperature at all times, after rotor and system
equilibration, described below. (During transient conditions, such as
acceleration and deceleration, the rotor temperature may be outside
this range.) The following procedure is provided for those cases in
which temperature control within ±1°C is required.
In this procedure, you will measure the temperature of your sample
after equilibrating the rotor temperature for a minimum of 1 hour.
Based on the test results, you can then adjust the centrifuge tempera-
ture setting to bring your sample as close as possible to the required
temperature.
1. For runs at other than ambient temperature, precool or prewarm
the rotor and test samples to the required temperature. The length
of precooling/prewarming time depends on how different the rotor
starting temperature is from the required run temperature.
2. Install the rotor with adapters, if applicable, and tubes or bottles
filled with sample buffer or water (if above 2°C).
3. Set the speed to 2000 rpm, the time to HOLD, and the temperature
to the required run temperature. Start the run.
4. After 30 minutes, set the required run speed, and allow the system
to run for at least 1 hour.
NOTE
The system must run for at least 1 hour before
the temperature of the buffer or water is
measured. The length of time required for equil-
ibration depends on the rotor and centrifuge
starting temperatures and the ambient room
temperature.

APPENDIX

Procedure
A-1